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    Phase-Correction Of Radiofrequency-Multiplexed Signals
    1.
    发明公开
    Phase-Correction Of Radiofrequency-Multiplexed Signals 审中-公开

    公开(公告)号：US20240019354A1

    公开(公告)日：2024-01-18

    申请号：US18373487

    申请日：2023-09-27

    申请人： Becton, Dickinson and Company

    发明人： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC分类号： G01N15/14

    CPC分类号： G01N15/1459 ,  G01N15/1434 ,  G01N15/1425 ,  G01N15/1429 ,  G01N2015/1486 ,  G01N2015/1402 ,  G01N2015/142 ,  G01N2015/149

    摘要： Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    IMAGING FLOW CYTOMETER
    3.
    发明公开
    IMAGING FLOW CYTOMETER 审中-公开

    公开(公告)号：US20230175949A1

    公开(公告)日：2023-06-08

    申请号：US17924135

    申请日：2021-09-10

    申请人： The University of Tokyo

    发明人： Sadao OTA ,  Masashi UGAWA

    IPC分类号： G01N15/14

    CPC分类号： G01N15/1434 ,  G01N15/1404 ,  G01N2015/1443 ,  G01N2015/142

    摘要： An imaging flow cytometer includes: a flow channel in which an observation object flows and a length in a width direction is longer than a length in a height direction; an acoustic element configured to apply acoustic waves as standing waves to the flow channel; a light source that irradiates the flow channel with illumination light; an image sensor configured to image at least a line included in a cross section of the observation object crossing a flow line direction which is a direction in which the observation object flows in the flow channel by measuring or imaging the observation object passing through a position irradiated with the illumination light; and circuitry configured to generate an image in which the observation object is scanned in the flow line direction on the basis of a plurality of captured images acquired by the imaging unit imaging the line in a time series.