公开(公告)号：US12072330B2

公开(公告)日：2024-08-27

申请号：US16915851

申请日：2020-06-29

申请人： SYSMEX CORPORATION

发明人： Saori Otsuka ,  Kazuki Hatcho ,  Toshihiro Mizukami ,  Shinichiro Oguni

IPC分类号： G01N33/50 ,  G01N15/14 ,  G01N15/1429 ,  G01N21/49 ,  G01N21/64 ,  G01N15/01

CPC分类号： G01N33/5094 ,  G01N15/1429 ,  G01N15/147 ,  G01N21/49 ,  G01N21/6428 ,  G01N2015/011 ,  G01N2015/016

摘要： The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The present invention also provides a regent kit and reagent for classifying leukocyte which are used for classifying and counting leukocytes in biological samples.

公开(公告)号：US20240210297A1

公开(公告)日：2024-06-27

申请号：US18564650

申请日：2022-05-16

申请人： Beckman Coulter, Inc.

发明人： Jozef SOFKA

IPC分类号： G01N15/0227 ,  G01N15/01 ,  G01N15/14 ,  G01N15/1434

CPC分类号： G01N15/0227 ,  G01N15/01 ,  G01N15/1434 ,  G01N15/147 ,  G01N2015/1445

摘要： Techniques for oblique illumination of samples in microscopy imaging are presented. The sample may be obliquely illuminated by directing light from a light emitter toward a sample at an oblique angle, rather than directly along the optical axis of an imaging device. The oblique angle may be created using an aperture mask with an aperture that is not coaxial with the optical axis of the imaging device. The oblique angle may also be created using a light emitter and collector lens that are not coaxial with the optical axis of the imaging device, such that the condenser lens directs the light toward the sample. Images obtained using the oblique illumination provide improved contrast of translucent particles and may have an embossed, three-dimensional appearance.

公开(公告)号：US20240085399A1

公开(公告)日：2024-03-14

申请号：US18508899

申请日：2023-11-14

申请人： Leuko Labs, Inc.

发明人： Carlos Castro Gonzalez ,  Ian Butterworth ,  Aurelien Bourquard ,  Alvaro Sanchez Ferro

IPC分类号： G01N33/49 ,  G01N15/14 ,  G01N21/31 ,  G06F18/214 ,  G06T7/00 ,  G06T7/11 ,  G06T7/38 ,  G06V10/56 ,  G06V10/764 ,  G06V10/82 ,  G06V20/69 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70

CPC分类号： G01N33/49 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/31 ,  G06F18/2148 ,  G06T7/0016 ,  G06T7/11 ,  G06T7/38 ,  G06V10/56 ,  G06V10/764 ,  G06V10/82 ,  G06V20/695 ,  G06V20/698 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70 ,  G01N2015/0073 ,  G06T2207/10016 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30101 ,  G06T2207/30242

摘要： In one aspect, a method to detect white blood cells and/or white blood cell subtypes from non-invasive capillary videos is featured. The method includes acquiring a first plurality of images of a region of interest including one or more capillaries of a predetermined area of a human subject from non-invasive capillary videos captured with an optical device, processing the first plurality of images to determine one or more optical absorption gaps located in said capillary, and annotating the first plurality of images with an indication of any optical absorption gap detected in the first plurality of images. The method also includes acquiring a second plurality of images of the same region of interest of the same capillary with an advanced optical device capable of resolving cellular structure of white blood cells and white blood cell subtypes and spatiotemporally annotating the second plurality of images with an indication of any white blood cell detected and/or a subtype of any white blood cell detected in the second plurality of images. The method also includes inputting the first plurality of images and annotated information from the first plurality of images and annotated information from the spatiotemporally annotated second plurality of images into a machine learning subsystem configured to determine a presence of white blood cells and/or the subtype of any white blood cells present in the one or more optical absorption gaps in the first plurality of images.

公开(公告)号：US11860154B2

公开(公告)日：2024-01-02

申请号：US17331893

申请日：2021-05-27

申请人： Leuko Labs, Inc.

发明人： Carlos Castro Gonzalez ,  Ian Butterworth ,  Aurelien Bourquard ,  Alvaro Sanchez Ferro

IPC分类号： G01N33/49 ,  G06T7/00 ,  G06T7/11 ,  G06T7/38 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70 ,  G01N21/31 ,  G06V20/69 ,  G06F18/214 ,  G06V10/764 ,  G06V10/82 ,  G06V10/56 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N33/49 ,  G01N15/147 ,  G01N15/1429 ,  G01N15/1475 ,  G01N21/31 ,  G06F18/2148 ,  G06T7/0016 ,  G06T7/11 ,  G06T7/38 ,  G06V10/56 ,  G06V10/764 ,  G06V10/82 ,  G06V20/695 ,  G06V20/698 ,  G16H30/20 ,  G16H30/40 ,  G16H50/20 ,  G16H50/70 ,  G01N2015/008 ,  G01N2015/0073 ,  G01N2015/1006 ,  G01N2015/1443 ,  G01N2015/1486 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30101 ,  G06T2207/30242 ,  G06V2201/03

摘要： In one aspect, a method to detect white blood cells and/or white blood cell subtypes from non-invasive capillary videos is featured. The method includes acquiring a first plurality of images of a region of interest including one or more capillaries of a predetermined area of a human subject from non-invasive capillary videos captured with an optical device, processing the first plurality of images to determine one or more optical absorption gaps located in said capillary, and annotating the first plurality of images with an indication of any optical absorption gap detected in the first plurality of images. The method also includes acquiring a second plurality of images of the same region of interest of the same capillary with an advanced optical device capable of resolving cellular structure of white blood cells and white blood cell subtypes and spatiotemporally annotating the second plurality of images with an indication of any white blood cell detected and/or a subtype of any white blood cell detected in the second plurality of images. The method also includes inputting the first plurality of images and annotated information from the first plurality of images and annotated information from the spatiotemporally annotated second plurality of images into a machine learning subsystem configured to determine a presence of white blood cells and/or the subtype of any white blood cells present in the one or more optical absorption gaps in the first plurality of images.

