公开(公告)号：US20250067749A1

公开(公告)日：2025-02-27

申请号：US18722154

申请日：2023-04-28

Applicant: PEKING UNIVERSITY

Inventor： Zhixing CHEN ,  Li Quan ,  Cong Li ,  Kunlun Cheng ,  Qijin Zhao

IPC: G01N33/68 ,  C07C249/02 ,  C07C251/24 ,  C07D215/14 ,  G01N33/53 ,  G01N33/531

Abstract: A diazo compound and a preparation method and a use thereof. The diazo compound has the structure as shown in formula wherein R1 represents H, alkyl, halogen, alkoxy or alkylamino; and R2 represents an aromatic group. On the basis of the aforementioned diazo compound as a derivatization reagent, the derivatization treatment of a small molecule carboxylic acid can significantly enhance the mass spectrometry response thereof and improve the detection sensitivity, thereby improving the detection accuracy. Moreover, it is not required to configure special chromatographic columns or special mobile phases for the derivatized small molecule carboxylic acid, and the best separation effect in a shorter time can be achieved on the basis of a lower cost, which is more conducive to high-throughput sample detection and has better detection accuracy.

公开(公告)号：US20250067701A1

公开(公告)日：2025-02-27

申请号：US18723277

申请日：2023-01-26

Applicant: BIOSENSING S.R.L.

Inventor： Bruno BOTTA ,  Francesca GHIRGA ,  Deborah QUAGLIO ,  Andrea CALCATERRA ,  Cristina DEL PLATO ,  Franco MAZZEI ,  Francesca POLLI

IPC: G01N27/327 ,  C07C59/72 ,  C07C69/712 ,  G01N27/30 ,  G01N33/53 ,  G01N33/543

Abstract: The present invention relates to novel resorc[4]arenes, such as pre-organized structure linkers for the development of high performance electrochemical biosensors.

公开(公告)号：US12235268B2

公开(公告)日：2025-02-25

申请号：US16097791

申请日：2017-06-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  BENAROYA RESEARCH INSTITUTE AT VIRGINIA MASON

Inventor： Joshua LaBaer ,  Ji Qiu ,  Kailash Karthikeyan ,  Jane Buckner ,  Gerald Nepom

IPC: G01N33/53 ,  G01N33/564 ,  G01N33/68

Abstract: Compositions and methods for detection of anti-citrullinated protein antibodies (ACPAs) in rheumatoid arthritis (RA) patients. Patient samples known or suspected of containing ACPAs were probed against citrullinated proteins, and antibody responses to 190 citrullinated proteins in 20 RA patients were investigated. Unique antibody reactivity patterns in both clinical anti-cyclic citrullinated peptide assay positive (CCP+) and negative (CCP−) RA patients were observed. At individual antigen levels, six novel antibody/antigen complexes were discovered and validated against specific citrullinated antigens (Myelin Basic Protein (MBP), osteopontin (SPP1), flap endonuclease (FENI), insulin like growth factor binding protein 6 (IGFBP6), insulin like growth factor I (IGF1) and stanniocalcin-2 (STC2)) in RA patients. Identification of immune-dominant epitope(s) for citrullinated MBP was also performed. The identified biomarkers have high specificity, especially MBP.

公开(公告)号：US12235266B2

公开(公告)日：2025-02-25

申请号：US16401418

申请日：2019-05-02

Applicant: The Government of the United States of America, as represented by the Secretary of the Navy

Inventor： David A. Kidwell ,  Shawn P. Mulvaney

IPC: G01N33/543 ,  G01N21/78 ,  G01N33/53 ,  G01N33/531 ,  G01N33/533 ,  G01N33/58 ,  B82Y99/00 ,  G01N21/77

Abstract: The present invention provides a method for preparing catalytic particles and using them for increased sensitivity in lateral flow immunoassays. Palladium salt is reduced in the presence of a protein using sodium borohydride as a reducing agent to form brown or gray particles. These particles catalytically develop a dye to detect the presence of an analyte.

公开(公告)号：US12233415B2

公开(公告)日：2025-02-25

申请号：US17417306

申请日：2019-04-29

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Si-lam Choy ,  Hilary Ely ,  John Lahmann

IPC: B01L3/00 ,  A61L27/16 ,  A61L27/56 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B01L7/00 ,  B01L9/00 ,  B23Q17/24 ,  C07K14/705 ,  C08F220/56 ,  C08L33/26 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N1/31 ,  G01N21/17 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N21/77 ,  G01N21/78 ,  G01N27/414 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74 ,  G01N35/00 ,  G01N35/10 ,  G06T7/00 ,  G06T7/90 ,  H10K10/46 ,  H10K85/00 ,  H10K85/20

Abstract: Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.

