公开(公告)号：US20240361318A1

公开(公告)日：2024-10-31

申请号：US18647863

申请日：2024-04-26

Applicant: Waters Technologies Corporation

Inventor： Kevin Wyndham ,  Beatrice Muriithi ,  Martin Gilar ,  Yeliz Tunc Sarisozen

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543 ,  G01N33/74

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/74 ,  G01N2333/015 ,  G01N2333/62 ,  G01N2469/10

Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).

公开(公告)号：US20240353407A1

公开(公告)日：2024-10-24

申请号：US18677132

申请日：2024-05-29

Applicant: DENSO CORPORATION

Inventor： Kei HAYAKAWA ,  Mana ASANO ,  Akira NUKAZUKA ,  Kazuhisa NAKAGAWA ,  Mai NIIMOTO ,  Kazuhiko KANO

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/56983 ,  G01N33/5308 ,  G01N33/54386 ,  G01N2333/165 ,  G01N2469/10

Abstract: An analysis method for a target substance includes: mixing a conjugate and a sample including the target substance to produce a mixture, the conjugate including a labeled substance and a first binding substance having activity to bind to the target substance; bringing the mixture into contact with a second binding substance fixed to a base; and detecting a phenomenon caused by the labeled substance bound to the target substance that is bound to the second binding substance. The first binding substance is a substance including a nucleic acid, or a substance including an amino acid. The base is provided in each of regions. The target substance to which the second binding substance binds is different among the regions.

公开(公告)号：US20240353402A1

公开(公告)日：2024-10-24

申请号：US18643170

申请日：2024-04-23

Applicant: TBP Diagnostics, LLC

Inventor： Lawrence Silbart ,  Thanh Nguyen

IPC: G01N33/543 ,  G01N33/53 ,  G01N33/569

CPC classification number: G01N33/54388 ,  G01N33/5308 ,  G01N33/56905 ,  G01N33/56911 ,  G01N33/56983 ,  G01N2333/08 ,  G01N2333/11 ,  G01N2333/183 ,  G01N2333/195 ,  G01N2333/20 ,  G01N2469/10

Abstract: Systems and methods are described herein for a diluent-containing tube comprising a beveled grinding surface and operable to receive a plunger comprising a complementary beveled grinding surface. Solid matrices placed within the diluent can be ground between the grinding surfaces to release molecules into the diluent for detection. The plunger may further comprise a groove containing a test strip operable to be deployed into the diluent after grinding for detection of the released molecules. In certain embodiments, the test strip may be a lateral flow immunoassay rapid antigen detection (LFA) test strip. The solid matrix may be a tick and the molecules may be antigens or pathogens related to tick-borne illnesses.

公开(公告)号：US12117442B2

公开(公告)日：2024-10-15

申请号：US18339347

申请日：2023-06-22

Applicant: Depixus SAS

Inventor： Gordon Hamilton ,  Jimmy Ouellet ,  Zhen Wang ,  Henning Labuhn

IPC: G01N33/53 ,  G01N33/543

CPC classification number: G01N33/54373 ,  G01N33/5308 ,  G01N33/54326 ,  G01N2500/04

Abstract: Disclosed herein are methods of screening candidate binding compounds to a plurality of target nucleic acids either of the same sequence or of different sequences utilizing a device configured to detect changes in the folding and unfolding force of the target nucleic acid upon binding to various candidate binding compounds. In the presence of a plurality of nucleic acid sequences, the methods described herein also can rapidly determine the specificity of the small molecule or polypeptide to a nucleic acid structure. Also, disclosed herein are methods of measuring binding kinetics of binding compounds to a target nucleic acid using a device configured to detect changes in the folding and unfolding force of the target nucleic acid upon binding to varying concentrations of the candidate binding compound.

公开(公告)号：US20240318255A1

公开(公告)日：2024-09-26

申请号：US18260011

申请日：2021-12-29

Applicant: Belgian Volition SRL (BE/BE)

Inventor： Jacob Vincent MICALLEF ,  Mark Edward ECCLESTON ,  Marielle HERZOG

IPC: C12Q1/6886 ,  G01N33/53 ,  G01N33/68

CPC classification number: C12Q1/6886 ,  G01N33/5308 ,  G01N33/6854 ,  G01N33/6875

Abstract: The invention relates to methods for detecting disease in a subject by means of a minimally invasive body fluid test for non-nucleosomal cell free DNA fragments. The invention also relates to the measurement or detection of circulating cell free DNA fragments that include a transcription factor binding site sequence as an indicator of the presence of disease in a subject.

