摘要:
The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are superficially porous chromatographic particulate materials comprising sized less than 2 microns.
摘要:
A sol-gel sorbent or chromatography stationary phase is a particulate metal oxide gel containing polymeric segments uniformly distributed throughout the metal oxide gel. The metal oxide gel is an oxide from silicone or other metal oxide that can have one of the valence bonds attached to an organic group and the remainder occupied by oxygens that can be provided as an oxide or an alkoxide or aryl oxide of the polymeric segments. The particles are used for an SPE sorbent or as a packing for a reversed phase high-performance liquid chromatography (RP-HPLC), a normal phase high-performance liquid chromatography (NP-HPLC) column or a hydrophilic interaction liquid chromatography (HILIC) column.
摘要:
The subject technology is directed to a CO2-based chromatography system and method for rapid determination of the levels and/or the presence or absence of steroids or steroid derivatives in a sample.
摘要:
The object of the present invention is to provide a packing material and a separation method that manifest excellent hydrophilic interactions.The present invention provides a packing material for hydrophilic interaction chromatography consisting of a modified support treated with the surface modifier represented by the following formula (6) or (7). (In this formula, m denotes 2-6 and n denotes 1-4. X1, X2, and X3, independent of each other, denote a methoxy group, ethoxy group, or halogen. Up to two of X1, X2, and X3 can be any of the following groups: a methyl group, ethyl group, propyl group, isopropyl group, butyl group, or isobutyl group.)
摘要翻译:本发明的目的是提供一种表现出优异的亲水相互作用的包装材料和分离方法。 本发明提供一种用于亲水相互作用色谱的包装材料,其由用下式(6)或(7)表示的表面改性剂处理的改性载体组成。 (在该式中,m表示2-6,n表示1-4,X 1,X 2和X 3彼此独立地表示甲氧基,乙氧基或卤素,直至X 1, X 2和X 3可以是以下任何一个基团:甲基,乙基,丙基,异丙基,丁基或异丁基。)
摘要:
A method for dynamic filtration of a pharmaceutical product is provided. The method includes using an unconditioned resin configured to selectively retain one or more components from a mixture having the pharmaceutical product and where the unconditioned resin is configured to be activated by a medium of the mixture. The method further includes the use of at least one positioning material disposed adjacent to the unconditioned resin, where the positioning material is configured to provide mechanical support to the resin to at least partially retain the resin in position.
摘要:
This analysis method includes: subjecting a sample solution of a dye-containing sample in an organic solvent to normal-phase liquid chromatography to separate the sample solution and detect separated components. The normal-phase liquid chromatography involves (B1) using a separation column filled with a column packing material which is prepared by modifying a base material with a polar modifying group, and (B2) using as an eluent a polar organic solvent containing an acid.
摘要:
This application discloses, in part, 1) a stationary phase column and compression designs for preparative chromatography, 2) a method of improving performance of silica gel chromatography by controlling the hydration of silica gel and acidifying the mobile phase, and 3) a method of extending the life of a silica gel column packing by cleaning or regenerating the silica gel stationary phase.
摘要:
The invention provides a method for performing off-line multi-dimensional separation and analysis of a heterogeneous biomolecular sample. The method includes separating the heterogeneous biomolecular sample into a plurality of fractions using an electrophoresis capillary. The plurality of fractions are then deposited onto one or more structures, which keep each of the plurality of fractions separate from one another. The plurality of fractions are then subjected to non-eluting conditions by titrating the at least one of the plurality of fractions to an acidic pH and adding an ion-pairing reagent to the fractions. At least one of the fractions is then introduced into a capillary reversed-phase electrophoresis capillary. The fraction is then separated in the capillary into a plurality of sub-fractions based on hydrophobicity of the sub-fractions. The sub-fractions are then analyzed to determine their constituent molecules.
摘要:
A process for the chromatographic interseparation of rhodium in admixture with at least one base metal from a feed solution includes complexes of these metals. The process includes contacting the solution with at least one chromatographic medium, and eluting one or more fractions such that at least one fraction includes rhodium complex substantially free from base metal contamination. The at least one chromatography medium includes a support functionalised with substituted amine groups which further include at least one hydrophilic group. Other platinum group metals can also be separated, both from one another and from base metals.
摘要:
This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.