公开(公告)号：US12102998B2

公开(公告)日：2024-10-01

申请号：US17297219

申请日：2019-02-01

申请人： HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

发明人： Alexander Govyadinov ,  Viktor Shkolnikov

IPC分类号： B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B01L7/00 ,  B23Q17/24 ,  C08J3/075 ,  C08K3/16 ,  C08K3/22 ,  C08K3/32 ,  C12Q1/18 ,  G01N15/01 ,  G01N15/14 ,  G01N15/149 ,  G01N21/64 ,  G01N27/411 ,  G01N33/50

CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/1827 ,  B01L2400/0442 ,  G01N15/01 ,  G01N15/149 ,  G01N2015/1493

摘要： In one example in accordance with the present disclosure, a cell sorting device is described. The cell sorting device includes a microfluidic channel to serially transport individual cells from a volume of cells along a flow path. A sensor disposed in the microfluidic channel distinguishes between a cell to be analyzed and waste fluid. The cell sorting device includes at least two fluid transport devices disposed within the microfluidic channel. The at least two fluid transport devices include a cell ejector to, responsive to detection of a cell to be analyzed, eject the cell to be analyzed from the cell sorting device and a waste transport device to direct the waste fluid to a waste reservoir.

公开(公告)号：US20240319091A1

公开(公告)日：2024-09-26

申请号：US18579572

申请日：2022-07-18

申请人： REFEYN LTD

发明人： Matthias LANGHORST ,  Joshua BISHOP

IPC分类号： G01N21/45 ,  G01N15/0227 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434 ,  G01N33/543 ,  G03H1/32

CPC分类号： G01N21/45 ,  G01N15/0227 ,  G01N15/1433 ,  G01N15/1434 ,  G01N33/54373 ,  G03H1/32 ,  G01N2015/1021 ,  G01N2015/1027 ,  G01N2015/1454 ,  G01N2015/1493 ,  G03H2210/62 ,  G03H2222/43 ,  G03H2223/52

摘要： The present invention relates to an improved method for determining the identity of a biomolecule using interferometric light scattering apparatus, notably distinguishing between variants of biomolecules, such as viral serotypes and the like. Such a method has potential to simplify the testing for biological manufacture and for diagnosing disease, monitoring environmental issues and determination of contaminants.

公开(公告)号：US12078586B2

公开(公告)日：2024-09-03

申请号：US17433740

申请日：2020-01-16

申请人： SONY GROUP CORPORATION

发明人： Yoshiki Okamoto

IPC分类号： G01N15/1434 ,  G01N15/14 ,  G01N15/149 ,  G02B3/00 ,  G02B13/14

CPC分类号： G01N15/1434 ,  G02B3/0006 ,  G02B13/143 ,  G01N15/149 ,  G01N2015/1493

摘要： To provide a microparticle measurement technology capable of supporting excitation light in a wideband wavelength range.
The present technology provides a microparticle measurement device provided with a plurality of objective lenses for excitation light irradiation used for irradiating microparticles flowing through a flow path with excitation light, in which at least one of the objective lenses for excitation light irradiation is used for detecting scattered light emitted from the microparticles by the excitation light with which the microparticles are irradiated through another one of the objective lenses for excitation light irradiation.

公开(公告)号：US20240288356A1

公开(公告)日：2024-08-29

申请号：US18639375

申请日：2024-04-18

申请人： 1087 Systems, Inc.

发明人： Matthias Wagner ,  John Heanue

IPC分类号： G01N15/14 ,  B01L3/00 ,  B07C5/34 ,  C12N5/071 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1429 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/149 ,  G01N21/17 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/65 ,  G01N29/24

CPC分类号： G01N15/147 ,  B01L3/502761 ,  B07C5/34 ,  C12N5/0612 ,  G01N15/14 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/1702 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/65 ,  G01N29/2418 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0864 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1481 ,  G01N15/149 ,  G01N2015/1493 ,  G01N2015/1497 ,  G01N2021/653 ,  G01N2201/0612

摘要： This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

公开(公告)号：US20240288355A1

公开(公告)日：2024-08-29

申请号：US18572875

申请日：2021-06-24

申请人： LEICA MICROSYSTEMS CMS GMBH

发明人： Soeren ALSHEIMER

IPC分类号： G01N15/10 ,  G01N15/14 ,  G02B21/00

CPC分类号： G01N15/1012 ,  G01N15/14 ,  G01N15/1459 ,  G02B21/0076 ,  G02B21/008 ,  G01N2015/1014 ,  G01N2015/1493

摘要： A calibration object for calibrating an imaging system includes a discrete entity with a calibration pattern. The discrete entity is made of at least one transparent polymeric compound.

公开(公告)号：US12070725B2

公开(公告)日：2024-08-27

申请号：US18315911

申请日：2023-05-11

申请人： Optofluidics Inc.

发明人： Thomas Castner ,  Robert Hart ,  Colby Ashcroft ,  Brian DiPaolo ,  Nathan Wall

IPC分类号： B01D69/10 ,  G01N15/0227 ,  G01N15/14 ,  G06T7/00

CPC分类号： B01D69/106 ,  G01N15/0227 ,  G01N15/14 ,  G06T7/001 ,  G01N2015/1493 ,  G06T2207/10056

摘要： A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.

