公开(公告)号：US12087566B2

公开(公告)日：2024-09-10

申请号：US18227417

申请日：2023-07-28

Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Livia Schiavinato Eberlin ,  Thomas Milner ,  Jialing Zhang ,  John Lin ,  John Rector ,  Nitesh Katta ,  Aydin Zahedivash

IPC: H01J49/04 ,  A61B10/00 ,  B01L3/00 ,  G01N30/72 ,  G01N33/487 ,  G01N33/574 ,  G01N33/68 ,  H01J49/00 ,  B01L3/02 ,  G01N1/02 ,  G01N1/40

CPC classification number: H01J49/0431 ,  A61B10/0045 ,  B01L3/502 ,  G01N30/72 ,  G01N33/487 ,  G01N33/574 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/04 ,  B01L3/0293 ,  B01L2200/0605 ,  B01L2400/0655 ,  G01N1/02 ,  G01N2001/028 ,  G01N2001/4061 ,  G01N2560/00

Abstract: Method and devices are provided for assessing tissue samples from a plurality of tissue sites in a subject using molecular analysis. In certain aspects, devices of the embodiments allow for the collection of liquid tissue samples and delivery of the samples for mass spectrometry analysis.

公开(公告)号：US12083521B2

公开(公告)日：2024-09-10

申请号：US16626647

申请日：2018-07-02

Applicant: BLINK AG

Inventor： Torsten Schulz ,  Eugen Ermantraut ,  Katrin Steinmetzer ,  Alrik Wolff

IPC: B01F35/513 ,  B01F23/411 ,  B01F31/20 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  C12M1/00 ,  C12M1/34 ,  G01N15/00 ,  G01N15/1433 ,  G01N15/14

CPC classification number: B01L7/52 ,  B01F23/4111 ,  B01F31/20 ,  B01F35/513 ,  B01L3/502776 ,  B01L3/505 ,  C12M41/14 ,  G01N15/1433 ,  B01L2200/0636 ,  B01L2200/0684 ,  B01L2300/042 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2300/1822 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0481 ,  B01L2400/049 ,  B01L2400/0655 ,  G01N2015/1493

Abstract: The present invention relates to a sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets and/or for performing biochemical reactions with or in such dispersion. The present invention furthermore relates to a device for incubating a dispersion of particles, cells or droplets and/or for performing a biochemical reaction therewith. Moreover, the present invention also relates to the use of a sample cartridge or of a device for generating and/or processing a dispersion of particles, cells or droplets. Moreover, the present invention relates to a method of processing a dispersion of particles, cells or droplets. Furthermore, the present invention relates to a method of generating a dispersion of droplets and to a method of generating a dispersion of solid or semi-solid particles.

公开(公告)号：US20240058813A1

公开(公告)日：2024-02-22

申请号：US17891636

申请日：2022-08-19

Applicant: Agilent Technologies, Inc.

Inventor： Jonathan Irribarren ,  Joel Myerson ,  Bo Curry

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655

Abstract: A fluid delivery device is configured to create a pressure differential across a compartment in which a liquid resides, causing the liquid to flow through the compartment. After a period of time, the fluid delivery device is configured to eliminate the pressure differential and thereby equilibrate the pressure across the compartment, with the use of a pressure equilibration channel that is separate from the compartment. The compartment may contain a packed bed of solid phase particles such as beads. In such case, the pressure differential causes the liquid to flow through the packed bed. The liquid may include chemical reagents or precursors that participate in chemical reactions on or at the solid phase particles. The reactions may relate to chemical synthesis, for example the synthesis of bio-chemicals such as nucleotides.

