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公开(公告)号：US20210131945A1
公开(公告)日：2021-05-06
申请号：US17147063
申请日：2021-01-12
申请人： BD BIOSCIENCES
发明人： Eric Diebold , Keegan Owsley , Jonathan Lin , Matthew Bahr
摘要： In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.
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公开(公告)号：US10324019B2
公开(公告)日：2019-06-18
申请号：US15462124
申请日：2017-03-17
申请人： BD Biosciences
发明人： Eric Diebold , Keegan Owsley , Jonathan Lin
摘要： In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号：US10935485B2
公开(公告)日：2021-03-02
申请号：US15593959
申请日：2017-05-12
申请人： BD Biosciences
发明人： Eric Diebold , Keegan Owsley , Jonathan Lin , Matthew Bahr
摘要： In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.
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公开(公告)号：US20170328826A1
公开(公告)日：2017-11-16
申请号：US15593959
申请日：2017-05-12
申请人： BD Biosciences
发明人： Eric Diebold , Keegan Owsley , Jonathan Lin , Matthew Bahr
CPC分类号： G01N15/1475 , G01N15/1429 , G01N15/147 , G01N21/6456 , G01N2015/0065 , G01N2015/1006 , G01N2015/144 , G01N2201/06113 , G01N2201/10 , G01N2201/12 , G01N2201/129 , G06T7/0012 , G06T7/246 , G06T7/521 , G06T2207/30024
摘要： In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.
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公开(公告)号：US20170268981A1
公开(公告)日：2017-09-21
申请号：US15462124
申请日：2017-03-17
申请人： BD Biosciences
发明人： Eric Diebold , Keegan Owsley , Jonathan Lin
CPC分类号： G01N15/1434 , G01N15/1429 , G01N15/147 , G01N21/6402 , G01N21/6428 , G01N2015/1006 , G01N2015/1447 , G01N2015/149 , G01N2021/6439
摘要： In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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