公开(公告)号：US20230349808A1

公开(公告)日：2023-11-02

申请号：US18350336

申请日：2023-07-11

申请人： Kinetic River Corp.

发明人： Giacomo Vacca ,  Ralph Jimenez

IPC分类号： G01N15/14

CPC分类号： G01N15/14 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2015/149

摘要： Described herein are apparatuses and methods for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and quantifying a relative contribution of each component to the supercurve.

公开(公告)号：US11774343B2

公开(公告)日：2023-10-03

申请号：US17386192

申请日：2021-07-27

申请人： Becton, Dickinson and Company

发明人： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

IPC分类号： G01N15/14 ,  G01N21/64 ,  G01N15/10

CPC分类号： G01N15/1434 ,  G01N15/147 ,  G01N15/1429 ,  G01N21/6402 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1447 ,  G01N2021/6439

摘要： In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

公开(公告)号：US20230258554A1

公开(公告)日：2023-08-17

申请号：US18006205

申请日：2021-07-21

申请人： RAMOT AT TEL-AVIV UNIVERSITY LTD. ,  FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E. V.

发明人： Natan Tzvi SHAKED ,  Itay BARNEA ,  Matan DUDAIE ,  Noga NISSIM ,  Michael KIRSCHBAUM ,  Marten Tobias GERLING

IPC分类号： G01N15/14 ,  B01L3/00

CPC分类号： G01N15/1475 ,  B01L3/502761 ,  B01L3/502715 ,  G01N15/147 ,  B01L2400/0424 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2200/0652 ,  G01N2015/1006

摘要： The present invention relates to an automatic real-time label-free holography-activated sorting of the cell's technique. The technique provides high-discriminative power on the level of the individual cell. The technique includes rapid automated cell processing during cell visualization and flow, with high discriminative power on the level of the individual cell. The technique may be useful in detection of cancer and to identify different stages of oncogenesis.

公开(公告)号：US11718826B2

公开(公告)日：2023-08-08

申请号：US17406967

申请日：2021-08-19

申请人： Inguran, LLC

发明人： Gary Durack ,  Jeffrey D. Wallace ,  Gary P. Vandre ,  Lon A. Westfall ,  Jeremy T. Hatcher ,  Niraj V. Nayak

IPC分类号： G01N33/48 ,  C12N5/076 ,  C12Q1/02 ,  G01N15/14 ,  C12N5/071 ,  A01N1/02 ,  C12Q3/00 ,  G01N33/50 ,  G01N21/64 ,  C12N5/07 ,  G01N21/63 ,  G01N15/00 ,  G01N15/10

CPC分类号： C12N5/0612 ,  A01N1/0284 ,  C12N5/06 ,  C12N5/061 ,  C12Q1/02 ,  C12Q3/00 ,  G01N15/1404 ,  G01N15/147 ,  G01N15/1459 ,  G01N15/1468 ,  G01N21/6428 ,  G01N33/48 ,  G01N33/5005 ,  G01N21/63 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/149 ,  G01N2015/1415 ,  G01N2021/6439 ,  Y10T436/10 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/255 ,  Y10T436/2525 ,  Y10T436/2575 ,  Y10T436/25375

摘要： A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

公开(公告)号：US11668641B2

公开(公告)日：2023-06-06

申请号：US17326312

申请日：2021-05-20

申请人： The Regents of the University of California

发明人： Yu-Hwa Lo ,  Yuanyuan Han ,  Yi Gu ,  Ce Zhang

IPC分类号： G06K9/00 ,  G01N15/14 ,  G01J3/51 ,  G01J3/02 ,  G01J3/36 ,  G01J3/46 ,  G01J3/44 ,  G01N15/10

CPC分类号： G01N15/1475 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/36 ,  G01J3/4406 ,  G01J3/46 ,  G01J3/51 ,  G01N15/147 ,  G01N15/1429 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/149 ,  G01N2015/1497

摘要： Disclosed are systems, devices and methods for imaging and image-based sorting of particles in a flow system, including cells in a flow cytometer. In some aspects, a system includes a particle flow device to flow particles through a channel, an imaging system to obtain image data of a particle during flow through the channel, a processing unit to determine a property associated with the particle and to produce a control command for sorting the particle based on sorting criteria associated with particle properties, and an actuator to direct the particle into one of a plurality of output paths of the particle flow device in real-time.

公开(公告)号：US20230152203A1

公开(公告)日：2023-05-18

申请号：US17984116

申请日：2022-11-09

申请人： Iris International, Inc.

发明人： Thomas H. Adams ,  Bart J. Wanders ,  John Roche ,  Harvey L. Kasdan

IPC分类号： G01N15/14 ,  G01N33/50 ,  G01N15/06 ,  G01N21/53 ,  G02B7/36 ,  G01N33/80 ,  G01N1/30 ,  G01N33/49

CPC分类号： G01N15/1404 ,  G01N33/5094 ,  G01N15/06 ,  G01N15/1468 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/53 ,  G01N15/1459 ,  G01N15/1429 ,  G01N15/1436 ,  G02B7/36 ,  G01N33/5091 ,  G01N33/80 ,  G01N1/30 ,  G01N33/49 ,  G01N33/4915 ,  G01N2015/1452 ,  G01N2015/0065

摘要： For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.