公开(公告)号：US20240310382A1

公开(公告)日：2024-09-19

申请号：US18736894

申请日：2024-06-07

申请人： Wuxi Institute For Food Control ,  Jiangnan University

发明人： Yongwei Feng ,  Yi Zhang ,  Chenhui Lu ,  Haodong Wei ,  Wenlong Wang ,  Xiuping Yan ,  Jing Cui

IPC分类号： G01N33/68

CPC分类号： G01N33/6851 ,  G01N2333/958

摘要： The present disclosure discloses a standard characteristic polypeptide sequence for quantitatively detecting casein glycomacropeptide in a polypeptide product by mass spectrometry, and belongs to the technical field of inspection and detection. According to the present disclosure, three polypeptides for quantitatively detecting the casein glycomacropeptide are obtained through screening, which have amino acid sequences shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 respectively, and are suitable for quantitatively analyzing the content of the casein glycomacropeptide in a sample to be detected. According to the present disclosure, accurate quantitative analysis of the casein glycomacropeptide is realized, and the polypeptide sequence can be used for determining whether the content of the casein glycomacropeptide in the product reaches a standard or not.

公开(公告)号：US12085564B2

公开(公告)日：2024-09-10

申请号：US17051466

申请日：2019-05-09

申请人： BRISTOL-MYERS SQUIBB COMPANY

发明人： Naiyu Zheng ,  Ian MacQuarie Catlett ,  Jianing Zeng

IPC分类号： G01N33/68 ,  G01N30/02 ,  G01N33/543

CPC分类号： G01N33/54393 ,  G01N33/6851 ,  G01N2030/027

摘要： The present invention relates to assays for monitoring and measuring the binding of a drug of interest to a receptor. In this assay, a blood sample collected from a subject dosed with the compound of interest is incubated with a lysis solution containing a quencher. The drug bound and quencher bound receptor is then isolated from the lysed blood sample. The isolated drug bound and quencher bound receptor is digested to generate surrogate drug bound and quencher bound peptides. The amount of surrogate peptides is determined. Receptor occupancy can be determined by comparing the amount of drug bound surrogate peptide to the total of drug bound and quencher bound surrogate peptide.

公开(公告)号：US12078639B2

公开(公告)日：2024-09-03

申请号：US17863928

申请日：2022-07-13

申请人： AmberGen, Inc.

发明人： Mark J. Lim ,  Gargey Yagnik ,  Kenneth J. Rothschild

IPC分类号： G01N33/68 ,  C07C271/20 ,  C12Q1/6841

CPC分类号： G01N33/6851 ,  C07C271/20 ,  C12Q1/6841 ,  G01N2400/00 ,  G01N2458/15

摘要： The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

公开(公告)号：US20240242950A1

公开(公告)日：2024-07-18

申请号：US18285988

申请日：2022-04-06

申请人： VANDERBILT UNIVERSITY ,  THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE ARMY, WASHINGTON, DC ,  TRIAD NATIONAL SECURITY, LLC

发明人： Trevor C. Glaros ,  Phillip Mach ,  Richard Caprioli ,  Jeremy Norris

IPC分类号： H01J49/00 ,  G01N30/72 ,  G01N30/86 ,  G01N30/88 ,  G01N33/68

CPC分类号： H01J49/005 ,  G01N30/72 ,  G01N30/86 ,  G01N30/88 ,  G01N33/6851 ,  G01N2030/8813

摘要： Systems and devices are disclosed to generate a multidimensional mass fingerprint that allows for identification on a low-resolution mass spectrometer equipped with post-ionization fragmentation. For this approach, rather than interrogating a sample that is processed into peptides using a single high resolution MS scan as in traditional fingerprinting, a raw unprocessed sample (containing all biochemical species: lipids, proteins, peptides, and metabolites) is analyzed by combining Matrix-Assisted Laser Dissociation/Ionization (MALDI) ionization with low resolution tandem mass spectrometry. The proposed system combines improvements in MS hardware and software with state-of-the-art machine learning (ML) approaches to usher in rapid biological detection. This technique does not require any prior separation (liquid or gas chromatography) and is therefore rapid (e.g. less than 5 sec) and amenable to high throughput (e.g. greater than 384 samples/hr).

公开(公告)号：US20240219400A1

公开(公告)日：2024-07-04

申请号：US18557753

申请日：2022-04-29

申请人： Seer, Inc.

发明人： Asim Siddiqui ,  Daniel Hornburg ,  Craig Stolarczyk ,  Yina Li ,  Hongwei Xia

IPC分类号： G01N33/68 ,  G01N33/531 ,  G01N33/543

CPC分类号： G01N33/6851 ,  G01N33/531 ,  G01N33/54346 ,  G01N2333/976

摘要： The present disclosure provides a range of compositions and methods for enriching subsets of complex biological samples. Aspects of the present disclosure provide peptide-functionalized particles comprising affinities for subsets of biomolecules from complex biological samples. The present disclosure further provides methods for utilizing functionalized particles to fractionate and analyze complex biological samples.

