公开(公告)号：US12111248B2

公开(公告)日：2024-10-08

申请号：US16382489

申请日：2019-04-12

申请人： Labrador Diagnostics LLC

发明人： Karan Mohan ,  Chinmay Pangarkar ,  James R. Wasson

IPC分类号： G01N21/03 ,  B01L3/00 ,  B01L9/00 ,  G01N1/30 ,  G01N15/10 ,  G01N21/05 ,  G01N21/17 ,  G01N21/27 ,  G01N21/64 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/569 ,  G02B7/09 ,  G02B21/00 ,  G02B21/08 ,  G02B21/12 ,  G02B21/16 ,  G02B21/24 ,  G02B21/34 ,  G02B21/36 ,  G01N15/01 ,  G01N15/14

CPC分类号： G01N21/0303 ,  B01L3/502715 ,  B01L3/5085 ,  B01L9/523 ,  G01N1/30 ,  G01N15/1012 ,  G01N21/05 ,  G01N21/17 ,  G01N21/27 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/487 ,  G01N33/49 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/56972 ,  G02B7/09 ,  G02B21/00 ,  G02B21/0076 ,  G02B21/088 ,  G02B21/125 ,  G02B21/16 ,  G02B21/244 ,  G02B21/34 ,  G02B21/365 ,  G02B21/367 ,  B01L2300/0654 ,  B01L2300/168 ,  G01N15/01 ,  G01N2015/1014 ,  G01N2015/1486 ,  G01N2021/1738 ,  G01N2021/6439 ,  G01N2201/061 ,  G01N2201/068 ,  G01N2201/12 ,  G01N2333/70589 ,  G01N2333/70596

摘要： Methods, devices, apparatus, and systems are provided for image analysis. Methods of image analysis may include observation, measurement, and analysis of images of biological and other samples; devices, apparatus, and systems provided herein are useful for observation, measurement, and analysis of images of such samples. The methods, devices, apparatus, and systems disclosed herein provide advantages over other methods, devices, apparatus, and systems.

公开(公告)号：US20240329055A1

公开(公告)日：2024-10-03

申请号：US18681988

申请日：2022-07-31

申请人： University of Rochester

发明人： James Lionel McGrath ,  Jonathan David Flax ,  Kilean Scott Lucas ,  Samuel Norman Walker

IPC分类号： G01N33/68 ,  B01L3/00 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N33/6863 ,  B01L3/502761 ,  G01N33/54386 ,  G01N33/56966 ,  B01L2200/0647 ,  B01L2300/0654

摘要： A device and related methods for the detection of biomarkers is disclosed. The device includes a nanoporous membrane having a plurality of pores. The nanoporous membrane is configured to capture extracellular vesicles or other biological material. An assay is used, to determine the level of biomarkers of interest that are contained with the captured extracellular vesicles or other biological material

公开(公告)号：US20240329036A1

公开(公告)日：2024-10-03

申请号：US18647207

申请日：2024-04-26

申请人： SYSMEX CORPORATION

发明人： Chihiro TAKARA ,  Takanori KOMAKI ,  Masaya YAMAUCHI

IPC分类号： G01N33/53 ,  G01N21/64 ,  G01N33/569

CPC分类号： G01N33/5308 ,  G01N21/6428 ,  G01N33/56966 ,  G01N2021/6439

摘要： Disclosed is a blood cell analysis method comprising: acquiring fluorescence information, first scattered light information, and second scattered light information generated by irradiating a measurement sample comprising a particle stained with a fluorescent dye capable of binding to a nucleic acid with light of a first wavelength and light of a second wavelength; specifying a population of nucleated cells from particles in the measurement sample based on the fluorescence information; and specifying a nucleated red blood cell from the population of nucleated cells based on the first scattered light information and the second scattered light information of the population of nucleated cells.

公开(公告)号：US20240301033A1

公开(公告)日：2024-09-12

申请号：US18436312

申请日：2024-02-08

申请人： Janssen Biotech, Inc.

发明人： Rebecca Hawkins ,  Steven Jacobs ,  Manuel Sepulveda

IPC分类号： C07K14/78 ,  A61K49/00 ,  A61K51/08 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569 ,  G01N33/574 ,  G01N33/68

CPC分类号： C07K14/78 ,  A61K49/0002 ,  A61K51/088 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569 ,  G01N33/56966 ,  G01N33/574 ,  G01N33/57492 ,  G01N33/68 ,  G01N33/6887 ,  G01N2333/70517 ,  G01N2333/78

摘要： Fibronectin type III domains (FN3) that specifically bind to CD8A, related polynucleotides capable of encoding CD8A-specific FN3 domains, cells expressing the FN3 domains, as well as associated vectors, and detectably labeled FN3 domains are useful in therapeutic and diagnostic applications.

公开(公告)号：US12072333B2

公开(公告)日：2024-08-27

申请号：US16346562

申请日：2017-11-02

申请人： UNIVERSITY OF IOWA RESEARCH FOUNDATION ,  SYNDERBIO, INC.