公开(公告)号：US12099056B2

公开(公告)日：2024-09-24

申请号：US17554268

申请日：2021-12-17

Applicant: ORASURE TECHNOLOGIES, INC.

Inventor： Giffin Daughtridge ,  Keith Kardos ,  Sharanappa Basappa Rajur ,  Kim Hwa-Ok ,  Asharani Dhondiram Kadam

IPC: G01N33/53 ,  C07K16/44 ,  G01N33/543 ,  G01N33/577

CPC classification number: G01N33/5308 ,  C07K16/44 ,  G01N33/54388 ,  G01N33/577 ,  C07K2317/565 ,  G01N2430/60

Abstract: The invention provides novel compounds, reagents, systems, and methods for detecting a metabolite related to a NRTI in a biological sample, and uses thereof in monitoring adherence to pre-exposure prophylaxis. Such reagents comprise NRTI derivatives, analogs, NRTI derivatives conjugates, along with antibodies directed to same, which are useful for antibody-based methods, such as a lateral flow immunoassay and other point of care devices.

公开(公告)号：US12098370B2

公开(公告)日：2024-09-24

申请号：US17399934

申请日：2021-08-11

Applicant: SomaLogic Operating Co., Inc.

Inventor： Dominic Zichi ,  Sheri K Wilcox ,  Chris Bock ,  Daniel J. Schneider ,  Bruce Eaton ,  Larry Gold

IPC: C12N15/115 ,  C12N9/74 ,  C12N15/10 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/53 ,  G01N33/58

CPC classification number: C12N15/115 ,  C12N9/6429 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/5308 ,  G01N33/58 ,  C12N2310/16 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/333 ,  C12N2310/334 ,  C12N2310/335 ,  C12N2310/336 ,  C12N2330/31 ,  C12Q1/6811 ,  C12Q2525/205 ,  C12Q1/6816 ,  C12Q2565/514 ,  C12Q2525/205 ,  C12Q2525/161 ,  C12Q1/6832 ,  C12Q2537/101 ,  C12Q2525/205 ,  C12Q2523/313 ,  C12Q1/6834 ,  C12Q2525/205 ,  C12Q2525/161

Abstract: The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

公开(公告)号：US20240310387A1

公开(公告)日：2024-09-19

申请号：US18575507

申请日：2022-06-28

Applicant: BERYSOL GMBH

Inventor： Lutz SCHOMBURG

IPC: G01N33/68 ,  G01N33/53

CPC classification number: G01N33/6893 ,  G01N33/5308 ,  G01N2800/50

Abstract: Instant invention discloses a method for evaluating the risk of selenium (Se) deficiency in a subject by determining both the amount of selenium or selenoprotein P and the amount of antibodies against selenoprotein P, estimating or calculating a risk index score for the Se deficiency of said subject using the obtained values, and the use of the method of the invention or of the risk index score obtained according to the invention in the monitoring of Se supplementation, especially in subjects suffering from a disease related to a selenium deficit.

公开(公告)号：US12092635B2

公开(公告)日：2024-09-17

申请号：US18386066

申请日：2023-11-01

Applicant: 10X GENOMICS, INC.

Inventor： Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Andrew Kohlway ,  Luigi Jhon Alvarado Martinez ,  Katherine Pfeiffer ,  Andrew D. Price

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B30/04 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/532 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B30/04 ,  C40B50/06 ,  C40B70/00

Abstract: The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.

公开(公告)号：US20240302384A1

公开(公告)日：2024-09-12

申请号：US18547759

申请日：2022-03-03

Applicant: APHP (ASSISTANCE PUBLIQUE - HÔPITAUX DE PARIS ) ,  UNIVERSITE PARIS ,  INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE ) ,  IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE

Inventor： Yoram BOUHNIK ,  Alexandre NUZZO ,  Olivier CORCOS ,  Marc-Emmanuel DUMAS ,  Antonis MYRIDAKIS ,  Dominique GAUGUIER

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/92

CPC classification number: G01N33/6893 ,  G01N33/5308 ,  G01N33/92 ,  G01N2570/00 ,  G01N2800/06 ,  G01N2800/7019

Abstract: A metabolomic signature of acute mesenteric ischemia (AMI) and the determination thereof in a method for identifying a subject suffering or being at risk of suffering from AMI. Also, a kit that includes elements for determining the metabolomic signature of AMI and implementing the method for identifying a subject suffering or being at risk of suffering from AMI.