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公开(公告)号：US11421272B2
公开(公告)日：2022-08-23
申请号：US17417035
申请日：2019-09-29
发明人： Ruitao Sun , Songzhen Zhang , Zefei Jiang , Zhiliang Zhou , Qin Yan
摘要： Disclosed are a positioning method, a positioning apparatus and a sequencing system (300). The sequencing system (300) comprises an imaging assembly (110) and a mobile platform (102), wherein the imaging assembly (110) comprises an optical axis (OP), the mobile platform (102) bears a reaction apparatus (200), the stroke of the mobile platform (102) in a first direction is controlled by means of a limiting switch, and the stroke comprises a first limit. The method comprises the steps: (a) moving, by a first step distance, the mobile platform (102) in a direction approaching the first limit, until the distance between a current position of the reaction apparatus (200) and the previous position of the reaction apparatus (200) is less than the first step distance; (b) moving the mobile platform (102) in a direction that is the opposite of the moving direction in step (a); (c) moving the mobile platform (102) in the moving direction in step (a), until the mobile platform (102) reaches the first limit; and (d) moving, by a preset distance, the mobile platform (102) in the direction that is the opposite of the moving direction in step (a), so that the positions of the optical axis (OP) and the reaction apparatus (200) satisfy a preset relationship. According to the method, the quick and precise positioning of the mobile platform (102) can be realized by means of moving back and forth in the first direction, such that the positions of the optical axis (OP) and the reaction apparatus (200) satisfy a preset relationship.
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公开(公告)号：US10427154B2
公开(公告)日：2019-10-01
申请号：US15752412
申请日：2016-08-12
IPC分类号： B01L3/00 , C12M1/00 , C12M1/34 , C12Q1/68 , C12Q1/6837
摘要： Disclosed is a chip. The chip comprises a substrate (1) and a base layer (2) in pressing arrangement with the substrate; the substrate comprises a first surface (1a) and a second surface (1b) in opposite arrangement, reaction tank arrays formed by a plurality of flowing channels (11) are arranged on the first surface of the substrate at intervals, two oppositely arranged side walls (111, 112) of each flowing channel (11) stretch along the length direction of the flowing channel (11) and intersect at two ends of the flowing channel to form two tapered tail ends (113) with included angles, and a fluid inlet hole (12) and a fluid outlet hole (13) which are communicated with the second surface of the substrate are respectively provided on the surfaces of the two tapered tail ends (113); and the base layer (2) comprises a transparent base (21) and a spacing layer (22) arranged on the surface of the transparent base, the spacing layer (22) is in contact with the firs surface (1a) of the substrate, and a corrosion groove is provided on the spacing layer (22) corresponding to a position where the flowing channel (11) is located. The flow field distribution of the chip is good, the deformation rate of a base in the chip is low, and the fluid in the chip can be fully flushed or replaced. Also disclosed is an application of the chip.
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公开(公告)号：US11575823B2
公开(公告)日：2023-02-07
申请号：US17746838
申请日：2022-05-17
发明人： Linsen Li , Ruitao Sun , Jiahong Xu , Zhiliang Zhou , Zefei Jiang , Qin Yan
摘要： The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.
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公开(公告)号：US11482304B2
公开(公告)日：2022-10-25
申请号：US16635996
申请日：2017-08-02
发明人： Weibin Xu , Huan Jin , Qin Yan , Zefei Jiang , Zhiliang Zhou
摘要： The disclosure discloses an alignment method, device, and system. The alignment method includes: converting each read into a set of short fragments corresponding to the read to obtain a plurality of sets of short fragments; determining a corresponding position of the short fragment in a reference library to obtain a first positioning result, wherein the reference library is a hash table constructed based on a reference sequence, the reference library includes a plurality of entries, one entry of the reference library corresponds to one seed sequence, and the seed sequence is capable of matching at least one sequence on the reference sequence, a distance between two seed sequences corresponding to two adjacent entries of the reference library on the reference sequence is less than a length of the short fragment; removing a short fragment positioned on any one of the adjacent entries of the reference library in the first positioning result to obtain a second positioning result; and extending based on short fragments from the same read in the second positioning result to obtain an alignment result of the read. The alignment method can efficiently and accurately process and position sequencing data.
