公开(公告)号：US12123879B2

公开(公告)日：2024-10-22

申请号：US17352132

申请日：2021-06-18

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Tyler Greer ,  Milos Cejkov ,  Reid O'Brien Johnson ,  Xiaojing Zheng ,  Ning Li

IPC: G01N33/68 ,  G01N30/72

CPC classification number: G01N33/6848 ,  G01N30/7233 ,  G01N33/6854 ,  G01N2440/00

Abstract: The present disclosure provides a method for accurately measuring post-translational modifications in proteins such as antibodies. In particular, the method pertains to the use of heavy isotopic standards to generate a calibration curve to allow for accurate quantitation of a modified peptide. The method may be used to accurately quantify C-terminal truncation in antibodies using mass spectrometry.

公开(公告)号：US20240345094A1

公开(公告)日：2024-10-17

申请号：US18592661

申请日：2024-03-01

Applicant: Saint Louis University

Inventor： James Edwards ,  Christopher K. Arnatt ,  Michael Armbruster

IPC: G01N33/58 ,  G01N30/02 ,  G01N30/72

CPC classification number: G01N33/58 ,  G01N30/7233 ,  G01N2030/027 ,  G01N2458/15

Abstract: The present disclosure provides constant-mass isobaric tags and methods for their use in multiplexed, double fragmentation mass spectrometry. The tags can be used for various aldehyde-, thiol-, amine-, and acid-containing analytes and can be used to quantify their relative amounts in different samples. Up to 192 samples can be combined for analysis.

公开(公告)号：US20240345043A1

公开(公告)日：2024-10-17

申请号：US18577407

申请日：2022-07-05

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhasish Purkayastha ,  Yves Le Blanc

IPC: G01N30/72 ,  B01D15/08 ,  G01N30/86 ,  H01J49/00

CPC classification number: G01N30/7233 ,  B01D15/08 ,  G01N30/8682 ,  H01J49/004

Abstract: One or more known compounds are separated from a mixture using a separation device that allows processor-controlled adjustment of a separation parameter. The separated compounds are ionized and, for each cycle of a plurality of cycles, a mass spectrometer executes on the ion beam a series of MRM transitions read from a list. Two or more contiguous groups of MRM transitions to be monitored separately are received. Each group includes at least one sentinel transition that identifies a next group that is to be monitored and identifies a value for the separation parameter for the next group. A first group is placed on the list. When a sentinel transition of the first group is detected, a next group identified by the sentinel transition is placed on the list and the separation parameter is adjusted to a value identified by the sentinel transition for the next group.

公开(公告)号：US20240329017A1

公开(公告)日：2024-10-03

申请号：US18612773

申请日：2024-03-21

Applicant: Thermo Fisher Scientific (Bremen) GmbH

Inventor： Daniel Felsmann ,  Thomas Wehkamp

IPC: G01N30/72 ,  G01N30/02

CPC classification number: G01N30/7233 ,  G01N2030/027

Abstract: An interface for coupling a liquid chromatography device to a mass spectrometry device comprises a reactor for chemically converting analytes eluting from the liquid chromatography device and a housing for accommodating the reactor. The reactor is arranged on a tray in the housing, which tray is slidable in a substantially horizontal plane relative to the housing so as to facilitate access to the reactor.

公开(公告)号：US20240272173A1

公开(公告)日：2024-08-15

申请号：US18568398

申请日：2022-06-08

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Thomas COVEY ,  Yuzhu GUO ,  Chang LIU

IPC: G01N33/68 ,  G01N30/06 ,  G01N30/72

CPC classification number: G01N33/6851 ,  G01N30/06 ,  G01N30/7233

Abstract: In one aspect, a method of introducing a sample into an open port interface (OPI) of a mass spectrometer is disclosed, which includes mixing the sample with a solvent in which a matrix of an aqueous phase of the sample is immiscible and in which at least a target analyte, when present in the sample, is miscible so as to extract at least a portion of the target analyte into said at least one solvent, thereby generating a multi-phase liquid having said aqueous phase and one or more organic phases, wherein at least one of those organic phases contains at least a portion of the target analyte. In some embodiments, the method further calls for ejecting a plurality of droplets from at least one of the phases of the multi-phase liquid for introduction into the OPI of the mass spectrometer.