发明人： Michael D. Henry ,  Benjamin Krog ,  Sarah Vigmostad ,  Mike Cable

IPC分类号： G01N33/569 ,  C12Q1/6806 ,  G01N1/28 ,  G01N1/34 ,  G01N33/483

CPC分类号： G01N33/56966 ,  C12Q1/6806 ,  G01N1/286 ,  G01N33/4833 ,  G01N2001/2866 ,  G01N1/34

摘要： In some embodiments, the present disclosure provides an apparatus and methods for selectively lysing cells from a cell suspension including disassociating a population of cells from a tissue sample, the population of cells including a population of one or more viable cells and a population of one or more dead and dying cells, exposing the population of cells to at least one pulse of fluid shear stress having a force along a conduit wall of from about 500 dyn/cm2 to about 2500 dyn/cm2 to substantially lyse the population of one or more dead or dying cells, leaving the population of one or more viable cells substantially intact, and collecting the population of one or more viable cells.

公开(公告)号：US12046331B2

公开(公告)日：2024-07-23

申请号：US15847569

申请日：2017-12-19

申请人： Hematologics, Inc.

发明人： Michael R. Loken ,  Sanjaya N. Joshi

IPC分类号： G16B40/10 ,  G01N15/01 ,  G01N15/14 ,  G01N33/50 ,  G01N33/569 ,  G01N33/574 ,  G06F18/23 ,  G06F18/40 ,  G06V10/762 ,  G06V10/778 ,  G06V20/69 ,  G16B25/00 ,  G16B40/00 ,  G16B45/00

CPC分类号： G16B40/10 ,  G01N15/1456 ,  G01N33/5091 ,  G01N33/56966 ,  G01N33/56972 ,  G01N33/574 ,  G01N33/57492 ,  G06F18/23 ,  G06F18/41 ,  G06V10/762 ,  G06V10/7788 ,  G06V20/698 ,  G16B40/00 ,  G01N15/01 ,  G16B25/00 ,  G16B45/00

摘要： A system, method, and article for diagnosing a test set of biological cells. For example, in one embodiment a normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the set of clusters.

公开(公告)号：US20240241104A1

公开(公告)日：2024-07-18

申请号：US18622012

申请日：2024-03-29

申请人： Axogen Corporation

发明人： Curt DEISTER

IPC分类号： G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01R33/56 ,  G01R33/563

CPC分类号： G01N33/4833 ,  G01N33/5026 ,  G01N33/56966 ,  G01R33/5608 ,  G01R33/56341

摘要： Techniques and systems are disclosed for a bioassay that is an in vitro mimic of peripheral nerve generation using the sensory neurons that innervate the peripheral nervous system. In some embodiments, the techniques may assist in detecting the bioactivity or potency of nerve grafts (e.g., processed, acellular human allografts) for fostering or supporting peripheral nerve regeneration. In various embodiments, techniques comprise affixing a harvested sensory neuron (e.g., a DRG) to a nerve graft segment to form a test construct; culturing the test construct in a medium; analyzing the test construct to indicate the amount of outgrowing peripheral nerve structure; and determining the potency of the nerve graft from a metric derived from the analysis. In some embodiments, techniques and materials may be used to test the effect of a varied test condition on peripheral nerve growth.

公开(公告)号：US20240230646A9

公开(公告)日：2024-07-11

申请号：US18449154

申请日：2023-08-14

申请人： University of Southern California

发明人： Preet M. Chaudhary

IPC分类号： G01N33/569 ,  C07K16/28 ,  G01N33/50 ,  G01N33/58

CPC分类号： G01N33/56972 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2866 ,  C07K16/2878 ,  C07K16/2887 ,  C07K16/2896 ,  G01N33/5023 ,  G01N33/5044 ,  G01N33/56966 ,  G01N33/581 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/43 ,  C07K2319/60 ,  G01N2333/70503 ,  G01N2333/72 ,  G01N2800/52

摘要： Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.

公开(公告)号：US20240229166A9

公开(公告)日：2024-07-11

申请号：US18277612

申请日：2022-02-18

申请人： Massachusetts Institute of Technology ,  The Children's Medical Center Corporation ,  University of Mississippi Medical Center

发明人： Alexander K. Shalek ,  Jose Ordovas-Montanes ,  Carly Ziegler ,  Sarah Glover ,  Bruce Horwitz ,  Vincent Miao ,  Anna Owings ,  Andrew Navia ,  Ying Tang ,  Joshua Bromley

IPC分类号： C12Q1/70 ,  C12Q1/6874 ,  G01N33/569

CPC分类号： C12Q1/701 ,  C12Q1/6874 ,  G01N33/56966 ,  C12Q2600/112 ,  C12Q2600/158

摘要： The subject matter disclosed herein is generally directed to stratifying and treating coronavirus infections based on intrinsic immune states.

公开(公告)号：US20240218032A1

公开(公告)日：2024-07-04

申请号：US18556860

申请日：2022-04-22

申请人： Navigo Proteins GmbH

发明人： Eva Bosse-Doenecke ,  Manja Gloser-Braeunig ,  Mathias Kahl ,  Hanna Bobolowski ,  Jonathan Lotze

IPC分类号： C07K14/47 ,  A61K47/64 ,  A61K49/00 ,  A61K51/08 ,  G01N33/569

CPC分类号： C07K14/4703 ,  A61K47/64 ,  A61K49/0056 ,  A61K49/006 ,  A61K51/08 ,  G01N33/56966

摘要： The present invention relates to new ubiquitin derived molecules that are specific for Programmed Death Ligand 1 (PD-L1). The PD-L1 specific molecules of the invention inhibit the interaction between PD1 and PD-L1. The invention further refers to PD-L1 specific Affilin® proteins that further comprise a diagnostically or therapeutically active component. Further aspects of the invention cover the use of these PD-L1 binding proteins in medicine, for example, in diagnosis and therapy of PD-L1 related cancer.