公开(公告)号：US20240309064A1

公开(公告)日：2024-09-19

申请号：US18637124

申请日：2024-04-16

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/02 ,  G01N30/80

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20240294606A1

公开(公告)日：2024-09-05

申请号：US18638362

申请日：2024-04-17

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/02 ,  G01N30/80

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US12054533B2

公开(公告)日：2024-08-06

申请号：US17858629

申请日：2022-07-06

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K1/36 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/71 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to methods for producing aflibercept from a host cell cultured in a chemically defined medium (CDM) including purification of aflibercept, wherein aflibercept following purification includes aflibercept variants that have at least one oxidized amino acid residue selected from the group consisting of tryptophan, histidine and a combination thereof.

公开(公告)号：US20240174737A1

公开(公告)日：2024-05-30

申请号：US18436259

申请日：2024-02-08

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Qian Zhang

IPC: C07K16/16 ,  A61K47/68 ,  G01N30/72 ,  G01N30/80 ,  G01N33/68 ,  H01J49/16

CPC classification number: C07K16/16 ,  A61K47/6803 ,  G01N30/7266 ,  G01N30/80 ,  G01N33/6848 ,  H01J49/165

Abstract: Methods and system for protein characterization using online chromatography and electrospray ionization mass spectrometry are provided.

公开(公告)号：US11958894B2

公开(公告)日：2024-04-16

申请号：US18213779

申请日：2023-06-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US11940428B2

公开(公告)日：2024-03-26

申请号：US17680383

申请日：2022-02-25

Applicant: SHIMADZU CORPORATION

Inventor： Soichiro Tamaoki ,  Tomoyuki Yamazaki ,  Shohei Sato

IPC: G01N30/86 ,  G01N30/16 ,  G01N30/80 ,  G01N30/02

CPC classification number: G01N30/8651 ,  G01N30/16 ,  G01N30/80 ,  G01N2030/027

Abstract: A preparative liquid chromatograph including an analysis channel (2), an injector (6), a separation column (8), a detector (10), a plurality of fraction collectors (14-1 to 14-n), a switching valve (12) that switches fraction collectors (14-1 to 14-n) to be connected to the outlet of the detector (10) among the plurality of fraction collectors (14-1 to 14-n), and a controller (16) for controlling operations of the plurality of fraction collectors (14-1 to 14-n) and the switching valve (12). In a case where the number of remaining unused collection containers of the fraction collector in use, which is connected to the outlet of the detector, falls below the number of buffers which is an integer of two or more, the controller (16) is configured to switch the switching valve (12) while the fraction collector in use is not performing a peak collection operation.

公开(公告)号：US20240053307A1

公开(公告)日：2024-02-15

申请号：US18383180

申请日：2023-10-24

Applicant: Merative US L.P.

Inventor： Eric W. Brown ,  Maria Eleftheriou ,  Anca Sailer ,  Ching-Huei Tsou

IPC: G01N30/80 ,  G01N30/32 ,  G01N30/60

CPC classification number: G01N30/80 ,  G01N30/32 ,  G01N30/6073 ,  G01N2030/328 ,  G01N2030/324 ,  G01N2030/326 ,  G01N2030/025

Abstract: A mechanism is provided in a data processing system comprising a processor and a memory, the memory comprising instructions that are executed by the processor to specifically configure the processor to implement a repetitive portion identification and weighting engine. A machine learning model is trained for weighting repetitive portions of patient electronic medical records (EMRs). A repetitive portion identification component applies a plurality of templates to clinical notes of a patient EMR to identify one or more candidate portions that match at least one of the plurality of templates. A content analysis component performs content analysis on the one or more candidate portions to determine whether each given candidate portion is relevant. A weighting component assigns a relative weight to each given candidate portion based on relevance. A cognitive summary graphical user interface (GUI) generation component generates cognitive summary reflecting at least a subset of the one or more candidate portions of the patient EMR. The mechanism outputs the cognitive summary in a GUI to a user.

公开(公告)号：US20240044847A1

公开(公告)日：2024-02-08

申请号：US18221621

申请日：2023-07-13

Applicant: SHIMADZU CORPORATION

Inventor： Soichiro TAMAOKI ,  Tomoyuki YAMAZAKI ,  Shohei SATO

IPC: G01N30/16 ,  G01N30/74 ,  G01N30/72 ,  G01N30/80

CPC classification number: G01N30/16 ,  G01N30/74 ,  G01N30/72 ,  G01N30/80 ,  G01N2030/326

Abstract: Provided is a preparative liquid chromatograph capable of coping with a case where actual second delay time changes after start of a fractionation sequence. When a specific component detected as a peak in both a first detector and a second detector is injected during execution of a fractionation sequence, a control device executes maintenance operation of time difference information stored in an information storage area. In the maintenance operation, the control device executes peak determination as to whether or not a difference between first retention time and second retention time falls within a predetermined allowable range. By the above, the control device checks whether or not the second delay time from when a component is detected by the second detector to when the component reaches a fraction collector changes from a previous state.

公开(公告)号：US20230341358A1

公开(公告)日：2023-10-26

申请号：US18009946

申请日：2021-03-29

Applicant: Thermo Fisher Scientific (Shanghai) Instruments Co., Ltd. ,  Thermo Fisher Scientific (Suzhou) Instruments Co., Ltd.

Inventor： Hongfang Wang ,  Gang Li ,  Kun Fan ,  Chao Gong

IPC: G01N30/80 ,  G01N30/32

CPC classification number: G01N30/80 ,  G01N30/32 ,  G01N2030/326 ,  G01N2030/328 ,  G01N2030/027

Abstract: A method for determining the flow rate of a liquid flow in a liquid chromatography system. The liquid chromatography system sequentially comprises a fraction valve, a first sensor and a second sensor along a flow path direction, wherein a pipeline between the first sensor and the second sensor has a known inter-sensor pipeline volume. The method comprises: step A: emptying a pipeline between a liquid discharge port of the fraction valve and the first sensor; step B: guiding a liquid flow from a liquid discharge port of the fraction valve to pipelines at positions where the first sensor and the second sensor are located; and step C: determining the flow rate of the liquid flow on the basis of a time difference between a first moment when the first sensor detects a liquid-gas interface where the liquid flow interfaces with a gas and a second moment when the second sensor detects the liquid-gas interface, and an inter-sensor pipeline volume. Further provided is a fraction collector. The accuracy of flow rate measurement and the efficiency of fraction collection can be greatly improved by means of the liquid-gas interface provided by the liquid flow passing through the emptied liquid discharge pipeline.

公开(公告)号：US20230331812A1

公开(公告)日：2023-10-19

申请号：US18213779

申请日：2023-06-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  C07K1/113 ,  C12N5/00 ,  C12P21/06 ,  C07K16/28 ,  C07K16/22 ,  G01N30/80 ,  C07K1/18 ,  A61K38/18 ,  C07K1/36 ,  C07K1/22 ,  C07K1/16 ,  C12N15/66 ,  C12P21/02

CPC classification number: C07K14/71 ,  C07K1/113 ,  C12N5/0031 ,  C12N5/0018 ,  C12P21/06 ,  C07K16/283 ,  C07K16/22 ,  G01N30/80 ,  C07K1/18 ,  A61K38/1866 ,  C07K1/36 ,  C07K1/22 ,  C07K1/16 ,  C12N15/66 ,  C12P21/02 ,  C07K2317/622 ,  C07K2319/30 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.