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    CELL SORTING USING A HIGH THROUGHPUT FLUORESCENCE FLOW CYTOMETER
    6.
    发明申请
    CELL SORTING USING A HIGH THROUGHPUT FLUORESCENCE FLOW CYTOMETER 审中-公开

    公开(公告)号：US20170268981A1

    公开(公告)日：2017-09-21

    申请号：US15462124

    申请日：2017-03-17

    申请人： BD Biosciences

    发明人： Eric Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC分类号： G01N15/14 ,  G01N21/64

    CPC分类号： G01N15/1434 ,  G01N15/1429 ,  G01N15/147 ,  G01N21/6402 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1447 ,  G01N2015/149 ,  G01N2021/6439

    摘要： In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

    DIAMOND MAGNETOMETER
    7.
    发明申请
    DIAMOND MAGNETOMETER 有权

    公开(公告)号：US20170146615A1

    公开(公告)日：2017-05-25

    申请号：US14946912

    申请日：2015-11-20

    申请人： Thomas Wolf ,  Philipp Neumann ,  Jörg Wrachtrup

    发明人： Thomas Wolf ,  Philipp Neumann ,  Jörg Wrachtrup

    IPC分类号： G01R33/032 ,  G01N21/64

    CPC分类号： G01R33/032 ,  G01N21/6402 ,  G01N21/6408 ,  G01N21/6489

    摘要： A magnetometer comprising: a sensor formed of diamond material and comprising a plurality of spin centres; a microwave source configured to subject the plurality of spin centres to microwave pulses; a light source configured to subject the plurality of spin centres to light pulses; and a detector configured to detect a fluorescent output signal emitted from the plurality of spin centres, wherein the magnetometer is configured to integrate the fluorescent output signal over a signal averaging time and process the fluorescent output signal such that a standard deviation of the fluorescent output signal decreases with the square root of the signal averaging time over a time period which spans at least two orders of magnitude in the signal averaging time to achieve a standard deviation of less than 100 picotesla.

    LASER MICROSCOPE AND MICROSCOPY METHOD
    8.
    发明申请
    LASER MICROSCOPE AND MICROSCOPY METHOD 审中-公开
    激光显微镜和显微镜方法

    公开(公告)号：US20170059483A1

    公开(公告)日：2017-03-02

    申请号：US15207394

    申请日：2016-07-11

    申请人： OLYMPUS CORPORATION

    发明人： Ryusuke TANAKA

    IPC分类号： G01N21/64 ,  G02F1/11 ,  G02B21/16 ,  G02B21/06

    CPC分类号： G01N21/6402 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/06 ,  G02B21/16 ,  G02F1/11

    摘要： Fluorescence generated by multiphoton excitation can be observed simultaneously using multiple beams, and multiple points can be observed simultaneously with a high signal-to-noise ratio with low invasiveness. Provided is a laser microscope including: modulation units that apply different modulations to a plurality of ultrashort-pulse laser light beams of the same type emitted from a light source unit; an illumination optical system that simultaneously focuses the plurality of ultrashort-pulse laser light beams, to which the different modulations are applied by the modulation units, onto different positions of a sample; a fluorescence detecting device that detects fluorescence generated at a focal position of each ultrashort-pulse laser light beam and performs photoelectric conversion of the fluorescence; and a demodulation unit that demodulates an output from the fluorescence detecting device based on modulation information from the modulation units.

    摘要翻译： 可以使用多个光束同时观察多光子激发产生的荧光，并且可以同时观察到多个点，具有较低的侵入性的高信噪比。 本发明提供一种激光显微镜，包括：对从光源单元发射的相同类型的多个超短脉冲激光束进行不同调制的调制单元; 照明光学系统，其将通过调制单元施加了不同调制的多个超短脉冲激光束聚焦到样本的不同位置; 荧光检测装置，其检测在每个超短脉冲激光束的焦点处产生的荧光，并进行荧光的光电转换; 以及解调单元，其基于来自调制单元的调制信息来解调来自荧光检测装置的输出。

    SYSTEM AND METHOD FOR ISOTOPIC ANALYSIS OF CALCIUM USING LASER INDUCED FLUORESCENCE
    9.
    发明申请
    SYSTEM AND METHOD FOR ISOTOPIC ANALYSIS OF CALCIUM USING LASER INDUCED FLUORESCENCE 审中-公开
    使用激光诱导荧光的钙同位素分析的系统和方法

    公开(公告)号：US20170052116A1

    公开(公告)日：2017-02-23

    申请号：US15118981

    申请日：2015-02-18

    申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

    发明人： Timothy Steimle

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6402 ,  G01N21/645 ,  G01N21/6486 ,  G01N2201/06113 ,  G01N2201/067

    摘要： Laser-induced fluorescence based optical system and method configured to precisely quantify the relative abundances of calcium (Ca) isotopes in a sample. Optionally, a diode laser is used as a laser source, with its output frequency shifted by two electro-optical modulators to optically excite fluorescence in the calcium-containing sample. The amounts of fluorescence emitted by the various isotopes are measured and compared.

    摘要翻译： 激光诱导的基于荧光的光学系统和方法被配置为精确量化样品中钙（Ca）同位素的相对丰度。 可选地，使用二极管激光器作为激光源，其输出频率由两个电光调制器偏移以光学激发含钙样品中的荧光。 测量和比较各种同位素发射的荧光量。