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    Characterization and Sorting for Particle Analyzers
    1.
    发明申请
    Characterization and Sorting for Particle Analyzers 有权

    公开(公告)号：US20250155352A1

    公开(公告)日：2025-05-15

    申请号：US19020951

    申请日：2025-01-14

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Joseph T. Trotter ,  Keegan O. Owsley

    IPC: G01N15/1429 ,  G01N15/01 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1434 ,  G06F16/20 ,  G06F16/28 ,  G06N3/08 ,  G06N7/06 ,  G06N20/00

    Abstract: Non-parametric transforms such as t-distributed stochastic neighbor embedding (tSNE) are used to analyze multi-parametric data such as data derived from flow cytometry or other particle analysis systems and methods. These transforms may be included for dimensionality reduction and identification of subpopulations (e.g., gating). By nature, non-parametric transforms cannot transform new observations without training a new transformation based on the entire dataset including the new observations. The features described parameterize non-parametric transforms using a neural network thereby allowing a small training dataset to be transformed using non-parametric techniques. The training dataset may then be used to generate an accurate parametric model for assessing additional events in a manner consistent with the initial events.

    Phase-correction of radiofrequency-multiplexed signals
    4.
    发明授权
    Phase-correction of radiofrequency-multiplexed signals 有权

    公开(公告)号：US11808690B2

    公开(公告)日：2023-11-07

    申请号：US18095908

    申请日：2023-01-11

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC: G01N15/14

    CPC classification number: G01N15/1459 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/1434 ,  G01N2015/1402 ,  G01N2015/142 ,  G01N2015/149 ,  G01N2015/1486

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    Phase-Correction Of Radiofrequency-Multiplexed Signals
    5.
    发明公开
    Phase-Correction Of Radiofrequency-Multiplexed Signals 审中-公开

    公开(公告)号：US20230144214A1

    公开(公告)日：2023-05-11

    申请号：US18095908

    申请日：2023-01-11

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC: G01N15/14

    CPC classification number: G01N15/1459 ,  G01N15/1434 ,  G01N15/1425 ,  G01N15/1429 ,  G01N2015/1486 ,  G01N2015/1402 ,  G01N2015/142 ,  G01N2015/149

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    Multi-Modal Fluorescence Imaging Flow Cytometry System
    6.
    发明申请
    Multi-Modal Fluorescence Imaging Flow Cytometry System 有权

    公开(公告)号：US20210140870A1

    公开(公告)日：2021-05-13

    申请号：US17153527

    申请日：2021-01-20

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

    SYSTEMS FOR CELL SORTING BASED ON FREQUENCY-ENCODED IMAGES AND METHODS OF USE THEREOF
    7.
    发明申请
    SYSTEMS FOR CELL SORTING BASED ON FREQUENCY-ENCODED IMAGES AND METHODS OF USE THEREOF 审中-公开

    公开(公告)号：US20200309671A1

    公开(公告)日：2020-10-01

    申请号：US16817080

    申请日：2020-03-12

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Matthew Bahr ,  Keegan Owsley

    IPC: G01N15/14 ,  G06T7/66 ,  G06T7/70 ,  G06T7/136 ,  G06T7/11

    Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.

    Cell Sorting Using A High Throughput Fluorescence Flow Cytometer
    8.
    发明申请
    Cell Sorting Using A High Throughput Fluorescence Flow Cytometer 审中-公开

    公开(公告)号：US20190234863A1

    公开(公告)日：2019-08-01

    申请号：US16382056

    申请日：2019-04-11

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64

    CPC classification number: G01N15/1434 ,  G01N15/1429 ,  G01N15/147 ,  G01N21/6402 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1447 ,  G01N2015/149 ,  G01N2021/6439

    Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

    Phase-correction of radiofrequency-multiplexed signals
    9.
    发明授权
    Phase-correction of radiofrequency-multiplexed signals 有权

    公开(公告)号：US12105010B2

    公开(公告)日：2024-10-01

    申请号：US18373487

    申请日：2023-09-27

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC: G01N15/14 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/149 ,  G01N15/1404

    CPC classification number: G01N15/1459 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/1434 ,  G01N2015/1402 ,  G01N2015/142 ,  G01N2015/1486 ,  G01N15/149

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

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