公开(公告)号：US12083515B2

公开(公告)日：2024-09-10

申请号：US18118277

申请日：2023-03-07

Applicant: FLORIDA ATLANTIC UNIVERSITY BOARD OF TRUSTEES

Inventor： E Du ,  Darryl Dieujuste ,  Jia Liu ,  Yuhao Qiang

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/1031 ,  G01N15/14 ,  G01N15/1429 ,  G01N33/49 ,  G01N15/01

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  G01N15/1031 ,  G01N33/4915 ,  B01L2300/025 ,  B01L2300/06 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2300/16 ,  G01N2015/012 ,  G01N2015/1006 ,  G01N15/1425 ,  G01N15/1431 ,  G01N15/1484

Abstract: An exemplary mobile impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. The mobile cytometer may be controlled by a computer (e.g., smartphone) application. Calibration of the portable device may be performed using a component of known impedance value. With the developed portable flow cytometer, analysis may be performed on two sickle cell samples and a healthy cell sample. The acquired results may subsequently be analyzed to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals may be observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.

公开(公告)号：US12044614B2

公开(公告)日：2024-07-23

申请号：US17589507

申请日：2022-01-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  G01N15/00 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1484 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1433 ,  G01N35/00029 ,  G01N35/1011 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/021 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G01N2035/1039

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US12031899B2

公开(公告)日：2024-07-09

申请号：US17102388

申请日：2020-11-23

Applicant: IMEC VZW

Inventor： Dries Vercruysse

IPC: G01N15/1434 ,  F21V8/00 ,  G01N15/14 ,  G01N21/64 ,  G01N21/65 ,  G02B6/124 ,  G02B6/30 ,  G01N15/10 ,  G01N21/03

CPC classification number: G01N15/1436 ,  G01N15/1434 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/65 ,  G02B6/0036 ,  G02B6/124 ,  G02B6/30 ,  G01N2015/1006 ,  G01N2021/0346 ,  G01N2021/6463 ,  G01N2021/6482

Abstract: A radiation carrier for carrying at least a radiation beam has, on a surface thereof, at least one excitation grating, for directing at least an excitation radiation beam directionally out of the radiation carrier, thereby illuminating a region of interest; and at least one structure for redirecting emission radiation emanating from the region of interest. Further a sensor is provided comprising at least one such radiation carrier and at least one detector, the structure being adapted for redirecting radiation from the region of interest into the at least one detector.

公开(公告)号：US20240216913A1

公开(公告)日：2024-07-04

申请号：US18562149

申请日：2022-05-26

Applicant: Menarini Silicon Biosystems S.p.A.

Inventor： Gianni Medoro ,  Giulio Signorini

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G16B40/10

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  G01N15/1433 ,  G01N15/1484 ,  G16B40/10 ,  B01L2200/0652 ,  B01L2300/0663 ,  B01L2400/0487 ,  G01N2015/1006 ,  G01N2015/1027 ,  G01N2015/1497

Abstract: Method and microfluidic system (1) for the manipulation of particles; a detection device (7) acquires images of a specific particle (5) in a first position (IP) and in a second position (IIP); the difference is made between the two images in order to obtain a derived image in which the contours and the morphological characteristics of the specific particle (5) are more evident; in this manner, the type and the position of the particles can be identified more clearly, continuously and in a time-saving manner.

公开(公告)号：US20240198338A1

公开(公告)日：2024-06-20

申请号：US18595920

申请日：2024-03-05

Applicant: The General Hospital Corporation

Inventor： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC: B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/01 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N15/149

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N15/01 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N15/149 ,  G01N2015/1493

Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：US12005453B2

公开(公告)日：2024-06-11

申请号：US16933149

申请日：2020-07-20

Applicant: Owl biomedical, Inc.

Inventor： Mehran Hoonejani ,  John Harley

IPC: B01L3/00 ,  F16K99/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N21/64 ,  G01N15/149

CPC classification number: B01L3/502761 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  F16K99/0046 ,  G01N15/1404 ,  G01N15/1484 ,  G01N21/6402 ,  G01N21/6428 ,  G01N21/6486 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2400/0622 ,  B01L2400/0633 ,  G01N15/149 ,  G01N2021/6439 ,  G01N2201/06113

Abstract: A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which has a sample inlet channel, output channels, and a movable member formed on a substrate. The device may be used to separate a target particle from non-target material in a sample stream. In order to improve the sorter speed, accuracy or yield, the particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the sample inlet channel. The particle manipulation device may have two separate sort output channels, wherein the sort channel used depends on the characteristics of the sort pulse delivered to the micromechanical particle manipulation device.

公开(公告)号：US20240133876A1

公开(公告)日：2024-04-25

申请号：US18382849

申请日：2023-10-22

Applicant: University of Georgia Research Foundation, Inc.

Inventor： Leidong Mao ,  Yang Liu

IPC: G01N33/543 ,  B01L3/00 ,  G01N15/14 ,  G01N15/1404

CPC classification number: G01N33/54366 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  G01N15/1404 ,  G01N15/1484 ,  B01L2200/0647 ,  B01L2400/043 ,  G01N2015/1006

Abstract: The present disclosure provides devices, kits, and methods for focusing/enriching and/or separating/sorting submicron size particles, including biological entities such as exosomes and other submicron size extracellular vesicles. Devices, kits, and methods of the present disclosure utilize ferrohydrodynamic manipulation to focus populations of submicron particles into a stream for enrichment and/or further sort various sub-populations of submicron particles based on size differences.

公开(公告)号：US11946855B2

公开(公告)日：2024-04-02

申请号：US18195084

申请日：2023-05-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  B01L3/00 ,  B01L3/02 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/10 ,  G01N33/487 ,  G06V20/69 ,  G01N15/02 ,  G01N35/00

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  G01N1/4077 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G06V20/69

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US11944971B2

公开(公告)日：2024-04-02

申请号：US17245770

申请日：2021-04-30

Applicant: The General Hospital Corporation

Inventor： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC: B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：US11898954B2

公开(公告)日：2024-02-13

申请号：US16449370

申请日：2019-06-22

Applicant: owl biomedical, inc.

Inventor： John S. Foster ,  Mark A. Naivar ,  Kevin E. Shields ,  Daryl W. Grummitt ,  Lily Li ,  Yareeve Zemel

IPC: G01N15/10 ,  G01N15/14 ,  B01L3/00 ,  B07C5/342

CPC classification number: G01N15/1463 ,  B01L3/502761 ,  B07C5/3425 ,  G01N15/1404 ,  G01N15/1425 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0454 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2015/1413

Abstract: A MEMS-based particle manipulation system which uses a particle manipulation stage and optical confirmation of the manipulation. The optical confirmation may be camera-based, and may be used to assess the effectiveness or accuracy of the particle manipulation stage. In one exemplary embodiment, the particle manipulation stage is a microfabricated, fluid valve, which sorts a target particle from non-target particles in a fluid stream. The optical confirmation stage is disposed in the microfabricated fluid channels at the input and output of the microfabricated sorting valve. Deep learning techniques are brought to bear on the camera output to increase speed, accuracy and reliability.