公开(公告)号：US12179128B2

公开(公告)日：2024-12-31

申请号：US17620137

申请日：2020-06-18

Applicant: UCB BIOPHARMA SRL

Inventor： Annick Gervais ,  Joel Dubisy

IPC: B01D15/32 ,  B01D15/16 ,  G01N30/34 ,  G01N30/88

Abstract: The current application provides a rapid and simple method for measuring residual pDADMAC in a sample containing a recombinant protein of interest based on the combination of use of reversed-phase hydrophobic interaction HPLC and charged aerosol detection.

公开(公告)号：US12130267B2

公开(公告)日：2024-10-29

申请号：US17296642

申请日：2019-10-30

Applicant: SHIMADZU CORPORATION

Inventor： Tetsuo Tanigawa ,  Natsuyo Asano ,  Zhao Qi Zhan ,  Yin Ling Chew ,  Jun Xiang Lee ,  Jie Xing

IPC: G01N30/34 ,  B01D15/32 ,  G01N30/02 ,  G01N30/60 ,  G01N30/72 ,  G01N30/88

CPC classification number: G01N30/34 ,  B01D15/325 ,  G01N30/7233 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/6013 ,  G01N2030/884

Abstract: A large number of kinds of azo compounds which are representative hazardous substances in fiber products are divided into two groups. The compounds included in the first group are detected by an MRM measurement by a tandem mass spectrometer unit (12) in a measurement section (10) while a two-liquid gradient elution under an acidic condition is performed in a liquid chromatograph unit (11), using an aqueous ammonium acetate solution as mobile phase A, and a mixture of acetonitrile and an aqueous ammonium acetate solution as mobile phase B. On the other hand, the compounds included in the second group are detected by an MRM measurement while a two-liquid gradient elution under a neutral or weakly basic condition is performed using an aqueous ammonium bicarbonate solution as mobile phase A and acetonitrile as mobile phase B. An exhaustive quantitative analysis for major azo compounds can be achieved by performing the two analyses for the same sample. An efficient test with a shortened analysis period can thereby be performed.

公开(公告)号：US20240319146A1

公开(公告)日：2024-09-26

申请号：US18574223

申请日：2022-07-01

Applicant: PEPTISTAR INC. ,  SEKISUI MEDICAL CO., LTD.

Inventor： Yoshitaka NEMOTO ,  Hitoshi ABE ,  Toshihiro MORI

IPC: G01N30/26 ,  B01D15/32 ,  B01D15/40 ,  B01D15/42 ,  C07K1/20 ,  G01N30/02 ,  G01N30/88

CPC classification number: G01N30/26 ,  B01D15/32 ,  B01D15/40 ,  B01D15/42 ,  C07K1/20 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/8831

Abstract: To provide a means capable of simultaneously and accurately analyzing a component derived from a carrier for liquid phase peptide synthesis with, for example, a target peptide.
A method for simultaneously analyzing a carrier for liquid phase peptide synthesis, a component derived from the carrier for liquid phase peptide synthesis, an amino acid to which the carrier for liquid phase peptide synthesis is bound, and a peptide compound to which the carrier for liquid phase peptide synthesis is bound, with a target peptide or final target peptide, the analysis method using high-performance liquid chromatography or supercritical fluid chromatography using an alcohol as an eluent.

公开(公告)号：US12083498B2

公开(公告)日：2024-09-10

申请号：US16926102

申请日：2020-07-10

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Jacob N. Fairchild ,  Jason F. Hill ,  Giorgis Isaac ,  Michael F. Morris ,  Kevin D. Wyndham

IPC: B01D15/26 ,  B01D15/30 ,  B01D15/32 ,  B01D15/40 ,  B01D53/02 ,  B01J20/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/283 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07F7/18

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  C07F7/1804 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/32 ,  B01J20/3234 ,  B01J2220/54 ,  B01J2220/80

Abstract: The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.

公开(公告)号：US12060407B2

公开(公告)日：2024-08-13

申请号：US17025893

申请日：2020-09-18

Applicant: Cytune Pharma

Inventor： David Bechard ,  Guy De Martynoff

IPC: C07K14/705 ,  B01D15/14 ,  B01D15/32 ,  B01D15/36 ,  B01J41/20 ,  C07K1/18 ,  C07K1/20 ,  C07K14/54 ,  C07K14/715 ,  A61K38/00

CPC classification number: C07K14/7155 ,  B01D15/14 ,  B01D15/327 ,  B01D15/363 ,  B01J41/20 ,  C07K1/18 ,  C07K1/20 ,  C07K14/5443 ,  A61K38/00 ,  C07K2319/00 ,  B01D15/363 ,  B01D15/327

Abstract: The present invention relates to a method for preparing a composition comprising monomeric conjugates from a sample, said conjugate comprising (a) a polypeptide comprising the amino acid sequence of interleukin 15 or derivatives thereof, and (b) a polypeptide comprising the amino acid sequence of the sushi domain of IL-15Rα or derivatives thereof; wherein said method comprises the use of anion-exchange chromatography followed by a hydrophobic interaction chromatography; and to a pharmaceutical composition which can be obtained by such a method.