公开(公告)号：US11872559B2

公开(公告)日：2024-01-16

申请号：US16333297

申请日：2017-09-13

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

Inventor： Bart Deplancke ,  Johannes Bues ,  Riccardo Dainese ,  Marjan Biocanin

IPC: B01L3/00 ,  C12Q1/6806 ,  G06T7/00 ,  C12Q1/00

CPC classification number: B01L3/502753 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502784 ,  C12Q1/6806 ,  G06T7/0014 ,  B01L2300/0883 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2565/619 ,  C12Q2565/629

Abstract: The present invention concerns a microfluidic device or chip including at least one inlet for introducing at least one object into the device; an oil inlet for introducing an oil that supports droplet formation into the device or a droplet forming substance inlet for introducing a droplet forming substance into the device; a co-encapsulation area or structure where the at least one object is encapsulated by the droplet; a microfluidic tubing or channel for transporting the at least one object to an entrance of the co-encapsulation area or structure; an oil supporting droplet formation microchannel or droplet forming substance microchannel connected to the microfluidic tubing or channel to place a liquid of the microfluidic tubing or channel in direct contact with the oil that supports droplet formation or the droplet forming substance; and a droplet microchannel or tubing for transporting the droplet.

公开(公告)号：US11872554B2

公开(公告)日：2024-01-16

申请号：US16812961

申请日：2020-03-09

Applicant: ABBOTT DIAGNOSTICS SCARBOROUGH, INC.

Inventor： Alexander Schenk zu Schweinsberg ,  Austin Matthew Derfus ,  Justin Davidson ,  Karthikeyan Kumaravadivelu ,  Maulik Vinod Patel ,  Olaf Piepenburg ,  Catherine Jean Greenwood ,  Oliver Nentwich

IPC: G01N1/00 ,  B01L3/00 ,  G01N1/14 ,  G01N35/00 ,  G01N1/02 ,  G01N1/38 ,  G01N35/10

CPC classification number: B01L3/502715 ,  B01L3/5029 ,  G01N1/14 ,  G01N35/00069 ,  B01L2200/027 ,  B01L2200/082 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0478 ,  B01L2400/0655 ,  G01N1/38 ,  G01N2001/028 ,  G01N2035/00158 ,  G01N2035/1034

Abstract: A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.

公开(公告)号：US11813609B2

公开(公告)日：2023-11-14

申请号：US16845631

申请日：2020-04-10

Applicant: GENEWAVE

Inventor： Yann Marcy ,  Marc Artigue ,  Quentin Le Masne ,  Kevin Marq

IPC: B01L3/00 ,  B01L9/00 ,  C12Q1/6844

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L9/527 ,  C12Q1/6844 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/087 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/065 ,  B01L2400/0622 ,  B01L2400/0638 ,  B01L2400/0644 ,  B01L2400/0655

Abstract: A microfluidic cartridge for detecting one nucleic acid of a sample, including a plurality of functional volumes split into functional areas and a fluidic network of microchannels. At least three functional areas are fluidly connected to a central distribution hub of fluids by one or more hub-connected microchannels, the central distribution hub being capable of pumping and injecting fluids from a first functional area to a second functional area by passing through the central distribution hub; and at least three valves of hub-connected microchannels are arranged so that the at least three valves are adapted to be actuated mechanically by a single external cam-driven actuator.

公开(公告)号：US20230321655A1

公开(公告)日：2023-10-12

申请号：US18054484

申请日：2022-11-10

Applicant: Pacific Biosciences of California, Inc.

Inventor： Christopher Kilcoin ,  Kristin Dills ,  Rebecca McGinley

IPC: B01L3/00 ,  F04B23/02 ,  F04B1/00 ,  G01N30/60 ,  C12Q1/6869 ,  G01N30/88 ,  C12Q1/6806

CPC classification number: B01L3/502738 ,  B01L2400/0666 ,  F04B1/00 ,  G01N30/6091 ,  B01L3/50853 ,  C12Q1/6869 ,  B01L2200/16 ,  B01L2300/0861 ,  B01L3/50273 ,  B01L2400/0622 ,  B01L2300/0877 ,  B01L2400/0478 ,  G01N2030/8827 ,  C12Q1/6806 ,  B01L2300/0867 ,  B01L2400/0644 ,  B01L2400/0655 ,  B01L2400/0638 ,  F04B23/025

Abstract: A reagent cartridge including (a) a support having reservoirs; (b) a main channel within the support, the channel having first and second ends exiting the support; (c) a pump channel that connects to the main channel between the first and second ends; (d) a valve manifold in the support, including (i) a first passage at the first end of the main channel, (ii) a second passage at the second end of the main channel, (iii) a first master valve between the pump channel and the first end of the main channel, (iv) a second master valve between the pump channel and the second end of the main channel, and (v) reservoir valves for regulating flow from individual reservoirs to the main channel. The valves can be normally closed diaphragm valves formed by magnetic pistons attached to an elastomeric sheet that is sandwiched in the support.