公开(公告)号：US12044606B2

公开(公告)日：2024-07-23

申请号：US17290675

申请日：2019-10-31

申请人： INESC TEC—INSTITUTO DE ENGENHARIA DE SISTEMAS E COMPUTADORES, TECNOLOGIA E CIÊNCIA

发明人： Joana Isabel Dos Santos Paiva ,  João Paulo Trigueiros Da Silva Cunha ,  Pedro Alberto Da Silva Jorge

IPC分类号： G01N15/0205 ,  G01N15/00 ,  G01N15/01 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1434

CPC分类号： G01N15/0211 ,  G01N15/1429 ,  G01N15/1434 ,  G01N2015/0053 ,  G01N15/01 ,  G01N2015/0238 ,  G01N2015/1006 ,  G01N2015/1488 ,  G01N2015/1493

摘要： Device and method for detecting dispersed extracellular vesicles in a liquid dispersion sample, said method using an electronic data processor for classifying the sample as having, or not having, extracellular vesicles present, the method comprising the use of the electronic data processor for pre-training a machine learning classifier with a plurality of extracellular vesicle liquid dispersion specimens comprising the steps of: emitting a laser modulated by a modulation frequency onto each specimen; capturing a temporal signal from laser light backscattered by each specimen for a plurality of temporal periods of a predetermined duration for each specimen; calculating specimen DCT or Wavelet transform coefficients from the captured signal for each of the temporal periods; using the calculated coefficients to pre-train the machine learning classifier; wherein the method further comprises the steps of: using a laser emitter having a focusing optical system coupled to the emitter to emit a laser modulated by a modulation frequency onto the sample; using a light receiver to capture a signal from laser light backscattered by the sample for a plurality of temporal periods of a predetermined duration; calculating sample DCT or Wavelet transform coefficients from the captured signal for each of the temporal periods; using the pre-trained machine learning classifier to classify the calculated sample coefficients as having, or not having, extracellular vesicles present.

公开(公告)号：US20240192119A1

公开(公告)日：2024-06-13

申请号：US18076503

申请日：2022-12-07

申请人： THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITY

发明人： Parisa A. ARIYA ,  Devendra PAL

IPC分类号： G01N15/14 ,  G03H1/00 ,  G03H1/22

CPC分类号： G01N15/1434 ,  G03H1/0005 ,  G03H1/2205 ,  G01N2015/0065 ,  G01N2015/1493 ,  G01N2015/1497

摘要： A real-time, in situ and automated technology and method for dynamic observation of airborne viruses has been developed, and its application for airborne viruses, using a model virus, MS2 bacteriophage, and SARS-COV-1 RNA, has been demonstrated. There is described a method of performing measurements using a digital in-line holographic microscope (DIHM). The method generally has: propagating a light beam through a pinhole, across a medium including particles, and to a sensor; acquiring, with the sensor, a plurality of holograms, the holograms including scattering information of the particles; and determining, from a reconstruction of the holograms, at least one of shape, size, intensity and phase of the particles from the scattering information of said particles.

公开(公告)号：US11988590B2

公开(公告)日：2024-05-21

申请号：US17594507

申请日：2020-04-27

申请人： CytoChip Inc.

发明人： Wendian Shi

IPC分类号： G01N15/10 ,  G01N15/0205 ,  G01N15/14 ,  G01N33/49 ,  G01N15/01

CPC分类号： G01N15/1012 ,  G01N15/0205 ,  G01N15/1459 ,  G01N33/4915 ,  G01N15/01 ,  G01N2015/1493

摘要： Methods and devices for correction in particle size measurement are disclosed. In some embodiments, a method includes the following steps: (1) measuring a signal from a target particle and a reference particle in a cartridge device; (2) analyzing the measured signal to obtain signal information of the target particle and signal information of the reference particle; and (3) determining size information of the target particle by correcting the signal information of the target particle with the signal information of the reference particle. In other embodiments, a device includes a cartridge and an analyzer. The analyzer is configured to receive the cartridge into the analyzer, measure a signal from the target particle and the reference particle, analyze the measured signal to obtain signal information, and determine size information of the target particle.

公开(公告)号：US20240159649A1

公开(公告)日：2024-05-16

申请号：US18503568

申请日：2023-11-07

申请人： KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Yong Bok LEE ,  Wonil KWAK ,  YunSeok HA ,  Byul AN

IPC分类号： G01N15/14 ,  G06T7/246 ,  G06T7/285 ,  G06T7/292 ,  G06T7/62 ,  G06V20/60

CPC分类号： G01N15/1463 ,  G01N15/1459 ,  G06T7/248 ,  G06T7/285 ,  G06T7/292 ,  G06T7/62 ,  G06V20/60 ,  G01N2015/1075 ,  G01N2015/1477 ,  G01N2015/1493

摘要： Provided is a fluid monitoring method including photographing a particle in a flowing fluid using a first camera, photographing the particle using a second camera spaced apart from the first camera, and analyzing the particle using images captured by each of the first camera and the second camera, in which the analyzing of the particle includes calculating a volume of the particle, calculating a velocity of the particle moving in the fluid, calculating a density of the particle using the volume and the velocity, and identifying a type of the particle using the density.