公开(公告)号：US20230296620A1

公开(公告)日：2023-09-21

申请号：US17965406

申请日：2022-10-13

Applicant: Genentech, Inc.

Inventor： Keyang Xu ,  Jintang He ,  Surinder Kaur

IPC: G01N33/68 ,  G01N30/72 ,  G01N30/20 ,  G01N30/32 ,  G01N30/88 ,  G01N30/46 ,  B01D15/32 ,  B01D15/18

CPC classification number: G01N33/6848 ,  G01N30/7233 ,  G01N30/20 ,  G01N30/32 ,  G01N30/88 ,  G01N30/463 ,  B01D15/325 ,  B01D15/1878 ,  G01N2333/976 ,  G01N2030/027

Abstract: The present application relates to systems and methods for assaying presence of large molecule analytes, such as proteins, e.g., antibodies, antigens, receptors, and the like, using a targeted two-dimensional liquid chromatography, tandem mass spectrometry (2D-LC-MS/MS) system, optionally combined with affinity capture. In some aspects, the system is partially or fully automated. In some aspects, the system may allow detection of protein biomarkers (e.g., antibodies or antigens) from clinical or nonclinical biological tissue or fluid samples in the pg/mL to ng/mL range.