公开(公告)号：US11732229B2

公开(公告)日：2023-08-22

申请号：US17100542

申请日：2020-11-20

Applicant: Massachusetts Institute of Technology

Inventor： Linda Griffith ,  David Trumper ,  Collin Edington ,  Gaurav Rohatgi ,  Duncan Freake ,  Luis Soenksen ,  Brij Mohan Bhushan

IPC: C12M1/32 ,  F04B23/04 ,  F04B43/12 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  F04B43/04 ,  F04B23/06 ,  F04B19/00

CPC classification number: C12M23/12 ,  B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  C12M25/14 ,  C12M29/10 ,  F04B19/006 ,  F04B23/04 ,  F04B23/06 ,  F04B43/043 ,  F04B43/12 ,  B01L2200/0621 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/086

Abstract: Fluidic multiwell bioreactors are provided as a microphysiological platform for in vitro investigation of multi-organ crosstalks for an extended period of time of at least weeks and months. The disclosed platform is featured with one or more improvements over existing bioreactors, including on-board pumping for pneumatically driven fluid flow, a redesigned spillway for self-leveling from source to sink, a non-contact built-in fluid level sensing device, precise control on fluid flow profile and partitioning, and facile reconfigurations such as daisy chaining and multilayer stacking. The platform supports the culture of multiple organs in a microphysiological, interacted systems, suitable for a wide range of biomedical applications including systemic toxicity studies and physiology-based pharmacokinetic and pharmacodynamic predictions. A process to fabricate the disclosed bioreactors is also provided.

公开(公告)号：US20230226547A1

公开(公告)日：2023-07-20

申请号：US17923280

申请日：2021-05-05

Applicant: Kilobaser GmbH

Inventor： Alexander MURER ,  Bernhard TITTELBACH ,  Martin JOST ,  Reinhard DIETHARDT

IPC: B01L3/00 ,  C07H1/00 ,  F16K99/00 ,  C07H21/04

CPC classification number: B01L3/502738 ,  C07H1/00 ,  F16K99/0015 ,  C07H21/04 ,  F16K99/0055 ,  B01L2300/0864 ,  B01L2400/0655 ,  B01L2300/0816

Abstract: A microfluidic valve includes a carrier layer and a flexible membrane layer arranged on a surface of the carrier layer. The surface of the carrier layer has a valve chamber in the form of a spherical cap and a membrane formed by the flexible membrane layer covers at least the valve chamber. A plurality of microfluidic channels opening into the valve chamber are formed in the surface of the carrier layer. Moreover, an inflow channel and an outflow channel are connected to one another by a microfluidic connection channel. The connection channel and the valve chamber are positioned relative to each other in such a way that in the closed state of the membrane, a fluid can flow from the inflow channel via the connection channel into the outflow channel to bridge the valve chamber, while the at least one supply channel is closed by the membrane.

公开(公告)号：US11691146B2

公开(公告)日：2023-07-04

申请号：US17898663

申请日：2022-08-30

Applicant: ILLUMINA, INC.

Inventor： Ali Agah ,  Aathavan Karunakaran ,  Tarun Khurana ,  Stanley Hong ,  Merek Siu ,  Arvin Emadi ,  Craig Ciesla

IPC: B01L3/00 ,  G11C13/02 ,  B01J19/00 ,  C12Q1/6874 ,  C12N15/09

CPC classification number: B01L3/502715 ,  B01J19/0046 ,  B01L3/502707 ,  B01L3/502738 ,  G11C13/02 ,  B01J2219/00711 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01L2200/026 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0655 ,  B01L2400/08 ,  C12N15/09 ,  C12Q1/6874

Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.