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公开(公告)号:US12121547B2
公开(公告)日:2024-10-22
申请号:US16747406
申请日:2020-01-20
申请人: Inguran, LLC
发明人: Hein van der Steen , Gregg Bevier
CPC分类号: A61K35/52 , A61B1/044 , A61B1/3132 , A61B6/12 , A61B6/485 , A61D19/027
摘要: The invention relates to methods of increasing genetic merit of swine by establishing a plurality of mating subtypes for a line of swine, and determining a percentage of progeny that are male for each of the mating subtypes, or a percentage of progeny that are female for each of the mating subtypes, that would result, relative to a control, in an increase in genetic merit in the line; the invention further relates to sorting a sperm cell sample from a male swine in one of the mating subtypes into one or more subpopulations of sperm cells, wherein a majority of sperm cells in a subpopulation of sperm cells bear X chromosomes or Y chromosomes, and inseminating one or more female swine in the one of the mating subtypes with the subpopulation of sperm cells to achieve the percentage of progeny that are male, or the percentage of progeny that are female, determined to increase genetic merit relative to the control.
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公开(公告)号:US12084678B2
公开(公告)日:2024-09-10
申请号:US18109536
申请日:2023-02-14
申请人: INGURAN, LLC
IPC分类号: G01N15/14 , C12N5/071 , G01N15/149
CPC分类号: C12N5/0612 , G01N15/1459 , G01N15/1484 , G01N15/149
摘要: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.
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公开(公告)号:US20230384201A1
公开(公告)日:2023-11-30
申请号:US18140288
申请日:2023-04-27
申请人: Inguran, LLC
IPC分类号: G01N15/14 , G01N33/487
CPC分类号: G01N15/1404 , G01N15/14 , G01N15/1434 , G01N33/487 , G01N2015/1409 , Y10T29/49401 , G01N2015/149
摘要: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbilng a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.
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公开(公告)号:US20230303970A1
公开(公告)日:2023-09-28
申请号:US18134451
申请日:2023-04-13
申请人: Inguran, LLC
CPC分类号: C12N5/061 , G01N33/582 , C12N13/00 , G01N1/30
摘要: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.
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公开(公告)号:US11653635B2
公开(公告)日:2023-05-23
申请号:US16708007
申请日:2019-12-09
申请人: Inguran, LLC
发明人: Juan Moreno , Gregg Bevier , John Dobrinsky
IPC分类号: A01K67/00 , A01K67/02 , A61D19/02 , A61B34/20 , G16B20/40 , A61B1/04 , A61B1/015 , A61B1/303 , A61B1/313 , A61B8/08 , A61B10/00 , A61M31/00 , G16B20/00
CPC分类号: A01K67/02 , A61B1/015 , A61B1/04 , A61B1/303 , A61B1/313 , A61B8/08 , A61B10/0012 , A61B34/20 , A61D19/027 , A61M31/002 , G16B20/40 , A01K2227/108 , A61B2034/2057 , G16B20/00
摘要: The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.
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公开(公告)号:US11591566B2
公开(公告)日:2023-02-28
申请号:US16577872
申请日:2019-09-20
申请人: INGURAN, LLC
摘要: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.
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公开(公告)号:US20220381673A1
公开(公告)日:2022-12-01
申请号:US17882787
申请日:2022-08-08
申请人: INGURAN, LLC
发明人: Alex Hegyi , Peter Kiesel , Kenneth M. Evans
摘要: An optical arrangement receives output light emanating from an object disposed within a fluid column that crosses an optical refraction boundary of the fluid column between the object and the optical arrangement. The optical arrangement modifies the output light such that the modified output light has an intensity that is more uniform than the output light.
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公开(公告)号:US11326146B2
公开(公告)日:2022-05-10
申请号:US14947912
申请日:2015-11-20
申请人: Inguran, LLC
摘要: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
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公开(公告)号:US20220025443A1
公开(公告)日:2022-01-27
申请号:US17384390
申请日:2021-07-23
申请人: Inguran, LLC
发明人: Walid Korani , Nader Deeb , Alan Mileham , Charles Michael Cowan
IPC分类号: C12Q1/6816 , C12Q1/6806 , C12Q1/6827 , G16B30/10
摘要: The invention includes a method of screening a biological sample for contamination by sequencing a first biological sample at a first depth to generate a first nucleotide sequence; determining map coordinates for one or more homozygous loci in the first nucleotide sequence; sequencing a second biological sample at a second depth to generate a second nucleotide sequence, wherein the second depth is lower than the first depth; determining loci in the second nucleotide sequence at the determined map coordinates; and comparing the one or more homozygous loci in the first nucleotide sequence with the determined loci in the second nucleotide sequence.
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公开(公告)号:US11105744B2
公开(公告)日:2021-08-31
申请号:US16039629
申请日:2018-07-19
申请人: Inguran, LLC
IPC分类号: G01N21/64 , G01N15/14 , C12N5/071 , C12Q1/6879 , G02B27/09 , G01N15/10 , H01S3/0941
摘要: This disclosure pertains to analytical instruments and related methods incorporating beam shaping optics for differentiating very bright and closely related signals over a wide range of operating conditions with an improved and uniform performance.
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