公开(公告)号：US20230243765A1

公开(公告)日：2023-08-03

申请号：US18295761

申请日：2023-04-04

申请人： Alexandra Ros ,  Daihyun Kim ,  Diandra Doppler ,  Jorvani Cruz Villarreal ,  Richard Kirian ,  Reza Nazari ,  Sahir Gandhi

发明人： Alexandra Ros ,  Daihyun Kim ,  Diandra Doppler ,  Jorvani Cruz Villarreal ,  Richard Kirian ,  Reza Nazari ,  Sahir Gandhi

IPC分类号： G01N23/20025 ,  G01N23/201 ,  B01L3/02 ,  G01N35/10 ,  B01L3/00

CPC分类号： G01N23/20025 ,  G01N23/201 ,  B01L3/0241 ,  G01N35/1016 ,  B01L3/502715 ,  G01N2223/30 ,  G01N2223/604 ,  G01N2223/612 ,  G01N2223/637 ,  G01N2223/1016 ,  G01N2223/203 ,  G01N2223/054 ,  G01N2035/1034 ,  B01L2300/0645

摘要： A single-piece hybrid droplet generator and nozzle component for serial crystallography. The single-piece hybrid droplet generator component including an internally-formed droplet-generation channel, an internally-formed sample channel, a nozzle, and a pair of electrode chambers. The droplet-generation channel extends from a first fluid inlet opening to the nozzle. The sample channel extends from a second fluid inlet opening to the droplet-generation channel and joins the droplet-generation channel at a junction. The nozzle is configured to eject a stream of segmented aqueous droplets in a carrier fluid from the droplet-generation channel through a nozzle opening of the single-piece component. The pair of electrode chambers are positioned adjacent to the droplet-generation channel near the junction between the droplet-generation channel and the sample channel. The timing of sample droplets in the stream of fluid ejected through the nozzle is controlled by applying a triggering signal to electrodes positioned in the electrode chambers of the single-piece component.

公开(公告)号：US11649173B2

公开(公告)日：2023-05-16

申请号：US17237836

申请日：2021-04-22

申请人： Ana Egatz-Gomez ,  Alexandra Ros

发明人： Ana Egatz-Gomez ,  Alexandra Ros

IPC分类号： C01F17/36 ,  B01J19/12 ,  B01J19/00 ,  C09K11/02 ,  C01F17/265 ,  C09K11/77 ,  B82Y30/00 ,  B82Y40/00

CPC分类号： C01F17/36 ,  B01J19/0013 ,  B01J19/126 ,  C01F17/265 ,  C09K11/025 ,  C09K11/7773 ,  B01J2219/00033 ,  B01J2219/00141 ,  B01J2219/00162 ,  B01J2219/00166 ,  B82Y30/00 ,  B82Y40/00 ,  C01P2004/38 ,  C01P2004/64 ,  C01P2006/60

摘要： Synthesizing upconverting nanoparticles includes heating a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the upconverting nanoparticles. Core-shell upconverting nanoparticles are synthesized by combining the upconverting nanoparticles with a precursor solution comprising one or more rare earth salts, an alkali metal salt or alkaline earth salt, and a solvent comprising a plasticizer to yield a nanoparticle mixture, heating the nanoparticle mixture in a microwave reactor to yield a product mixture, and cooling the product mixture to yield the core-shell upconverting nanoparticles.

公开(公告)号：US20220112079A1

公开(公告)日：2022-04-14

申请号：US17497675

申请日：2021-10-08

申请人： Alexandra Ros ,  John Spence ,  Diandra Doppler ,  Garrett Nelson ,  Richard Kirian ,  Reza Nazari ,  Ana Egatz-Gomez ,  Mukul Sonker ,  Mohammad Rabbani

发明人： Alexandra Ros ,  John Spence ,  Diandra Doppler ,  Garrett Nelson ,  Richard Kirian ,  Reza Nazari ,  Ana Egatz-Gomez ,  Mukul Sonker ,  Mohammad Rabbani

IPC分类号： B81C3/00 ,  B81B1/00

摘要： A microfluidic device for use in a serial crystallography apparatus includes a nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.

