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公开(公告)号：US12105010B2
公开(公告)日：2024-10-01
申请号：US18373487
申请日：2023-09-27
发明人： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC分类号： G01N15/14 , G01N15/1429 , G01N15/1434 , G01N15/149 , G01N15/1404
CPC分类号： G01N15/1459 , G01N15/1425 , G01N15/1429 , G01N15/1434 , G01N2015/1402 , G01N2015/142 , G01N2015/1486 , G01N15/149
摘要： Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US12083516B2
公开(公告)日：2024-09-10
申请号：US18118279
申请日：2023-03-07
发明人： E Du , Darryl Dieujuste , Jia Liu , Yuhao Qiang
IPC分类号： B01L3/00 , G01N15/10 , G01N15/1031 , G01N15/14 , G01N15/1429 , G01N33/49 , G01N15/01
CPC分类号： B01L3/502715 , B01L3/502761 , G01N15/1031 , G01N33/4915 , B01L2300/025 , B01L2300/06 , B01L2300/0645 , B01L2300/0809 , B01L2300/16 , G01N2015/012 , G01N2015/1006 , G01N15/1425 , G01N15/1431 , G01N15/1484
摘要： An exemplary mobile impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. The mobile cytometer may be controlled by a computer (e.g., smartphone) application. Calibration of the portable device may be performed using a component of known impedance value. With the developed portable flow cytometer, analysis may be performed on two sickle cell samples and a healthy cell sample. The acquired results may subsequently be analyzed to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals may be observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.
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公开(公告)号：US12038372B2
公开(公告)日：2024-07-16
申请号：US18198478
申请日：2023-05-17
发明人： Eric D. Diebold , Keegan Owsley , Jonathan Lin
IPC分类号： G01N15/1434 , G01N15/14 , G01N15/1429 , G01N15/1433 , G01N21/64 , G02B21/06 , G01N15/10
CPC分类号： G01N15/1434 , G01N15/14 , G01N15/1425 , G01N15/1429 , G01N15/1433 , G01N15/147 , G01N21/645 , G02B21/06 , G01N2015/1006 , G01N2015/1447 , G01N2015/1454 , G01N2021/6482
摘要： Aspects of the disclosure include a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) with a top-hat-shaped excitation beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion to generate an image of the sample from the detected fluorescence. In another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency (e.g., a horizontal extent), which is generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency and detected fluorescence is used to analyze the sample.
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公开(公告)号：US12019005B2
公开(公告)日：2024-06-25
申请号：US17310813
申请日：2020-02-14
申请人： NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE , HAMAMATSU PHOTONICS K.K.
IPC分类号： G01N15/14 , G02B21/36 , G01N15/01 , G01N15/1433 , G01N15/1434
CPC分类号： G01N15/1425 , G02B21/361 , G01N2015/012 , G01N2015/016 , G01N15/1433 , G01N2015/144
摘要： A cell observation system observes a cell moving in a flow path with a fluid, and includes a first imaging apparatus, a second imaging apparatus, and a control device. The first imaging apparatus includes a first optical system and a first imaging element, and captures an image of the cell at a first position in a moving direction. The second imaging apparatus includes a second optical system, in which a focus is adjusted based on a focus adjustment signal, and a second imaging element, and captures an image of the cell at a second position downstream of the first position. The control device obtains a passing position of the cell in a cross section of the flow path based on the image obtained by the first imaging element, generates the focus adjustment signal, and provides the signal to the second optical system.
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公开(公告)号：US11808687B2
公开(公告)日：2023-11-07
申请号：US17359032
申请日：2021-06-25
发明人： Andrew Homyk , Saurabh Vyawahare
IPC分类号： G01N15/14
CPC分类号： G01N15/1404 , G01N15/1425 , G01N15/1484 , G01N2015/149
摘要： Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles can have a refractive index substantially different from that of the channel and the medium, such that the particles interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be used for identifying, tracking, and sorting the particles.
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公开(公告)号：US20230333093A1
公开(公告)日：2023-10-19
申请号：US18134862
申请日：2023-04-14
发明人： Wenbo ZHENG , Bo YE , Donglan YAO , Huan QI , Yi YE
CPC分类号： G01N33/5094 , G01N15/1459 , G01N33/49 , G01N15/1425 , G01N2015/0084
摘要： A sample analyzer includes a sample preparation device, an impedance testing device, an optical testing device, and a controller. The controller is configured to control the impedance testing device to test a first test sample to obtain a first platelet counting result for a test blood sample based on electronic information of the first test sample; control the optical testing device to test a second test sample to obtain a second platelet counting result for the test blood sample based only on optical information of the second test sample or based on both the electronic information of the first test sample and the optical information of the second test sample; determine whether the first platelet counting result is unreliable due to abnormality of the test blood sample; and output the first and/or the second platelet counting result according to the result of the determination.
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公开(公告)号：US20230288311A1
公开(公告)日：2023-09-14
申请号：US18198478
申请日：2023-05-17
发明人： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC分类号： G01N15/1434 , G01N15/14 , G01N15/1425 , G01N15/147 , G01N15/1475 , G01N15/1429 , G01N21/645 , G02B21/06 , G01N2015/1447 , G01N2015/1454 , G01N2015/1006
摘要： In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US20230243736A1
公开(公告)日：2023-08-03
申请号：US18130571
申请日：2023-04-04
发明人： Jonathan Lin , Joseph T. Trotter , Keegan Owsley
CPC分类号： G01N15/1459 , G16C20/20 , G01N15/1425 , G01N15/1434 , B07C5/3425 , B07C5/361 , G06V20/695 , G06V20/698 , G06V10/87 , G01N2015/149
摘要： A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号：US11686663B2
公开(公告)日：2023-06-27
申请号：US17225567
申请日：2021-04-08
CPC分类号： G01N15/1429 , B07C5/3425 , G01N15/1425 , G01N2015/1006 , G01N2015/1081
摘要： Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods. Some such embodiments include receiving, from a particle analyzer, measurements for a first portion of particles associated with an experiment. Some embodiments also include generating a tree representing groups of related particles based at least in part on the measurements, wherein the tree includes at least three groups. Some embodiments also include generating a measure of relatedness between a first group and a second group of the tree based at least in part on the measurements. Some embodiments also include and configuring the particle analyzer to classify a subsequent particle associated with the experiment with the first group real-time, wherein the subsequent particle is not included in the first portion of particles. Some embodiments also include sorting the subsequent particle.
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公开(公告)号：US20180292304A1
公开(公告)日：2018-10-11
申请号：US15919012
申请日：2018-03-12
申请人： SONY CORPORATION
发明人： Fumitaka Otsuka , Michael Zordan , Akiko Tsuji , Yuya Suzuki , Masashi Kimoto
IPC分类号： G01N15/14
CPC分类号： G01N15/1436 , G01N15/1404 , G01N15/1425 , G01N2015/1406 , G01N2015/144 , G01N2015/149
摘要： A microparticle sorting apparatus includes a detection unit which detects microparticles flowing through a flow path; an imaging device which images a droplet containing the microparticles which is discharged from an orifice provided on an edge portion of the flow path; a charge unit which applies a charge to the droplets; and a control unit which determines a delay time as from a time that the microparticles are detected by the detection unit to the time at which the sum of intensity of an image region imaged by the imaging device reaches a maximum, making it possible for the charge unit to apply a charge to the microparticles once the delay time has lapsed after the microparticles are detected by the detection unit.
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