公开(公告)号：US12174199B2

公开(公告)日：2024-12-24

申请号：US18383750

申请日：2023-10-25

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang

IPC: G01N33/68 ,  G01N30/02 ,  G01N30/72 ,  H01J49/16

Abstract: The present disclosure pertains to method and system of characterizing a protein using an electrospray ionization source.

公开(公告)号：US20240272128A1

公开(公告)日：2024-08-15

申请号：US18643509

申请日：2024-04-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Yuetian Yan ,  Shunhai Wang

IPC: G01N30/72 ,  G01N33/563 ,  G01N33/68

CPC classification number: G01N30/7266 ,  G01N33/563 ,  G01N33/6857

Abstract: Methods and system for identifying and quantifying antibody fragments and identifying the site of fragmentation on an antibody are provided herein.

公开(公告)号：US20240036056A1

公开(公告)日：2024-02-01

申请号：US18374492

申请日：2023-09-28

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Zhengqi Zhang ,  Yuetian Yan ,  Shunhai Wang ,  Jethro Prinston ,  Jennifer Nguyen

IPC: G01N33/68 ,  G01N30/72 ,  G01N30/74

CPC classification number: G01N33/6848 ,  G01N30/7233 ,  G01N30/74 ,  G01N2030/027

Abstract: The present invention relates to the field of protein characterization, and in particular to methods for identifying critical quality attributes of therapeutic proteins by implementing a workflow including using a competitive binding assay with insufficient capture molecule followed by LC-MS.

公开(公告)号：US20230357357A1

公开(公告)日：2023-11-09

申请号：US18214282

申请日：2023-06-26

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn Lawrence ,  Amy Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  C12N5/00 ,  C07K16/28 ,  C07K1/16 ,  G01N30/80 ,  C07K1/18 ,  C07K16/22 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K1/36 ,  C12P21/06 ,  C07K1/113 ,  C12N15/66 ,  G01N30/02

CPC classification number: C07K14/71 ,  C12N5/0018 ,  C07K16/283 ,  C07K1/16 ,  G01N30/80 ,  C12N5/0031 ,  C07K1/18 ,  C07K16/22 ,  C07K1/22 ,  C12P21/02 ,  A61K38/1866 ,  C07K1/36 ,  C12P21/06 ,  C07K1/113 ,  C12N15/66 ,  C07K2319/33 ,  C07K2319/30 ,  C07K2317/622 ,  G01N2030/027 ,  C12N2800/10

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20230266286A1

公开(公告)日：2023-08-24

申请号：US18104669

申请日：2023-02-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Yuetian Yan

IPC: G01N30/72 ,  G01N30/38

CPC classification number: G01N30/7266 ,  G01N30/38 ,  G01N30/7273 ,  G01N2030/027

Abstract: Disclosed are native liquid chromatography-mass spectrometry systems and methods of use. A native liquid chromatography-mass spectrometry system can include a liquid chromatography system capable of separating a sample; and an electrospray ionization mass spectrometry (ESI-MS) system in fluid communication with the liquid chromatography system, wherein the ESI-MS system comprises a multi-nozzle electrospray ionization emitter and a system for modifying a desolvation gas and a mass spectrometer, wherein the mass spectrometer is configured to receive ions and characterize mass to charge ratio of ions.

公开(公告)号：US20230045769A1

公开(公告)日：2023-02-09

申请号：US17863303

申请日：2022-07-12

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Zhengqi Zhang ,  Yuetian Yan ,  Shunhai Wang

IPC: G01N33/68 ,  G01N30/72

Abstract: The present invention relates to the field of protein characterization, and in particular to methods for identifying critical quality attributes of therapeutic proteins expressed in host cells by implementing a workflow including using a competitive binding assay with insufficient antigen followed by SCX-MS.

公开(公告)号：US20230041349A1

公开(公告)日：2023-02-09

申请号：US17858629

申请日：2022-07-06

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C07K1/16 ,  C07K1/36 ,  C12N5/00 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80

Abstract: The present disclosure pertains to compositions comprising anti-VEGF and methods for producing such compositions in chemically defined media and controlling amounts of certain oxidized aflibercept variants.

公开(公告)号：US20230032607A1

公开(公告)日：2023-02-02

申请号：US17863349

申请日：2022-07-12

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Yu Feng ,  Shunhai Wang

IPC: G01N33/68

Abstract: The present invention generally pertains to methods of determining the amino acid sequence of a protein. In particular, the present invention pertains to the use of position-selective dimethylation and liquid chromatography-mass spectrometry to enhance the signal of N-terminal peptides and shift the signal of N-terminal peptides and corresponding b ions, thus facilitating a determination of the sequence of N-terminal peptides.

公开(公告)号：US11525833B2

公开(公告)日：2022-12-13

申请号：US17033820

申请日：2020-09-27

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Yuetian Yan ,  Shunhai Wang

IPC: G01N30/72 ,  G01N33/68 ,  H01J49/00

Abstract: The present invention provides rapid, sensitive high-throughput methods and systems for characterizing peptides or proteins using hydrophobic interaction chromatography-coupled native mass spectrometry to improve manufacturing process of biopharmaceutical products, such as identifying impurities during antibody purification, monitoring post-translational modification variants during production, or characterizing drug-to-antibody ratio of antibody-drug conjugates. The separation profiles of the peptides or proteins are generated and compared to identify or qualify the peptides or proteins.

公开(公告)号：US11525823B2

公开(公告)日：2022-12-13

申请号：US16899940

申请日：2020-06-12

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Yuetian Yan

IPC: A61K39/00 ,  C07K16/18 ,  C07K16/28 ,  C12P21/02 ,  A61K38/27 ,  G01N33/50 ,  G01N33/68 ,  C07K1/04 ,  C07K16/06 ,  C12N5/071 ,  C12N5/12

Abstract: Systems and methods to characterize dimerization interfaces at the subdomain level of a protein are provided. An exemplary method includes digesting a protein dimer sample into subdomains, labeling the digested protein sample, isolating labeled dimeric and monomeric subdomain fragments, and peptide mapping the labeled sample to determine where the dimer fragments are labeled and where the dimer fragments are not labeled. Regions that show decreased labeling extents in the dimer fraction than that in the monomer fraction are likely involved or in close proximity to the dimerization interface.