公开(公告)号：US20190134631A1

公开(公告)日：2019-05-09

申请号：US16237158

申请日：2018-12-31

申请人： Alexandra Ros ,  Bahige G. Abdallah

发明人： Alexandra Ros ,  Bahige G. Abdallah

IPC分类号： B01L3/00 ,  G01N1/38

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/123 ,  B01L2300/16 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0655 ,  G01N1/38

摘要： A microfluidic apparatus, systems and methods for microfluidic crystallization based on gradient mixing. In one embodiment, the apparatus includes (a) a first layer, (b) a plurality of first channels and a plurality of vacuum chambers both arranged in the first layer, where the plurality of vacuum chambers are each coupled to at least one of the first channels, (c) a membrane having first and second surfaces, where the first surface of the membrane is coupled to the first layer, (d) a second layer coupled to the second surface of the membrane, (e) a plurality of wells and a plurality of second channels both arranged in the second layer, where the wells are each coupled to at least one of the plurality of second channels and (f) a plurality of barrier walls each disposed in the plurality of second channels and arranged opposite to one of the plurality of vacuum chambers.

公开(公告)号：US20240216914A1

公开(公告)日：2024-07-04

申请号：US18603030

申请日：2024-03-12

申请人： Alexandra Ros ,  Daihyun Kim ,  Jinghui Luo

发明人： Alexandra Ros ,  Daihyun Kim ,  Jinghui Luo

IPC分类号： B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N1/40 ,  G01N15/00

CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  B03C5/028 ,  B01L3/50273 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  G01N2001/4038 ,  G01N2015/0038

摘要： Sub-micrometer bioparticles are separated by size in a microfluidic channel utilizing a ratchet migration mechanism. A structure within the microfluidic channel includes an array of micro-posts arranged in laterally shifted rows. Reservoirs are disposed at each end of the microfluidic channel. A biased AC potential is applied across the channel via electrodes immersed into fluid in each of the reservoirs to induce a non-uniform electric field through the microfluidic channel. The applied potential comprises a first waveform with a first frequency that induces electro-kinetic flow of sub-micrometer bioparticles in the microfluidic channel, and an intermittent superimposed second waveform with a higher frequency. The second waveform selectively induces a dielectrophoretic trapping force to selectively impart ratchet migration based on particle size for separating the sub-micrometer bioparticles by size in the microfluidic channel.

公开(公告)号：US11944967B2

公开(公告)日：2024-04-02

申请号：US17524976

申请日：2021-11-12

申请人： Alexandra Ros ,  Daihyun Kim ,  Jinghui Luo

发明人： Alexandra Ros ,  Daihyun Kim ,  Jinghui Luo

IPC分类号： B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N1/40 ,  G01N15/00

CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  B03C5/028 ,  B01L3/50273 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  G01N2001/4038 ,  G01N2015/0038

摘要： Sub-micrometer bioparticles are separated by size in a microfluidic channel utilizing a ratchet migration mechanism. A structure within the microfluidic channel includes an array of micro-posts arranged in laterally shifted rows. Reservoirs are disposed at each end of the microfluidic channel. A biased AC potential is applied across the channel via electrodes immersed into fluid in each of the reservoirs to induce a non-uniform electric field through the microfluidic channel. The applied potential comprises a first waveform with a first frequency that induces electro-kinetic flow of sub-micrometer bioparticles in the microfluidic channel, and an intermittent superimposed second waveform with a higher frequency. The second waveform selectively induces a dielectrophoretic trapping force to selectively impart ratchet migration based on particle size for separating the sub-micrometer bioparticles by size in the microfluidic channel.

公开(公告)号：US10722889B2

公开(公告)日：2020-07-28

申请号：US16237158

申请日：2018-12-31

申请人： Alexandra Ros ,  Bahige G. Abdallah

发明人： Alexandra Ros ,  Bahige G. Abdallah

IPC分类号： C30B7/08 ,  B01L3/00 ,  G01N1/38

摘要： A microfluidic apparatus, systems and methods for microfluidic crystallization based on gradient mixing. In one embodiment, the apparatus includes (a) a first layer, (b) a plurality of first channels and a plurality of vacuum chambers both arranged in the first layer, where the plurality of vacuum chambers are each coupled to at least one of the first channels, (c) a membrane having first and second surfaces, where the first surface of the membrane is coupled to the first layer, (d) a second layer coupled to the second surface of the membrane, (e) a plurality of wells and a plurality of second channels both arranged in the second layer, where the wells are each coupled to at least one of the plurality of second channels and (f) a plurality of barrier walls each disposed in the plurality of second channels and arranged opposite to one of the plurality of vacuum chambers.

