公开(公告)号：US20240361584A1

公开(公告)日：2024-10-31

申请号：US18645424

申请日：2024-04-25

申请人： LEICA MICROSYSTEMS CMS GMBH ,  BioAxial SAS

发明人： Gabriel SIRAT ,  Tom SPERBER ,  Lionel MOISAN ,  Marcus DYBA

IPC分类号： G02B21/06 ,  G01N15/1434 ,  G02B21/16 ,  G02B21/36

CPC分类号： G02B21/06 ,  G01N15/1434 ,  G02B21/16 ,  G02B21/365

摘要： A single-particle localization microscope includes a light source configured to generate illumination light for illuminating a sample region, and an optical illumination system configured to shape the illumination light into a localizing light distribution having a substantially zero intensity minimum at a target point within the sample region. The localizing light distribution is adapted to cause a single particle in a fluorescent state located in the sample region outside the intensity minimum to emit fluorescent light. The optical illumination system is further configured to shape the illumination light into an auxiliary light distribution having a non-zero intensity at the target point such that the auxiliary light distribution is defined in a spatial extent and/or in its shape by the localizing light distribution.

公开(公告)号：US12117600B2

公开(公告)日：2024-10-15

申请号：US17442238

申请日：2020-03-26

申请人： HAMAMATSU PHOTONICS K.K.

发明人： Jiro Yamashita ,  Yasuyuki Tanabe ,  Shunsuke Matsuda ,  Hirotoshi Terada

IPC分类号： G02B21/16 ,  G01N21/64 ,  G02B21/00 ,  G02B26/08 ,  G02B27/14

CPC分类号： G02B21/0048 ,  G01N21/6458 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16 ,  G02B26/0833 ,  G02B27/14

摘要： A confocal microscope unit according to an embodiment includes: a first subunit which includes a light source, a pinhole plate, and a photodetector; a second subunit which includes a light source, a pinhole plate, and a photodetector; a scan mirror which scans excitation light on a sample and guides fluorescence generated from the sample to the first and second subunits; a scan lens which guides the excitation light and guides the fluorescence to the scan mirror; and a main housing which is attachable to a connection port and to which the scan mirror, the scan lens, and the subunits are fixed, wherein the first subunit includes a dichroic mirror that separates the excitation light and fluorescence handled by the own unit from those handled by the second subunit.

公开(公告)号：US20240310617A1

公开(公告)日：2024-09-19

申请号：US18575708

申请日：2022-06-30

申请人： CytoVeris Inc.

发明人： David Fournier ,  Alan Kersey

IPC分类号： G02B21/16 ,  G01N21/64 ,  G02B21/06 ,  G02B21/36

CPC分类号： G02B21/16 ,  G01N21/6458 ,  G01N21/6486 ,  G02B21/06 ,  G02B21/361

摘要： A system and method for imaging a tissue sample is provided. The system includes a dome, at least one excitation light source, and at least one light detector. The dome is configured to surround at least a portion of a tissue sample. The dome has interior surfaces that define a dome interior cavity. The excitation light source is configured to produce light at one or more wavelengths. The excitation light source is in photometric communication with the dome. The dome interior surfaces are configured to reflect the light at the one or more predetermined wavelengths. The dome is configured to cause the light at the one or more predetermined wavelengths to be incident to the exposed surface of the tissue sample in a substantially uniform manner. The light detector is in photometric communication with the dome and configured to detect light emitted or reflected from the tissue sample.

公开(公告)号：US11933728B2

公开(公告)日：2024-03-19

申请号：US18154405

申请日：2023-01-13

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Gary Skinner ,  Geraint Evans ,  Stanley Hong

IPC分类号： G06V10/10 ,  B01L3/00 ,  G01N21/05 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G02B21/36 ,  G02B27/42

CPC分类号： G01N21/6452 ,  B01L3/5085 ,  G01N21/05 ,  G02B21/06 ,  G02B21/16 ,  G02B21/365 ,  G02B27/425 ,  G06V10/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0893 ,  B01L2300/0896

摘要： Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

公开(公告)号：US20240060898A1

公开(公告)日：2024-02-22

申请号：US18452060

申请日：2023-08-18

申请人： Carl Zeiss Microscopy GmbH

发明人： Stanislav Kalinin ,  Dieter Huhse

IPC分类号： G01N21/64 ,  G02B21/16

CPC分类号： G01N21/6486 ,  G02B21/16 ,  G01N2201/121

摘要： A method can be used for correcting background signals in captured measurement values of analog detectors, wherein measurement values of an object captured over a reference time period are analyzed and characteristic values of captured background signals are determined. What is characteristic of this is that a threshold value is determined on the basis of at least one characteristic value and by applying a calculation specification; the threshold value is applied to captured measurement values of an analog detector, and only those measurement values which are greater than the threshold value are used for a subsequent signal evaluation. A microscope for carrying out the method according to the invention is also provided.

