公开(公告)号：US20240353434A1

公开(公告)日：2024-10-24

申请号：US18238544

申请日：2023-08-28

申请人： Institute of Microbiology, Guangdong Academy of Sciences (Guangdong Detection Center of Microbiology

发明人： Meiying XU ,  Jinna ZHANG ,  Ariguo WANG ,  Sihua LAI

IPC分类号： G01N33/94 ,  G01N1/34 ,  G01N33/68

CPC分类号： G01N33/9466 ,  G01N1/34 ,  G01N33/6851

摘要： A method for simultaneous and efficient detection of residues of 36 antidepressants in sludge includes extraction of target drugs, purification of an extraction solution, detection by the technique of ultra-performance liquid chromatography-tandem mass spectrometry, and qualitative and quantitative analysis of the antidepressants. Based on the detection technique of ultra-performance liquid chromatography-tandem mass spectrometry, by using UPLC/QTOF-MS in combination with pretreatment methods. The method solves the problems such as absence of detection methods for some antidepressants in existing detection methods, high time consumption of sample pretreatment experiments, high detection costs, and low resolution of detection methods. According to the method, the recovery rate of antidepressants is 63.13-115.66%, and the limit of detection of the method is 0.14-13.47 ng/g; only 0.5 g of dry sludge sample is required. The experimental process is simplified, the introduced random errors are reduced, and the time and detection cost consumed by the experiment are greatly reduced.

公开(公告)号：US20240319053A1

公开(公告)日：2024-09-26

申请号：US18606189

申请日：2024-03-15

申请人： The University of Toledo

发明人： Shi-He Liu

IPC分类号： G01N1/34 ,  G01N1/14 ,  G01N35/02

CPC分类号： G01N1/34 ,  G01N1/14 ,  G01N35/025

摘要： An automatic fraction collector, methods of using the same, and systems including the same are described. The automatic fraction collector includes a sensor assembly able to detect liquid drops passing therethrough with light, where the sensor assembly is in the fluid flow path between a column and the point of collection of the liquid drops.

公开(公告)号：US12099051B2

公开(公告)日：2024-09-24

申请号：US18371493

申请日：2023-09-22

申请人： SOUTHWEST UNIVERSITY

发明人： Hongyuan Zhou ,  Liang Ma ,  Yuhao Zhang

IPC分类号： G01N33/00 ,  G01N1/34 ,  G01N33/50

CPC分类号： G01N33/5014 ,  G01N1/34 ,  G01N33/5085 ,  G01N2333/43534 ,  G01N2500/10

摘要： A method for establishing a biological model on joint toxicity of Caenorhabditis elegans and an application thereof are provided. The method for establishing the biological model includes adding the L1-stage Caenorhabditis elegans to a mixed system of working solution of mycotoxins containing tenuazonic acid and penicillin, K-medium solution and E. coli OP50 to obtain L1-stage Caenorhabditis elegans. The biological model indicates that TeA and PAT have a synergistic effect on the growth, development and reproductive ability of Caenorhabditis elegans, and their toxicity mechanism is related to inducing nematodes and stimulating transcriptional factors of Daf-16 genes. The biological model and detection method are simple to operate and its test period is short.

公开(公告)号：US12076666B2

公开(公告)日：2024-09-03

申请号：US17312109

申请日：2020-03-26

申请人： EMD Millipore Corporation

发明人： Akshat Gupta ,  John Paul Amara ,  Kara M. Pizzelli ,  Shannon Ryan

IPC分类号： B01D24/10 ,  B01D39/16 ,  B01D39/18 ,  B01D39/20 ,  G01N1/34 ,  G01N1/40

CPC分类号： B01D24/10 ,  B01D39/1623 ,  B01D39/18 ,  B01D39/2068 ,  G01N1/34 ,  G01N1/4077 ,  B01D2201/182 ,  G01N2001/4088

摘要： Depth filters with optimized head space are provided, as well as methods of optimizing the head space in depth filters, and methods of filtration with depth filters having optimized head space, including clarification of flocculated feed streams where pretreatment may include any of lowering cell culture pH, addition of polymers (uncharged or charged), or addition of salts to precipitate out solubilized impurities resulting in high insoluble biomass.

公开(公告)号：US20240241020A1

公开(公告)日：2024-07-18

申请号：US18246301

申请日：2021-09-23

申请人： Lonza Sales AG

发明人： Andrew WOOD ,  Michael DOHERTY ,  Aaron NOYES

IPC分类号： G01N1/34 ,  B01D15/12 ,  B01D15/18 ,  B01D15/36 ,  B01D15/38 ,  B01D15/42

CPC分类号： G01N1/34 ,  B01D15/125 ,  B01D15/1864 ,  B01D15/363 ,  B01D15/3847 ,  B01D15/424

摘要： The present disclosure relates to methods for preparing extracellular vesicles (EVs). In particular, the methods provided herein comprise contacting a sample, which comprises EVs and one or more impurities, with a depth filter, wherein the depth filter is selected from a LA media grade depth filter, a SP media grade depth filter, or both. In some aspects, the methods further comprise one or more chromatography steps. The methods enable preparation of EVs for therapeutic and diagnostic applications, and isolation and/or sub-fractionation of EVs with desired properties for specific use.

