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公开(公告)号：US20240353309A1
公开(公告)日：2024-10-24
申请号：US18734985
申请日：2024-06-05
发明人： Eric D. Diebold , Keegan Owsley , Jonathan Lin
IPC分类号： G01N15/1434 , G01N15/10 , G01N15/14 , G01N15/1429 , G01N15/1433 , G01N21/64 , G02B21/06
CPC分类号： G01N15/1434 , G01N15/14 , G01N15/1425 , G01N15/1429 , G01N15/1433 , G01N15/147 , G01N21/645 , G02B21/06 , G01N2015/1006 , G01N2015/1447 , G01N2015/1454 , G01N2021/6482
摘要： In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US11808690B2
公开(公告)日：2023-11-07
申请号：US18095908
申请日：2023-01-11
发明人： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC分类号： G01N15/14
CPC分类号： G01N15/1459 , G01N15/1425 , G01N15/1429 , G01N15/1434 , G01N2015/1402 , G01N2015/142 , G01N2015/149 , G01N2015/1486
摘要： Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US11698334B2
公开(公告)日：2023-07-11
申请号：US17003605
申请日：2020-08-26
发明人： Eric D. Diebold , Keegan Owsley , Matthew Bahr
CPC分类号： G01N15/1434 , G01N15/1459 , G02F1/33 , H01S3/0071 , G01N2015/1006 , G01N2015/145 , G02F2203/18 , G02F2203/24
摘要： Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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公开(公告)号：US20230144214A1
公开(公告)日：2023-05-11
申请号：US18095908
申请日：2023-01-11
发明人： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC分类号： G01N15/14
CPC分类号： G01N15/1459 , G01N15/1434 , G01N15/1425 , G01N15/1429 , G01N2015/1486 , G01N2015/1402 , G01N2015/142 , G01N2015/149
摘要： Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US20230062339A1
公开(公告)日：2023-03-02
申请号：US17975243
申请日：2022-10-27
发明人： Peter Mage , Keegan Owsley
摘要： Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.
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公开(公告)号：US20230022846A1
公开(公告)日：2023-01-26
申请号：US17849274
申请日：2022-06-24
发明人： Jizuo Zou , Keegan Owsley , Matthew Bahr
IPC分类号： G01N15/14
摘要： Aspects of the present disclosure include methods for determining a photodetector gain correction factor for application to flow cytometer data. Methods according to certain embodiments include detecting light with a light detection system across a horizontal axis of a flow stream, generating data signals in a photodetector channel (e.g., an imaging photodetector channel) of the light detection system at a plurality of positions across the flow stream and calculating a detector gain correction factor for each position across the flow stream in response to the generated data signals. Methods also include applying a detector gain correction factor to data signals from a photodetector channel (e.g., non-imaging photodetector channels) to generate adjusted signal intensities. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector (e.g., an imaging photodetector) for practicing the subject methods are also described. Non-transitory computer readable storage medium and integrated circuits (e.g., FPGAs) are also provided.
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7.发明申请公开(公告)号：US20220091017A1
公开(公告)日：2022-03-24
申请号：US17403105
申请日：2021-08-16
发明人： Peter Mage , Lingjie Wei , Peter Johnson , David A. Roberts , Keegan Owsley
IPC分类号： G01N15/14
摘要： Aspects of the present disclosure include methods for determining baseline noise of a photodetector (e.g., in a light detection system of a particle analyzer). Methods according to certain embodiments include irradiating a sample having particles in a flow stream, detecting light with the photodetector from the irradiated flow stream, generating data signals from the detected light and calculating a moving average mean squared error of the generated data signals to determine the baseline of the photodetector. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium are also provided.
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公开(公告)号：US11275026B2
公开(公告)日：2022-03-15
申请号：US16861724
申请日：2020-04-29
发明人： Matthew Bahr , Eric D. Diebold , Majid Mehrpouyan , Keegan Owsley
摘要： Aspects of the present disclosure include methods for phase correcting signals from a light detection system (e.g., in a flow cytometer). Methods according to certain embodiments include detecting light from a sample having particles in a flow stream with a light detection system that includes a brightfield photodetector configured to generate a brightfield data signal and a fluorescence detector configured to generate a fluorescence data signal and calculating a phase correction for the fluorescence detector based on the relative phase between the brightfield data signal and the fluorescence data signal. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate a phase correction for a fluorescence detector based on a brightfield data signal and a fluorescence data signal from the fluorescence detector are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US20210140870A1
公开(公告)日：2021-05-13
申请号：US17153527
申请日：2021-01-20
发明人： Eric D. Diebold , Keegan Owsley , Jonathan Lin
摘要： In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US10823658B2
公开(公告)日：2020-11-03
申请号：US15992097
申请日：2018-05-29
发明人： Eric D. Diebold , Keegan Owsley , Matthew Bahr
摘要： Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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