公开(公告)号：US20240157335A1

公开(公告)日：2024-05-16

申请号：US18282491

申请日：2022-03-09

Applicant: W.R. Grace & Co.-Conn.

Inventor： Keling ZHANG ,  Reno NGUYEN ,  Simon THOMAS

IPC: B01J20/287 ,  B01D15/32 ,  B01J20/10 ,  B01J20/28 ,  B01J20/32 ,  C07C37/82 ,  C07D311/80

CPC classification number: B01J20/287 ,  B01D15/325 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28061 ,  B01J20/28083 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3259 ,  B01J20/3293 ,  C07C37/82 ,  C07D311/80 ,  B01J2220/52

Abstract: A stationary phase for high-performance liquid chromatography including monofunctional primary silane functionalized silica provides improved separation performance for the preparative or process scale purification of cannabidiol (CBD) and tetrahydro-cannabinol (THC). Silica bonded with monofunctional primary silane(s) provides more efficient separation of these molecules through higher resolution values, reduced peak broadening and lower separation impedance, thus enabling higher purity product or more efficient purification process.

公开(公告)号：US20240092822A1

公开(公告)日：2024-03-21

申请号：US18548711

申请日：2022-03-02

Applicant: Ionis Pharmaceuticals, Inc.

Inventor： Andrew A. Rodriguez ,  Christopher Michael Gabriel

IPC: C07H21/02 ,  B01D15/16 ,  B01D15/20 ,  B01D15/32 ,  B01D15/42

CPC classification number: C07H21/02 ,  B01D15/166 ,  B01D15/203 ,  B01D15/325 ,  B01D15/426

Abstract: The present disclosure provides a process of separating designated oligomeric compounds from sample solutions comprising at least one contaminant In certain embodiments, the designated oligomeric compounds comprise a modified oligonucleotide and a conjugate group comprising at least one carbohydrate cluster. In certain embodiments, the present disclosure provides HPLC conditions that increase the separation of a designated oligomeric compound from at least one contaminant compared to standard HPLC conditions.

公开(公告)号：US20230415073A1

公开(公告)日：2023-12-28

申请号：US18458482

申请日：2023-08-30

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Dingjiang LIU ,  Lin LUO ,  Long XU

IPC: B01D15/30 ,  G01N33/68 ,  B01D15/32 ,  A61K39/395 ,  C07K16/00 ,  C07K16/06 ,  C07K16/10 ,  G01N30/02

CPC classification number: B01D15/30 ,  G01N33/6854 ,  B01D15/327 ,  A61K39/39591 ,  C07K16/00 ,  C07K16/065 ,  C07K16/10 ,  C07K16/06 ,  G01N30/02 ,  G01N2030/027

Abstract: The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.

公开(公告)号：US11795241B2

公开(公告)日：2023-10-24

申请号：US17103328

申请日：2020-11-24

Applicant: CyDex Pharmaceuticals, Inc.

Inventor： Vincent D. Antle ,  Charles Alan Watson ,  David W. Miles

IPC: C08B37/00 ,  B01D15/32 ,  C08L5/16 ,  C08B37/16 ,  A61K47/69 ,  A61K47/40

CPC classification number: C08B37/0006 ,  A61K47/40 ,  A61K47/6951 ,  B01D15/325 ,  C08B37/0003 ,  C08B37/0012 ,  C08L5/16

Abstract: The present invention relates to compositions comprising fractionated alkylated cyclodextrin compositions having a single degree of substitution, and processes for preparing and using the same.

公开(公告)号：US20230296620A1

公开(公告)日：2023-09-21

申请号：US17965406

申请日：2022-10-13

Applicant: Genentech, Inc.

Inventor： Keyang Xu ,  Jintang He ,  Surinder Kaur

IPC: G01N33/68 ,  G01N30/72 ,  G01N30/20 ,  G01N30/32 ,  G01N30/88 ,  G01N30/46 ,  B01D15/32 ,  B01D15/18

CPC classification number: G01N33/6848 ,  G01N30/7233 ,  G01N30/20 ,  G01N30/32 ,  G01N30/88 ,  G01N30/463 ,  B01D15/325 ,  B01D15/1878 ,  G01N2333/976 ,  G01N2030/027

Abstract: The present application relates to systems and methods for assaying presence of large molecule analytes, such as proteins, e.g., antibodies, antigens, receptors, and the like, using a targeted two-dimensional liquid chromatography, tandem mass spectrometry (2D-LC-MS/MS) system, optionally combined with affinity capture. In some aspects, the system is partially or fully automated. In some aspects, the system may allow detection of protein biomarkers (e.g., antibodies or antigens) from clinical or nonclinical biological tissue or fluid samples in the pg/mL to ng/mL range.