公开(公告)号：US20190004302A1

公开(公告)日：2019-01-03

申请号：US16102840

申请日：2018-08-14

Applicant: OLYMPUS CORPORATION

Inventor： Kentaro IMOTO ,  Ryusuke TANAKA ,  Hidenori TSUBOI

IPC: G02B21/00 ,  G01N21/64

Abstract: Provided is an image-acquisition apparatus including: a scanning portion that scans illumination light emitted from a light source; an optical system that focuses the scanned illumination light on a sample, while collecting signal light beams generated at the individual scanning positions on the sample; a detector that detects the collected signal light beams and that generates detection signals thereof; a signal controller that is configured to generate periodic signals that are repeated at a predetermined period; a light controller that is configured to temporally control a position or an intensity of the illumination light in accordance with the generated periodic signals; and a computer that is configured to process, in accordance with the generated periodic signals, the detection signals generated, wherein the computer is provided with a frequency shifter for shifting frequencies of the detection signals, and an integrator that integrates the shifted detection signals at an integration time.

公开(公告)号：US20190137746A1

公开(公告)日：2019-05-09

申请号：US16177119

申请日：2018-10-31

Applicant: OLYMPUS CORPORATION

Inventor： Hiroyuki YABUGAKI ,  Ryusuke TANAKA

IPC: G02B21/00 ,  H03L7/091 ,  H03M1/12

Abstract: Provided is a light detecting apparatus including: a phase locked loop portion that generates a sampling clock based on a synchronization signal output from a light source that emits pulsed laser light; an A/D convertor that performs sampling of signal light output from a sample as a result of radiating the laser light thereon in accordance with the sampling clock; and a received-data processing portion that accommodates, every time N items of the sampling data are continuously acquired, the N items of data in a single data sequence. The phase locked loop portion is provided with a clock generating portion that generates a clock that has a frequency that is N times a pulse frequency of the laser light and that is synchronized with a phase of the laser light, and a delay adjusting portion that generates the sampling clock by adjusting a delay amount of the generated clock.

公开(公告)号：US20170059483A1

公开(公告)日：2017-03-02

申请号：US15207394

申请日：2016-07-11

Applicant: OLYMPUS CORPORATION

Inventor： Ryusuke TANAKA

IPC: G01N21/64 ,  G02F1/11 ,  G02B21/16 ,  G02B21/06

CPC classification number: G01N21/6402 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/06 ,  G02B21/16 ,  G02F1/11

Abstract: Fluorescence generated by multiphoton excitation can be observed simultaneously using multiple beams, and multiple points can be observed simultaneously with a high signal-to-noise ratio with low invasiveness. Provided is a laser microscope including: modulation units that apply different modulations to a plurality of ultrashort-pulse laser light beams of the same type emitted from a light source unit; an illumination optical system that simultaneously focuses the plurality of ultrashort-pulse laser light beams, to which the different modulations are applied by the modulation units, onto different positions of a sample; a fluorescence detecting device that detects fluorescence generated at a focal position of each ultrashort-pulse laser light beam and performs photoelectric conversion of the fluorescence; and a demodulation unit that demodulates an output from the fluorescence detecting device based on modulation information from the modulation units.

Abstract translation: 可以使用多个光束同时观察多光子激发产生的荧光，并且可以同时观察到多个点，具有较低的侵入性的高信噪比。 本发明提供一种激光显微镜，包括：对从光源单元发射的相同类型的多个超短脉冲激光束进行不同调制的调制单元; 照明光学系统，其将通过调制单元施加了不同调制的多个超短脉冲激光束聚焦到样本的不同位置; 荧光检测装置，其检测在每个超短脉冲激光束的焦点处产生的荧光，并进行荧光的光电转换; 以及解调单元，其基于来自调制单元的调制信息来解调来自荧光检测装置的输出。