公开(公告)号：US20240145027A1

公开(公告)日：2024-05-02

申请号：US18567573

申请日：2022-06-01

申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

发明人： Priyanka BANSAL ,  Ahmed BEN FALEH ,  Robert PELLEGRINELLI ,  Stephan WARNKE ,  Thomas RIZZO

IPC分类号： G16B15/00 ,  G01N21/35 ,  G01N27/623 ,  G01N33/68 ,  G16B40/10 ,  H01J49/04

CPC分类号： G16B15/00 ,  G01N21/35 ,  G01N27/623 ,  G01N33/6851 ,  G16B40/10 ,  H01J49/0481 ,  G01N2201/06113

摘要： A method, system, and computer-readable medium for identifying and creating a database of isomers and isobars of molecules including the steps of performing isomer or isobar separation on molecules to obtain separate isomeric or isobaric molecules, measuring mass-to-charge ratios (m/z) to obtain IR fingerprints of the separate isomeric or isobaric molecules, and storing first data on the mass-to-charge ratios (m/z) and/or the IR fingerprints of the separate isomeric or isobaric molecules to a database.

公开(公告)号：US11923082B2

公开(公告)日：2024-03-05

申请号：US17837442

申请日：2022-06-10

申请人： Hangzhou Calibra Diagnostics Co., Ltd. ,  Hangzhou Dian Medical Inspection Center Co., Ltd.

发明人： Huafen Liu ,  Ziqing Kong ,  Chao Zhang ,  Rongchang Chen ,  Yuning Zhu

IPC分类号： G16H40/63 ,  G01N33/68

CPC分类号： G16H40/63 ,  G01N33/6851 ,  G01N33/6893 ,  G01N2800/042

摘要： The present invention relates to a system for predicting gestational diabetes mellitus (GDM) of pregnant individuals, wherein the system comprises an operation module, and the operation model comprises a support vector regression model, and the system is used to predict the plasma glucose levels at 1 hour and/or 2 hours of oral glucose tolerance test (OGTT) by using a support vector regression developed prediction model generated by substituting the concentration of the biomarkers in fasting blood samples of pregnant individuals. The present invention provides biomarkers and biomarker-based diagnostic models for differential diagnosis of gestational diabetes mellitus (GDM), which can be applied to diagnosis or prediction of GDM in early stage and are of great significance to the prevention or treatment of GDM.

公开(公告)号：US20240012002A1

公开(公告)日：2024-01-11

申请号：US18335713

申请日：2023-06-15

申请人： Alexandra Ros ,  Jorvani Cruz Villarreal ,  Ana Egatz-Gomez ,  Todd Sandrin ,  Paul Coleman

发明人： Alexandra Ros ,  Jorvani Cruz Villarreal ,  Ana Egatz-Gomez ,  Todd Sandrin ,  Paul Coleman

IPC分类号： G01N33/68 ,  G01N1/28 ,  G01N33/53 ,  B01L3/00

CPC分类号： G01N33/6851 ,  G01N1/28 ,  G01N33/5304 ,  B01L3/502738 ,  G01N2001/284 ,  B01L2300/0816 ,  B01L2300/049 ,  B01L2400/0638 ,  G01N2333/46

摘要： Described herein are systems and methods for a microfluidic immunoassay for in situ mass spectrometry analysis of intracellular protein biomarkers in tissue. In some embodiments, the tissue may comprise human brain tissue. In some embodiments, the protein biomarkers may comprise Aβ species comprising monomers and oligomers of Aβ1-42, Aβ1-40, Aβ1-39, Aβ2-43, or combinations thereof. In some embodiments, the systems and methods may comprise laser capture microdissection (LCM) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

公开(公告)号：US20230393134A1

公开(公告)日：2023-12-07

申请号：US18235943

申请日：2023-08-21

申请人： SHIMADZU CORPORATION

发明人： Yuko FUKUYAMA

IPC分类号： G01N33/569 ,  G01N33/68 ,  H01J49/00

CPC分类号： G01N33/56916 ,  G01N33/6851 ,  H01J49/0036

摘要： A method for analyzing a microorganism including an identification step for determining which of Abony and Pakistan which are two serotypes of Salmonella bacteria is contained in a sample which contains either Abony or Pakistan, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Minnesota, Infantis and Brandenburg which are three serotypes of Salmonella bacteria is contained in a sample which contains Minnesota, Infantis or Brandenburg, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of Schwarzengrund and Montevideo which are two serotypes of Salmonella bacteria is contained in a sample which contains either Schwarzengrund or Montevideo, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample.

公开(公告)号：US20230375566A1

公开(公告)日：2023-11-23

申请号：US17914621

申请日：2021-03-29

申请人： UNIVERSITEIT GENT

发明人： Lynn VANHAECKE ,  Margot DE SPIEGELEER ,  Karen DE CLERCK ,  Jozefien GELTMEYER ,  Varoon SINGH

IPC分类号： G01N33/68 ,  A61B10/02 ,  G01N1/40 ,  B01D39/16

CPC分类号： G01N33/6851 ,  A61B10/02 ,  G01N1/405 ,  B01D39/1623 ,  B01D2239/025 ,  B01D2239/1233 ,  B01D2239/0421 ,  B01D2239/0428 ,  B01D2239/10 ,  B01D2239/0631 ,  B01D2239/0407

摘要： A fibrous mat is provided for use in taking a metabolome sample and for use in desorption of the metabolome sample. The fibrous mat includes an open network of at least one polymer-based nanofiber. The diameter of the at least one nanofiber ranges between 50 nm and 1500 nm, and a cover layer is provided at a top and/or at a bottom of the open network of at least one nanofiber to exclude particles with a size larger than 2000 Da from interacting with the open network of at least one nanofiber by blocking and/or excluding access to these particles.