Abstract:
A fluorescence observation apparatus includes a fluorescence observation unit, which includes a scanner that scans ultrashort pulsed laser light from a light source, a pupil projection lens that focuses the laser light scanned by the scanner, an image-forming lens that converts the focused laser light to substantially collimated light and causes the laser light to be incident on an objective lens, and a dichroic mirror that splits off fluorescence that is generated by the laser light focused on a sample by the objective lens and is collected by the objective lens. A photodetector detects the fluorescence split off by the dichroic mirror. A multi-mode optical fiber connects the fluorescence observation unit and the photodetector. A swiveling mechanism causes the fluorescence observation unit to swivel about an axis near the focal position of the objective lens. And a light-source optical fiber connects the light source and the fluorescence observation unit.
Abstract:
A fluorescence observation unit includes a scanner that scans ultrashort pulsed laser light, a pupil projection lens that focuses the scanned ultrashort pulsed laser light, an image-forming lens that converts the focused ultrashort pulsed laser light to substantially collimated light and causes the ultrashort pulsed laser light to be incident on the objective lens, and a dichroic mirror that splits off, from the optic path of the ultrashort pulsed laser light, fluorescence generated in a sample due to irradiation with the ultrashort pulsed laser light and collected by the objective lens. The image-forming lens includes a first optical system having positive refractive power, and a second optical system having negative refractive power and disposed at a position closer to the scanner than the first optical system is. The dichroic mirror is disposed between the first optical system and the second optical system.
Abstract:
With the object of preventing deterioration of or damage to a photodetector caused by excessive light by more reliably preventing the excessive light from entering the photodetector, a microscope of the present invention is provided with a high-sensitivity detector, such as an HPD, a GaAsP, an EM-CCD or the like, that detects observation light coming from a specimen, a box-shaped casing that has an opening that allows contained items to be placed therein and removed therefrom and that covers the high-sensitivity detector, a door that can close off the opening of the casing, a switch that restricts light detection by the high-sensitivity detector by turning on and off a drive voltage to be applied to the high-sensitivity detector, and an opening restricting mechanism that allows the opening of the casing in the closed state imposed by the door to be opened only when the light detection by the high-sensitivity detector is restricted by the switch.