公开(公告)号：US12180272B2

公开(公告)日：2024-12-31

申请号：US17666107

申请日：2022-02-07

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Tong Zhang ,  Erica Pyles ,  Michael Rosconi ,  Nina Liu ,  Supriya Patel ,  Eric Smith ,  Andrew Murphy ,  Chia-Yang Lin ,  Samuel Davis

IPC: C07K16/00 ,  A61K39/395 ,  A61K47/68 ,  C07K16/24 ,  C07K16/28 ,  C07K16/30 ,  C12P21/08 ,  C07K1/00

Abstract: Antigen binding molecules (ABMs) comprising Fab domains in non-native configurations, ABM conjugates comprising the ABMs and cytotoxic or cytostatic agents, pharmaceutical compositions containing the ABMs and ABM conjugates, methods of using the ABMs, ABM conjugates and pharmaceutical compositions for treating cancer, nucleic acids encoding the ABMs, cells engineered to express the ABMs, and methods of producing ABMs.

公开(公告)号：US12054533B2

公开(公告)日：2024-08-06

申请号：US17858629

申请日：2022-07-06

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K1/36 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/71 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to methods for producing aflibercept from a host cell cultured in a chemically defined medium (CDM) including purification of aflibercept, wherein aflibercept following purification includes aflibercept variants that have at least one oxidized amino acid residue selected from the group consisting of tryptophan, histidine and a combination thereof.

公开(公告)号：US11649273B2

公开(公告)日：2023-05-16

申请号：US17575585

申请日：2022-01-13

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal

IPC: C07K1/36 ,  C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C07K1/16 ,  C12N5/00 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20220275054A1

公开(公告)日：2022-09-01

申请号：US17575585

申请日：2022-01-13

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal

IPC: C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C07K1/16 ,  C07K1/36 ,  C12N5/00 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US11407813B2

公开(公告)日：2022-08-09

申请号：US17492440

申请日：2021-10-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li ,  Matthew Franklin

IPC: C07K1/16 ,  C07K1/36 ,  C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C12N5/00 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80 ,  G01N30/02

Abstract: The present disclosure pertains compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20210206833A1

公开(公告)日：2021-07-08

申请号：US16996127

申请日：2020-08-18

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li ,  Matthew Franklin

IPC: C07K14/71 ,  C07K1/22 ,  C12P21/06 ,  C07K1/18

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US12215148B2

公开(公告)日：2025-02-04

申请号：US16987487

申请日：2020-08-07

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Tong Zhang ,  Erica Pyles ,  Michael Rosconi ,  Nina Liu ,  Supriya Patel ,  Eric Smith ,  Andrew Murphy ,  Chiayang Lin ,  Samuel Davis

IPC: C07K16/24 ,  A61K39/395 ,  A61K47/68 ,  C07K16/28 ,  C07K16/30

Abstract: Antigen binding molecules (ABMs) comprising Fab domains in non-native configurations, ABM conjugates comprising the ABMs and cytotoxic or cytostatic agents, pharmaceutical compositions containing the ABMs and ABM conjugates, methods of using the ABMs, ABM conjugates and pharmaceutical compositions for treating cancer, nucleic acids encoding the ABMs, cells engineered to express the ABMs, and methods of producing ABMs.

公开(公告)号：US20240385188A1

公开(公告)日：2024-11-21

申请号：US18640720

申请日：2024-04-19

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Byung Chul Kim ,  Kun Lu ,  Nisha Palackal ,  Erica Pyles

IPC: G01N33/569 ,  G01N21/76 ,  G01N33/543 ,  G01N33/58 ,  G01N33/68

Abstract: The present invention generally pertains to methods of testing for the presence of anti-drug antibodies (ADAs) against therapeutic viral vectors. In particular, the present invention pertains to the use of biotin-labeled AAV as a capture reagent and ruthenium-labeled AAV as a detection reagent for the detection and quantification of ADAs against AAVs.

公开(公告)号：US20230041349A1

公开(公告)日：2023-02-09

申请号：US17858629

申请日：2022-07-06

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C07K1/16 ,  C07K1/36 ,  C12N5/00 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80

Abstract: The present disclosure pertains to compositions comprising anti-VEGF and methods for producing such compositions in chemically defined media and controlling amounts of certain oxidized aflibercept variants.

公开(公告)号：US11505594B2

公开(公告)日：2022-11-22

申请号：US17711723

申请日：2022-04-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li ,  Matthew Franklin ,  Shawn Lawrence ,  Amy Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K1/16 ,  C07K1/36 ,  C07K1/18 ,  C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C12N5/00 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80 ,  G01N30/02

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.