公开(公告)号：US20240183765A1

公开(公告)日：2024-06-06

申请号：US18490160

申请日：2023-10-19

申请人： City University of Hong Kong

发明人： Ting-Hsuan Chen ,  Hogi Hartanto

IPC分类号： G01N1/40 ,  G01N1/34

CPC分类号： G01N1/4005 ,  G01N1/34 ,  G01N2001/4016

摘要： A microfluidic device contains a blood sample inlet, a separation membrane, a manifold inlet in fluid connection with the blood sample inlet at a junction, a capillary pump in fluid connection with and downstream of the junction, and an air vent in in fluid connection to the capillary pump. The separation membrane contains a top layer proximal to the sample inlet, and a bottom layer distal to the sample inlet. The top layer has an average pore size of from about 1 μm to about 50 μm in diameter. The bottom layer has an average pore size of from about 0.05 μm to about 20 μm in diameter. The separation membrane is positioned between the blood sample inlet and the junction.

公开(公告)号：US20240180444A1

公开(公告)日：2024-06-06

申请号：US18547739

申请日：2021-02-25

申请人： Research Center for Eco-Environmental Sciences, Chinese Academay of Sciences

发明人： Ligang Hu ,  Chen Tao ,  Guibin Jiang

IPC分类号： A61B5/083 ,  A61B5/113 ,  G01N1/34 ,  G01N1/40 ,  G01N33/497

CPC分类号： A61B5/083 ,  A61B5/113 ,  G01N1/34 ,  G01N1/405 ,  G01N33/497 ,  A61B2503/40 ,  A61B2503/42 ,  G01N2033/4975

摘要： Provided is a system of measuring exhalation metabolism, in which: a first end of an air pump is connected to an outlet of an organic steam micro-injection module, and a second end of the air pump is connected to a first end of a liner; an air outlet of an individual exhalation imprinting sampling module is connected to a second end of the liner, and a stirrer adsorption extraction rod is provided inside the liner to sample an exhalation metabolite of an experimental animal; the air outlet of the individual exhalation imprinting sampling module is connected to a nozzle interface of an aerosol nozzle, while an air inlet thereof is connected to an air outlet of a zero-level air purifier. The system further includes a gas solenoid valve and an automatic solvent desorption module.

公开(公告)号：US20240159687A1

公开(公告)日：2024-05-16

申请号：US18281406

申请日：2022-03-25

申请人： Toray Industries, Inc.

发明人： Kenji Saito ,  Koji Nakatsuji ,  Hiroo Takabatake ,  Shinya Shimoda

IPC分类号： G01N21/95 ,  B01D61/02 ,  B01D61/10 ,  B01D63/10 ,  B01D65/10 ,  G01N1/34

CPC分类号： G01N21/95 ,  B01D61/025 ,  B01D61/10 ,  B01D63/10 ,  B01D65/109 ,  G01N1/34

摘要： The present invention relates to a damage inspection method for a reverse osmosis membrane, in which at least one of: presence or absence of physical damage; and a degree of physical damage in a reverse osmosis membrane is inspected based on presence or absence of a stained area on at least a permeate side of a stained membrane obtained by supplying a water-to-be-treated including a staining agent to the reverse osmosis membrane to stain the reverse osmosis membrane.

公开(公告)号：US20240133901A1

公开(公告)日：2024-04-25

申请号：US18278756

申请日：2022-02-24

申请人： FUJIFILM Wako Pure Chemical Corporation

发明人： Masaaki KOJIMA ,  Fumie AKUTSU

IPC分类号： G01N33/74 ,  G01N1/34

CPC分类号： G01N33/74 ,  G01N1/34 ,  G01N2410/04

摘要： An object of the present invention is to provide a simple purifying method, measuring method, and kit. The present invention is an oxytocin purifying method including treating a sample with an acid or a salt thereof, and treating the sample treated with the acid or the salt thereof with a hydrophobic carrier, and related to the purifying method, measuring method including the purifying method, and kit, in which the acid or the salt thereof is at least one or more acids or salts thereof selected from the group consisting of hydrochloric acid, acetic acid, sulfuric acid, sodium hydrogensulfate, potassium hydrogensulfate, lithium hydrogensulfate, ammonium sulfate, and a mixture thereof, and the hydrophobic carrier is a hydrophobic carrier having at least one or more functional groups on the surface, selected from the group consisting of an alkyl group having 4 to 6 carbon atoms, an alkylene glycol group, and a phenyl group.

公开(公告)号：US20240125771A1

公开(公告)日：2024-04-18

申请号：US18358320

申请日：2023-07-25

申请人： National Taiwan University ,  National Taiwan University Hospital ,  Academia Sinica

发明人： An-Bang Wang ,  Shih-Yu Chen ,  Tung-Hung Su ,  Chia-Chi Chu ,  Chia-Chien Yen ,  Yu-Wei Chiang

IPC分类号： G01N33/53 ,  G01N1/31 ,  G01N1/34

CPC分类号： G01N33/5304 ,  G01N1/31 ,  G01N1/34 ,  G01N2474/20

摘要： The present invention relates to a reaction platform, which comprises: a machine body with a bottom plate for placing non-porous substrates; and a coater module configured on the top of the machine body and capable of maintaining a preset of a predetermined height for moving along the surface of non-porous substrate, wherein the coater module has one or more slits, and a target liquid can be directly injected or sucking in from the outside of the coater module through the slit, and spreading the target liquid onto a surface of the non-porous substrate while moving along the non-porous substrate; wherein the surface of the non-porous substrate has a target to be coated. The reaction platform of the present invention can not only save time, labor and cost, but also have accurate and reproducible experimental results, showing better results than traditional methods.