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公开(公告)号：US11384390B2
公开(公告)日：2022-07-12
申请号：US15855215
申请日：2017-12-27
发明人： Jianfeng Xu , Ruitao Sun , Zefei Jiang , Zhiliang Zhou , Qin Yan
IPC分类号： C12Q1/6869 , G01N35/00 , G02B7/28 , B01L3/00 , G01N21/64 , H04N5/235 , G01N35/10 , H04N5/232
摘要： The present disclosure relates to base sequence determination. A base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit is defined as: a second biochemical reaction—a first biochemical reaction—photographing. A method includes, after initiation steps, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: using the fluid device to perform the first biochemical reaction of the sample on the first component, using the optical device to photograph the sample on the first component after the first biochemical reaction, and using the fluid device to perform the first biochemical reaction of the sample on the second component.
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公开(公告)号：US11368614B2
公开(公告)日：2022-06-21
申请号：US17262663
申请日：2019-07-23
发明人： Linsen Li , Ruitao Sun , Jiahong Xu , Zhiliang Zhou , Zefei Jiang , Qin Yan
摘要： The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.
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公开(公告)号：US11874453B2
公开(公告)日：2024-01-16
申请号：US17311179
申请日：2019-09-29
发明人： Jifang Qi , Zhiliang Zhou , Zefei Jiang , Qin Yan
IPC分类号： G02B21/36 , G02B27/10 , G01N21/64 , G02B21/00 , G02B21/02 , G02B21/06 , G02B21/16 , G02B27/30
CPC分类号： G02B21/361 , G01N21/6458 , G02B21/0004 , G02B21/02 , G02B21/06 , G02B21/16 , G02B27/10 , G02B27/30 , G01N2021/6478
摘要： An optical system including a first light source, a first lens, and a light splitting module, wherein the light splitting module has a first splitter, a second lens, a first camera and a second camera, the first lens is configured for receiving a first light beam from the first light source and collimating the first light beam onto a sample, and for receiving and collimating a light beam from the sample, the second lens is configured for focusing the collimated light beam from the first lens to the first camera and the second camera, the first splitter is configured for splitting the focused light beam from the second lens into a second light beam and a third light beam, the first camera is configured for receiving the second light beam, and the second camera is configured for receiving the third light beam.
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公开(公告)号：US20220298567A1
公开(公告)日：2022-09-22
申请号：US17838104
申请日：2022-06-10
发明人： Jianfeng Xu , Ruitao Sun , Zefei Jiang , Zhiliang Zhou , Qin Yan
摘要： The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.
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9.发明授权公开(公告)号：US11170506B2
公开(公告)日：2021-11-09
申请号：US17270418
申请日：2018-08-22
发明人： Linsen Li , Weibin Xu , Huan Jin , Zefei Jiang , Zhiliang Zhou , Qin Yan
摘要： A method for constructing a sequencing template based on an image, a device, and a system. The image includes first, second, third and fourth images of one same field of view corresponding to base extensions of A, T/U, G, and C respectively; the first, second, third and fourth images respectively include images M1 and M2, images N1 and N2, images P1 and P2, and images Q1 and Q2; the method includes combining any two of the images M1, M2, N1, N2, P1, P2, Q1, and Q2to perform bright spot matching, and enabling such images to participate in the combination for at least one time to obtain a plurality of combined images including first coincident bright spots, and merging the first coincident bright spots on the plurality of combined images to obtain a bright spot set corresponding to the sequencing template.
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公开(公告)号：US10981164B2
公开(公告)日：2021-04-20
申请号：US16554372
申请日：2019-08-28
IPC分类号： B01L3/00 , C12M1/00 , C12M1/34 , C12Q1/68 , C12Q1/6837
摘要： Disclosed is a chip. The chip comprises a substrate (1) and a base layer (2) in pressing arrangement with the substrate; the substrate comprises a first surface (1a) and a second surface (1b) in opposite arrangement, reaction tank arrays formed by a plurality of flowing channels (11) are arranged on the first surface of the substrate at intervals, two oppositely arranged side walls (111, 112) of each flowing channel (11) stretch along the length direction of the flowing channel (11) and intersect at two ends of the flowing channel to form two tapered tail ends (113) with included angles, and a fluid inlet hole (12) and a fluid outlet hole (13) which are communicated with the second surface of the substrate are respectively provided on the surfaces of the two tapered tail ends (113); and the base layer (2) comprises a transparent base (21) and a spacing layer (22) arranged on the surface of the transparent base, the spacing layer (22) is in contact with the firs surface (1a) of the substrate, and a corrosion groove is provided on the spacing layer (22) corresponding to a position where the flowing channel (11) is located. The flow field distribution of the chip is good, the deformation rate of a base in the chip is low, and the fluid in the chip can be fully flushed or replaced. Also disclosed is an application of the chip.
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