公开(公告)号：US20190184395A1

公开(公告)日：2019-06-20

申请号：US16226165

申请日：2018-12-19

申请人： Alexandra Ros ,  Daihyun Kim ,  Jinghui Luo

发明人： Alexandra Ros ,  Daihyun Kim ,  Jinghui Luo

IPC分类号： B01L3/00

CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/0406 ,  B01L2400/0424

摘要： Sub-micrometer bioparticles are separated by size in a microfluidic channel utilizing a ratchet migration mechanism. A structure within the microfluidic channel includes an array of micro-posts arranged in laterally shifted rows. Reservoirs are disposed at each end of the microfluidic channel. A biased AC potential is applied across the channel via electrodes immersed into fluid in each of the reservoirs to induce a non-uniform electric field through the microfluidic channel. The applied potential comprises a first waveform with a first frequency that induces electro-kinetic flow of sub-micrometer bioparticles in the microfluidic channel, and an intermittent superimposed second waveform with a higher frequency. The second waveform selectively induces a dielectrophoretic trapping force to selectively impart ratchet migration based on particle size for separating the sub-micrometer bioparticles by size in the microfluidic channel.

公开(公告)号：US20240012002A1

公开(公告)日：2024-01-11

申请号：US18335713

申请日：2023-06-15

申请人： Alexandra Ros ,  Jorvani Cruz Villarreal ,  Ana Egatz-Gomez ,  Todd Sandrin ,  Paul Coleman

发明人： Alexandra Ros ,  Jorvani Cruz Villarreal ,  Ana Egatz-Gomez ,  Todd Sandrin ,  Paul Coleman

IPC分类号： G01N33/68 ,  G01N1/28 ,  G01N33/53 ,  B01L3/00

CPC分类号： G01N33/6851 ,  G01N1/28 ,  G01N33/5304 ,  B01L3/502738 ,  G01N2001/284 ,  B01L2300/0816 ,  B01L2300/049 ,  B01L2400/0638 ,  G01N2333/46

摘要： Described herein are systems and methods for a microfluidic immunoassay for in situ mass spectrometry analysis of intracellular protein biomarkers in tissue. In some embodiments, the tissue may comprise human brain tissue. In some embodiments, the protein biomarkers may comprise Aβ species comprising monomers and oligomers of Aβ1-42, Aβ1-40, Aβ1-39, Aβ2-43, or combinations thereof. In some embodiments, the systems and methods may comprise laser capture microdissection (LCM) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

公开(公告)号：US11318487B2

公开(公告)日：2022-05-03

申请号：US15930239

申请日：2020-05-12

申请人： Alexandra Ros ,  Daihyun Kim ,  Austin Echelmeier ,  Jorvani Cruz Villarreal ,  Diandra Doppler ,  Richard Kirian ,  Reza Nazari

发明人： Alexandra Ros ,  Daihyun Kim ,  Austin Echelmeier ,  Jorvani Cruz Villarreal ,  Diandra Doppler ,  Richard Kirian ,  Reza Nazari

IPC分类号： B05B7/04 ,  G01N23/20008

摘要： Systems and methods for performing serial crystallography by providing an aqueous suspension of a crystal sample to a T-junction at a first flow rate and providing an immiscible oil fluid to the T-junction at a second flow rate. The aqueous suspension and the oil are combined at the T-junction to produce a co-flow output fluid including a parallel co-flow of the aqueous suspension and the oil in the same output channel. The co-flow output fluid is ejected as a jet stream through a nozzle and the sample flow rate in the crystal sample in the jet stream is adjusted by adjusting the first flow rate of the aqueous suspension and the second flow rate of the oil. By combining the aqueous sample and the oil in this manner, the output of the jet stream can be regulated for compatibility with X-ray free electron laser serial crystallography.