公开(公告)号：US12077570B2

公开(公告)日：2024-09-03

申请号：US18118558

申请日：2023-03-07

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal

IPC: C07K1/36 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/71 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins.

公开(公告)号：US12061180B2

公开(公告)日：2024-08-13

申请号：US18063128

申请日：2022-12-08

Applicant: Alexander Bozic

Inventor： Alexander Bozic

IPC: G01N30/84 ,  B01D15/40 ,  B01D45/08 ,  G01N30/06 ,  G01N30/80

CPC classification number: G01N30/84 ,  B01D15/40 ,  B01D45/08 ,  G01N30/06 ,  G01N2030/065 ,  G01N30/80

Abstract: The present invention relates to a gas-liquid separator for a chromatography system, comprising:



(a) a separating region having an inlet nozzle, a baffle unit and a gas distribution unit;
(b) a dividing region having a liquid outlet; and
(c) a gas discharge region having a gas outlet;
wherein the separating region is connected to the dividing region by a separating opening and the distance of the inlet nozzle from the baffle unit is greater than the smallest longitudinal extension of the separating opening and the inlet nozzle is configured such that a gas-liquid stream directed through the inlet nozzle can act on the baffle unit.




The present invention further relates to a chromatography system comprising a separator according to the invention and to a chromatography method wherein the separator is used.

公开(公告)号：US12054532B2

公开(公告)日：2024-08-06

申请号：US17458519

申请日：2021-08-26

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US11932684B2

公开(公告)日：2024-03-19

申请号：US18117634

申请日：2023-03-06

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Qian Zhang

IPC: C07K16/16 ,  A61K47/68 ,  G01N30/72 ,  G01N30/80 ,  G01N33/68 ,  H01J49/16

CPC classification number: C07K16/16 ,  A61K47/6803 ,  G01N30/7266 ,  G01N30/80 ,  G01N33/6848 ,  H01J49/165

Abstract: Methods and system for protein characterization using online chromatography and electrospray ionization mass spectrometry are provided.

公开(公告)号：US11732025B2

公开(公告)日：2023-08-22

申请号：US17994223

申请日：2022-11-25

Applicant: Regeneran Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn Lawrence ,  Amy Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C12N5/00 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20230235019A1

公开(公告)日：2023-07-27

申请号：US18116166

申请日：2023-03-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaime Grapel

IPC: C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C07K1/16 ,  C07K1/36 ,  C12N5/00 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/18 ,  C07K16/28 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80

CPC classification number: C07K14/71 ,  C12P21/06 ,  C07K1/113 ,  C07K1/16 ,  C07K1/36 ,  C12N5/0018 ,  C12N5/0031 ,  C07K1/18 ,  C07K1/22 ,  C12P21/02 ,  A61K38/1866 ,  C07K16/283 ,  C12N15/66 ,  C07K16/22 ,  G01N30/80 ,  C07K2319/30 ,  C12N2800/10 ,  C07K2317/622 ,  C07K2319/33 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins.

公开(公告)号：US20190184327A1

公开(公告)日：2019-06-20

申请号：US16325855

申请日：2016-08-15

Applicant: Agilent Technologies, Inc.

Inventor： Edwin E. Wikfors ,  Samuel O. Colgate

IPC: B01D53/04 ,  G01N30/80 ,  B01D15/08

CPC classification number: B01D53/0423 ,  B01D15/08 ,  B01D15/24 ,  B01D15/40 ,  B01D19/00 ,  B01D19/0042 ,  B01D19/0063 ,  B01D53/025 ,  G01N30/80 ,  G01N30/84

Abstract: A gas-liquid separator includes a fluid inlet, a shell including an inside surface enclosing an interior space, an outlet structure with fingers converging toward a longitudinal axis, and a dripper including a dripper tip. The fingers terminate at fingertips located proximate to an outside surface of the dripper. Gas exit ports are defined between adjacent fingers, and by the dripper. The gas-liquid separator defines a liquid flow path from the fluid inlet, along the inside surface, along one or more of the fingers, converging along the dripper outside surface, and to the dripper tip. The gas-liquid separator also defines a gas flow path from the fluid inlet, through the interior space, and through the gas exit ports. The gas-liquid separator may be utilized in fluid separation systems such as liquid chromatography or supercritical fluid chromatography/extraction systems.

公开(公告)号：US20180238841A1

公开(公告)日：2018-08-23

申请号：US15750856

申请日：2016-05-23

Applicant: Shimadzu Corporation

Inventor： Tomoyuki YAMAZAKI

IPC: G01N30/38 ,  G01N30/80 ,  G01N30/32 ,  B01D15/24 ,  B01D15/14 ,  B01D15/16

CPC classification number: G01N30/38 ,  B01D15/14 ,  B01D15/161 ,  B01D15/163 ,  B01D15/247 ,  G01N30/32 ,  G01N30/80 ,  G01N30/84 ,  G01N2030/085 ,  G01N2030/328 ,  G01N2030/385

Abstract: A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.

公开(公告)号：US20180207549A1

公开(公告)日：2018-07-26

申请号：US15873280

申请日：2018-01-17

Applicant: Waters Technologies Corporation

Inventor： Xiangjin Song ,  James P. Murphy ,  Keith Fadgen ,  Wade P. Leveille ,  Joseph D. Michienzi ,  Jonathan L. Belanger ,  Moon Chul Jung ,  Abhijit Tarafder

IPC: B01D15/14 ,  B65D1/02 ,  B65D23/14

CPC classification number: B01D15/14 ,  B01L3/545 ,  B01L9/00 ,  B01L2300/021 ,  B65D1/0223 ,  B65D23/14 ,  B65D2501/0081 ,  G01N30/26 ,  G01N30/80 ,  G01N35/00732

Abstract: Solvent containers and solvent container trays for chromatography systems are described for providing control over solvent supply and waste collection. Designated solvent containers and exclusively designated solvent containers provide solvents for use by chromatography systems. Control over solvent supply is achieved by requiring matched container shape and container receiving position shape within the tray, and additionally or alternatively, through solvent container coding readable by the solvent tray and chromatography system which provide information about the solvent container to the chromatography system.

公开(公告)号：US20170260518A1

公开(公告)日：2017-09-14

申请号：US15127903

申请日：2015-12-03

Applicant: HUBEI UNIVERSITY OF TECHNOLOGY

Inventor： Yajie TANG ,  Kaizhi JIA ,  Yanghua XU ,  Quan ZHANG ,  Hongmei LI

IPC: C12N9/88 ,  G01N30/50 ,  G01N30/80 ,  C12N15/81

CPC classification number: C12N9/88 ,  C12N15/81 ,  C12Y404/01011 ,  G01N27/447 ,  G01N30/50 ,  G01N30/80

Abstract: Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea. Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No. 1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 μM MTL·h−1·mg protein−1.