公开(公告)号：US12051582B2

公开(公告)日：2024-07-30

申请号：US18468380

申请日：2023-09-15

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joseph D. Michienzi ,  Sylvain Gilles Cormier

IPC: H01J49/00 ,  G01N30/72 ,  H01J49/04 ,  H01J49/16

CPC classification number: H01J49/0495 ,  G01N30/7206 ,  G01N30/7233 ,  H01J49/0009 ,  H01J49/165

Abstract: Exemplary embodiments may deploy a valve that introduces a sample of a calibrant coaxially with flow exiting a source of a mobile phase flow, such as a liquid chromatography (LC) column, on a path to an ion source for the mass spectrometer (MS). The valve may be positioned remotely on a branch that has a junction with the path leading form the source of the mobile phase flow to the ion source. Alternatively, the valve may be positioned in line on the flow path from the source of the mobile phase flow to the ion source of the MS. A novel five port valve design may be employed. With this valve design, a first position of the valve allows a sample loop for the calibrant to be filled. In a second position, the calibrant is added coaxially to the flow from the source of the mobile phase to the MS. In a third position of the valve, diversion of or infusion to a post-source flow is enabled.

公开(公告)号：US12050221B2

公开(公告)日：2024-07-30

申请号：US18111336

申请日：2023-02-17

Applicant: UT-Battelle, LLC

Inventor： John F. Cahill

IPC: G01N33/68 ,  G01N30/72

CPC classification number: G01N33/6848 ,  G01N30/7233 ,  G01N2560/00

Abstract: A method for analyzing single cells by mass spectrometry includes the steps of providing a plurality of cells in a liquid medium and placing the cells and liquid medium in a single cell isolation and ejection system. Liquid medium containing a single cell is released from the single cell isolation and ejection system. The liquid medium and single cell are captured in a capture probe containing a flowing capture probe solvent. The cell is lysed by a lysis inducer in the capture probe to disperse single cell components into the medium. The lysed single cell components are transported to a mass spectrometer, where the lysed single cell components entering the mass spectrometer are spatially and temporally separated from any dispersed components of another single cell from the sample entering the mass spectrometer. Mass spectrometry is conducted on the lysed single-cell components. A system for analyzing single cells by mass spectrometry is also disclosed.

公开(公告)号：US12027358B2

公开(公告)日：2024-07-02

申请号：US17216988

申请日：2021-03-30

Applicant: Shimadzu Corporation

Inventor： Wenjian Sun ,  Ran Qiu ,  Xiaoqiang Zhang

IPC: H01J49/00 ,  G01N30/72

CPC classification number: H01J49/0072 ,  G01N30/7206 ,  G01N30/7233 ,  H01J49/0036

Abstract: The invention provides a mass spectrometry analysis method and a mass spectrometry system, including: a first sample separation step, separating a sample by a first sample separation device to obtain first isolates; a second sample separation step, introducing ionized first isolates into a second sample separation device for further separation based on a difference in ion mobility to obtain second isolates; and a mass spectrometry analysis step, performing mass spectrometry detection at least on daughter ions obtained from dissociation of the second isolates; in the second sample separation step, the second sample separation device operates in a filter mode, and screens out and releases ions having ion mobility within a specified range from received second isolates. The mass spectrometry analysis method can improve the dynamic range of mass spectrometry analysis.

公开(公告)号：US20240201147A1

公开(公告)日：2024-06-20

申请号：US17794629

申请日：2022-03-30

Applicant: REMEGEN CO., LTD.

Inventor： Xuejing YAO ,  Li WANG ,  Wenlong SUN ,  Guiping QI

IPC: G01N30/72 ,  B01D15/08 ,  G01N30/02

CPC classification number: G01N30/7233 ,  B01D15/08 ,  G01N2030/027

Abstract: The present disclosure relates to a method for detecting TCEP content in ADC by LC-MS/MS. The ADC samples are derivatized by Ellman reaction, and then the TCEP content is measured by LC-MS/MS. This method can exclude the interference caused by the substances that may react with Ellman in complex ADC samples, which provides with no need for sample pretreatment, and with strong specificity.

公开(公告)号：US12014803B2

公开(公告)日：2024-06-18

申请号：US15733972

申请日：2019-05-31

Applicant: Waters Technologies Ireland Limited

Inventor： Russell J. Mortishire-Smith ,  Johannes P. C. Vissers

IPC: G16C20/20 ,  G01N27/623 ,  G01N30/72 ,  G16C20/90 ,  G16C60/00 ,  G01N30/86

CPC classification number: G16C20/20 ,  G01N27/623 ,  G01N30/7233 ,  G16C20/90 ,  G16C60/00 ,  G01N30/8686

Abstract: Techniques and apparatus for generating consensus libraries for sample matrices (Flow A) and using the consensus libraries to determine unknown-unidentified components of a sample (Flow B) are described. For example, in an embodiment, an apparatus may include at least one memory, and processing circuitry (220) coupled to said at least one memory, wherein said processing circuitry is adapted to receive a plurality of sample matrix data sets (210a-210n) for a sample matrix generated via mass analysis of said sample matrix, and to generate a consensus library (220) for the sample matrix based on said plurality of sample matrix data sets, the consensus library comprising a plurality of known-unidentified components for the sample matrix.