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公开(公告)号:US12227604B2
公开(公告)日:2025-02-18
申请号:US17291304
申请日:2019-11-06
Applicant: JSR CORPORATION
Inventor: Takanori Kishida , Takaya Nishida
IPC: C08F228/04 , B01D15/20 , B01D15/38 , B01J20/26 , B01J20/281 , B01J20/285 , B01J20/30 , C07C323/12 , C07D327/00 , C07K1/22 , C07K14/765 , C07K16/32 , C08F8/34 , C08F220/32 , C12N9/36 , G01N33/545
Abstract: A carrier which may have excellent pressure resistance, and even when a protein ligand is not immobilized thereon, a high dynamic binding capacity to a target substance, and a high performance of separating a target substance from a biological sample. Such a carrier may include a polymer having a crosslinked structure containing a divalent group of formula (1): wherein R1 to R4 are independently a single bond or divalent hydrocarbon group, R5 and R6 are independently H or a hydrocarbon group, X is a thio group, sulfinyl group, sulfonyl group, oxy group, >N(—R31), >Si(—R32)2, >P(—R33), >P(═O)(—R34), >B(—R35), or >C(—R36)2 (R31 to R36 independently being H or hydrocarbon group), and * is a bond, provided that when both R1 and R3 or both R2 and R4 are a divalent hydrocarbon group, respectively, R1 and R3 or R2 and R4 may form a ring together with an adjacent carbon atom.
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公开(公告)号:US12214298B2
公开(公告)日:2025-02-04
申请号:US17048779
申请日:2019-04-17
Applicant: University of Houston System
Inventor: Richard C. Willson , Ujwal Patil , Binh V. Vu , Ekaterini Kourentzi , Mary Crum
Abstract: The present disclosure relates to the detection of analytes in high volumetric flow applications. Particular embodiments relate to the use of fluorescence polarization/anisotropy based for detection of analytes in a flow cell. In one testing format, an analyte of interest is probed with reagents containing fluorescent labeled recognition elements. When present in a sample or portion of a sample, the labeled analyte produces a shift in fluorescence polarization/anisotropy/intensity/lifetime as the output signal following the binding of the recognition elements to the analytes.
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公开(公告)号:US20250035532A1
公开(公告)日:2025-01-30
申请号:US18786335
申请日:2024-07-26
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor: Deepanshu Choudhary , Michael Rosconi , Erica Pyles
Abstract: The present invention provides methods to identify and quantify empty, partial and full capsids. The capsids can be purified from a crude AAV sample using affinity chromatography and eluted from the solid support. The purified capsids can then be subjected to density gradient equilibrium analytical ultracentrifugation (DGE-AUC) for characterization and quantitation of capsid content.
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公开(公告)号:US20250034204A1
公开(公告)日:2025-01-30
申请号:US18917874
申请日:2024-10-16
Applicant: Genentech, Inc.
Inventor: Hui F. LIU , Brian David KELLEY , Deanna E. MYERS , Beth MCCOOEY , Krista Marie PETTY
Abstract: The present invention provides methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant and formulations comprising the polypeptide purified by the methods. The methods for purifying include cation exchange material and/or mixed mode material.
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公开(公告)号:US12194432B2
公开(公告)日:2025-01-14
申请号:US18307991
申请日:2023-04-27
Applicant: BIO-RAD LABORATORIES, INC.
Inventor: Christopher Belisle , Hong Chen , Yueping Xu , Jiali Liao , Xuemei He
Abstract: Chromatography resins having mixed mode ligands and methods of using such resins are provided.
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公开(公告)号:US12187763B2
公开(公告)日:2025-01-07
申请号:US17173767
申请日:2021-02-11
Applicant: ImmunityBio, Inc.
Inventor: Hing C. Wong , Jin-an Jiao , Caitlin Prendes
IPC: C07K1/22 , B01D15/38 , B01D61/02 , B01D61/14 , B01D61/58 , C07K14/54 , C07K14/715 , C07K14/745 , C07K16/36
Abstract: An affinity chromatography resin comprising an anti-tissue factor antibody or antigen-binding fragment thereof attached to a base resin, and methods of using the same.
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公开(公告)号:US12163160B2
公开(公告)日:2024-12-10
申请号:US18333718
申请日:2023-06-13
Inventor: Martin Lock , Mauricio Alvira
IPC: C12N7/02 , B01D15/08 , B01D15/16 , B01D15/36 , B01D15/38 , B01J20/281 , B01J41/05 , B01J41/13 , B01J41/20 , B01J47/022 , C12N7/00 , C12N15/86 , C12N15/864 , G01N21/33
Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
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公开(公告)号:US12161993B2
公开(公告)日:2024-12-10
申请号:US16638512
申请日:2018-08-23
Applicant: JSR CORPORATION , JSR LIFE SCIENCES CORPORATION , JSR Life Sciences, LLC , JSR MICRO N.V.
Inventor: Tomoya Norinobu
IPC: B01J20/26 , B01D15/10 , B01D15/38 , B01J20/28 , B01J20/285 , B01J20/30 , B01J20/32 , C07K1/22 , C07K16/32
Abstract: To provide a chromatography carrier that has excellent antifouling properties and exhibits excellent pressure-resistant performance and shatter-resistant performance.
A chromatography carrier comprising: a polymer having a partial structure containing at least two groups represented by —C(═O)—NH—.-
公开(公告)号:US20240400612A1
公开(公告)日:2024-12-05
申请号:US18696701
申请日:2022-09-28
Applicant: Kashiv BioSciences, LLC
Inventor: Om Narayan , Mayankkumar Thakkar , Anuj Kumar
IPC: C07K1/22 , A61K38/00 , B01D15/16 , B01D15/32 , B01D15/36 , B01D15/38 , C07K1/18 , C07K1/20 , C07K14/705
Abstract: The present invention relates to purification process of pharmacologically active cytotoxic T lymphocyte-associated 4-immunoglobulin (CTLA4-lg) fusion protein by using three column chromatography steps that provides purified form of the fusion protein, substantially free of misfolded species, charge variants and aggregates. A method for purifying a fusion protein from a protein mixture comprising a fusion protein and high molecular weight (HMW) impurity.
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公开(公告)号:US12157873B2
公开(公告)日:2024-12-03
申请号:US18374435
申请日:2023-09-28
Applicant: Genzyme Corporation
Inventor: Kevin Brower , Michael Coolbaugh , Xhorxhi Gjoka , Chad Varner , Jason Walther
Abstract: Systems and devices for producing biological products and methods of using the same are provided. An example universal skid includes a rigid support and a plurality of flow-channel hardware sets. Each flow-channel hardware set couples with a respective flow channel of a plurality of flow channels for unit operations. The flow-channel hardware sets allow the flow channels to operate in parallel or in series for continuous processing over the unit operations. An example modular assembly includes one or more single-use kits for one or more unit operations and a universal skid coupled with the one or more single-use kits. An example manufacturing system includes multiple modular assemblies coupled with each other allowing continuous processing within each modular assembly, in between the modular assemblies, fully continuous processing across multiple unit operations.
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