公开(公告)号：US12233184B2

公开(公告)日：2025-02-25

申请号：US17144551

申请日：2021-01-08

Applicant: Massachusetts Institute of Technology

Inventor： Edward Stuart Boyden ,  Nikita Obidin ,  Ruixuan Gao ,  Linyi Gao

IPC: B01L3/00 ,  A61L27/16 ,  A61L27/56 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B01L9/00 ,  B23Q17/24 ,  C07K14/705 ,  C08F220/56 ,  C08L33/26 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/686 ,  G01N1/31 ,  G01N21/17 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N21/77 ,  G01N21/78 ,  G01N27/414 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74 ,  G01N35/00 ,  G01N35/10 ,  G06T7/00 ,  G06T7/90 ,  H10K10/46 ,  H10K85/00 ,  H10K85/20

Abstract: The invention provides a method for preparing an expanded cell or tissue sample suitable for microscopic analysis. Expanding the sample can be achieved by binding, e.g., anchoring, key biomolecules to a DMAA-TF polymer network and swelling, or expanding, the polymer network, thereby moving the biomolecules apart as further described herein. As the biomolecules are anchored to the polymer network isotropic expansion of the polymer network retains the spatial orientation of the biomolecules resulting in an expanded, or enlarged, sample.

公开(公告)号：US20250060361A1

公开(公告)日：2025-02-20

申请号：US18938922

申请日：2024-11-06

Applicant: MESO SCALE TECHNOLOGIES, LLC.

Inventor： Anahit AGHVANYAN ,  Eli N. GLEZER ,  John KENTEN ,  George SIGAL ,  Martin STENGELIN

IPC: G01N33/53 ,  C12Q1/6804 ,  G01N21/66 ,  G01N21/76 ,  G01N21/77 ,  G01N33/533 ,  G01N33/543 ,  G01N33/569 ,  G01N33/58

Abstract: The present invention is directed to methods for improving assay specificity and performance in binding assays.

公开(公告)号：US20250059611A1

公开(公告)日：2025-02-20

申请号：US18923919

申请日：2024-10-23

Applicant: TORAY INDUSTRIES, INC. ,  NATIONAL CANCER CENTER

Inventor： Junpei KAWAUCHI ,  Hitoshi NOBUMASA ,  Satoko KOZONO ,  Satoshi KONDOU ,  Hiroko SUDO ,  Atsushi OCHIAI ,  Motohiro KOJIMA

IPC: C12Q1/6886 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00

Abstract: The present invention provides a kit or device for the detection of biliary tract cancer, and a method for detecting biliary tract cancer. The present invention relates to a kit or device for the detection of biliary tract cancer, comprising a nucleic acid capable of specifically binding to miRNA in a sample of a subject, and a method for detecting biliary tract cancer, comprising measuring the miRNA in vitro.

公开(公告)号：US20250059590A1

公开(公告)日：2025-02-20

申请号：US18936042

申请日：2024-11-04

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N33/53

Abstract: Methods of analyzing nucleic acids of a cell are provided.

公开(公告)号：US12226777B2

公开(公告)日：2025-02-18

申请号：US17415442

申请日：2019-12-16

Applicant: AMGEN INC.

Inventor： Graham F. Milne ,  Tony Y. Wang

IPC: B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B01L9/00 ,  B23Q17/24 ,  C07K14/705 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/686 ,  G01N21/17 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N21/77 ,  G01N21/78 ,  G01N27/414 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/74 ,  G01N35/00 ,  G01N35/10 ,  G06T7/00 ,  G06T7/90 ,  H10K10/46 ,  H10K85/00 ,  H10K85/20

Abstract: A well plate cover includes a base defining a base plane, and a plurality of insertion elements. At least a portion of each of the insertion elements is transparent. Each of the insertion elements is coupled to the base, and extends, in a direction orthogonal to the base plane, from the base to a distal end surface of the insertion element. The distal end surface of each of the insertion elements includes an apex that, when the respective insertion element is inserted into a well of a well plate, extends further into the well than any other portion of the distal end surface. The apex is a point, a line, or a plane having a diameter that is less than one half of a maximum diameter of the distal end surface.