公开(公告)号：US11906431B2

公开(公告)日：2024-02-20

申请号：US17178957

申请日：2021-02-18

申请人： NIKON CORPORATION

发明人： Ichiro Sase ,  Yasutoshi Kaneko ,  Tatsuo Fukui

IPC分类号： G02B21/36 ,  G02B21/16 ,  G01N21/64 ,  H04N23/00 ,  G02B21/00

CPC分类号： G01N21/6458 ,  G01N21/64 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/365 ,  G02B21/367 ,  H04N23/00 ,  G01N2201/06113

摘要： A microscope apparatus including: an illumination optical system that radiates activation light to activate some of fluorescent materials included in a sample and excitation light to excite at least some of the activated fluorescent materials; an image forming optical system that: has an objective lens and an astigmatic optical system that generates astigmatism to at least part of fluorescence from the fluorescent materials; and forms an image of the fluorescence; an image-capturing unit that captures an image formed by the image forming optical system; a drive unit that moves an image-capturing position in the sample along an optical axis-direction of the objective lens; and a control unit, wherein the control unit causes the image-capturing unit to capture images in a plurality of numbers of frames respectively at a first image-capturing position and at a second image-capturing position different from the first image-capturing position.

公开(公告)号：US20240044805A1

公开(公告)日：2024-02-08

申请号：US18381379

申请日：2023-10-18

申请人： SamanTree Medical SA

发明人： Bastien Rachet ,  Davor Kosanic ,  Etienne Shaffer

IPC分类号： G01N21/77 ,  G02B21/00 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36 ,  G01N21/64 ,  G01N33/483 ,  G02B21/16

CPC分类号： G01N21/77 ,  G02B21/0032 ,  G02B21/004 ,  G02B21/0076 ,  G02B21/0088 ,  G02B21/26 ,  G02B21/34 ,  G02B21/361 ,  G01N21/6458 ,  G01N21/6428 ,  G01N33/4833 ,  G02B21/16 ,  G02B21/0044 ,  G02B21/24

摘要： The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.

公开(公告)号：US20240019679A1

公开(公告)日：2024-01-18

申请号：US18038930

申请日：2021-11-25

申请人： PreciPoint GmbH

发明人： Fritz Muller

IPC分类号： G02B21/36 ,  G02B21/16

CPC分类号： G02B21/361 ,  G02B21/16 ,  G02B21/362

摘要： A digital microscope includes: a stage for holding a sample; a monochrome digital camera; an optical system, arranged between the stage and the monochrome digital camera; an illumination assembly for illuminating the sample; and a control unit for controlling the monochrome digital camera and the illumination assembly; wherein the illumination assembly is configured to provide illumination with three different wavelength ranges, and wherein the control unit is configured to control the illumination assembly to sequentially provide illumination with the three wavelength ranges and wherein the control unit is configured to control the monochrome digital camera to take separate images during illumination with the three wavelength ranges.

公开(公告)号：US20240019371A1

公开(公告)日：2024-01-18

申请号：US18477975

申请日：2023-09-29

申请人： Micrographia Bio Limited

发明人： Christopher C. THOMPSON

IPC分类号： G01N21/64 ,  G01N1/28 ,  G02B21/36 ,  G02B21/16 ,  G02B21/24

CPC分类号： G01N21/6428 ,  G01N21/6458 ,  G01N1/28 ,  G02B21/367 ,  G02B21/16 ,  G02B21/24

摘要： An imaging system and a method of operating an imaging system for imaging a biological target is provided. The imaging system comprises a fluorescence microscope, a light source, an imaging device, a processor, a probe reagent, and a chemical buffer. The method comprises: acquiring, by the imaging device and through the fluorescence microscope, a first image of the biological target at a first time; determining, by the processor, a first value of an image acquisition metric of the first image; determining, by the processor, a first difference between the first value of the image acquisition metric and a predetermined value of the image acquisition metric; adjusting, by the processor, one or more physical parameters of the imaging system based on the first difference, wherein the one or more physical parameters of the imaging system comprise one or more of: an illumination characteristic of the light source, concentration of the probe reagent, and concentration of the chemical buffer; and repeating the steps for a second image acquired at a second time subsequent to the first time.

公开(公告)号：US11874453B2

公开(公告)日：2024-01-16

申请号：US17311179

申请日：2019-09-29

申请人： GENEMIND BIOSCIENCES COMPANY LIMITED

发明人： Jifang Qi ,  Zhiliang Zhou ,  Zefei Jiang ,  Qin Yan

IPC分类号： G02B21/36 ,  G02B27/10 ,  G01N21/64 ,  G02B21/00 ,  G02B21/02 ,  G02B21/06 ,  G02B21/16 ,  G02B27/30

CPC分类号： G02B21/361 ,  G01N21/6458 ,  G02B21/0004 ,  G02B21/02 ,  G02B21/06 ,  G02B21/16 ,  G02B27/10 ,  G02B27/30 ,  G01N2021/6478

摘要： An optical system including a first light source, a first lens, and a light splitting module, wherein the light splitting module has a first splitter, a second lens, a first camera and a second camera, the first lens is configured for receiving a first light beam from the first light source and collimating the first light beam onto a sample, and for receiving and collimating a light beam from the sample, the second lens is configured for focusing the collimated light beam from the first lens to the first camera and the second camera, the first splitter is configured for splitting the focused light beam from the second lens into a second light beam and a third light beam, the first camera is configured for receiving the second light beam, and the second camera is configured for receiving the third light beam.