公开(公告)号：US20250067750A1

公开(公告)日：2025-02-27

申请号：US18787598

申请日：2024-07-29

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Abu Nahiyaan Navid ,  Yunlong Zhao ,  Yuan Mao ,  Ning Li

IPC: G01N33/68 ,  C12Q1/34 ,  G01N30/02 ,  G01N30/72 ,  G01N30/88 ,  G16B40/10

Abstract: The present invention generally pertains to methods of characterizing non-consensus glycosylation sites of a protein. In particular, the present invention pertains to the use of high-throughput automated processes through digestion, enrichment of glycopeptides by liquid chromatography-mass spectrometry for identifying identification of non-consensus glycosylation sites.

公开(公告)号：US20240309064A1

公开(公告)日：2024-09-19

申请号：US18637124

申请日：2024-04-16

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/02 ,  G01N30/80

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20240294606A1

公开(公告)日：2024-09-05

申请号：US18638362

申请日：2024-04-17

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/02 ,  G01N30/80

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US12054533B2

公开(公告)日：2024-08-06

申请号：US17858629

申请日：2022-07-06

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Andrew Tustian ,  Ankit Vartak ,  Thomas Daly ,  Erica Pyles ,  Nisha Palackal ,  Shunhai Wang ,  Ning Li

IPC: C07K1/36 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/71 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to methods for producing aflibercept from a host cell cultured in a chemically defined medium (CDM) including purification of aflibercept, wherein aflibercept following purification includes aflibercept variants that have at least one oxidized amino acid residue selected from the group consisting of tryptophan, histidine and a combination thereof.

公开(公告)号：US11958894B2

公开(公告)日：2024-04-16

申请号：US18213779

申请日：2023-06-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  A61K38/18 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/28 ,  C12N5/00 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  G01N30/02

CPC classification number: C07K14/71 ,  A61K38/1866 ,  C07K1/113 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/22 ,  C07K16/283 ,  C12N5/0018 ,  C12N5/0031 ,  C12N15/66 ,  C12P21/02 ,  C12P21/06 ,  G01N30/80 ,  C07K2317/622 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20240053359A1

公开(公告)日：2024-02-15

申请号：US18204801

申请日：2023-06-01

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Hongxia Wang ,  Haibo Qiu ,  Ning Li ,  Zhijie Wu

IPC: G01N33/68

CPC classification number: G01N33/6854 ,  G01N33/6851 ,  G01N2440/00 ,  G01N2333/98

Abstract: The present invention pertains to methods for characterizing proteins in a sample using native capillary electrophoresis-mass spectrometry. The present invention pertains to methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample, detecting and/or discriminating between antibodies in an antibody mixture, and characterizing monospecific antibody side products in a bispecific antibody sample.

公开(公告)号：US20230331812A1

公开(公告)日：2023-10-19

申请号：US18213779

申请日：2023-06-23

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Shunhai Wang ,  Ning Li ,  Hunter Chen ,  Amardeep Singh Bhupender Bhalla ,  Shawn M. Lawrence ,  Amy S. Johnson ,  Meghan Casey ,  Jaimie Grapel

IPC: C07K14/71 ,  C07K1/113 ,  C12N5/00 ,  C12P21/06 ,  C07K16/28 ,  C07K16/22 ,  G01N30/80 ,  C07K1/18 ,  A61K38/18 ,  C07K1/36 ,  C07K1/22 ,  C07K1/16 ,  C12N15/66 ,  C12P21/02

CPC classification number: C07K14/71 ,  C07K1/113 ,  C12N5/0031 ,  C12N5/0018 ,  C12P21/06 ,  C07K16/283 ,  C07K16/22 ,  G01N30/80 ,  C07K1/18 ,  A61K38/1866 ,  C07K1/36 ,  C07K1/22 ,  C07K1/16 ,  C12N15/66 ,  C12P21/02 ,  C07K2317/622 ,  C07K2319/30 ,  C12N2800/10 ,  G01N2030/027

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

公开(公告)号：US20230166199A1

公开(公告)日：2023-06-01

申请号：US17948496

申请日：2022-09-20

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Xiaojing Zheng ,  Reid O'Brien Johnson ,  Tyler Greer ,  Mengqi Hu ,  Rosalynn Molden ,  Yunli Hu ,  Yu Huang ,  Haibo Qiu ,  Ning Li

IPC: B01D15/18 ,  B01D15/24 ,  B01D15/38 ,  G01N30/72 ,  G01N30/88 ,  G01N33/68

CPC classification number: B01D15/1871 ,  B01D15/24 ,  B01D15/3809 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848

Abstract: Methods for characterizing host-cell proteins in a sample matrix are provided.

公开(公告)号：US20230083288A1

公开(公告)日：2023-03-16

申请号：US17896496

申请日：2022-08-26

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Gaoyuan Liu ,  Xiaojing Zheng ,  Ning Li

IPC: G01N33/573 ,  G01N33/543 ,  G01N33/68

Abstract: The present invention generally pertains to methods of detecting host cell proteins. In particular, the present invention pertains to the use of a novel activity-based protein profiling probe to identify host cell proteins with lipase activity in a pharmaceutical formulation.

公开(公告)号：US11592449B2

公开(公告)日：2023-02-28

申请号：US16534095

申请日：2019-08-07

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Sook Yen E ,  Haibo Qiu ,  Ning Li

IPC: G01N33/68 ,  G16H50/30 ,  G16B40/10

Abstract: The present disclosure provides methods and compositions for the determining the abundance and/or concentration of protein biomarkers in a biological sample.