公开(公告)号：US20250059577A1

公开(公告)日：2025-02-20

申请号：US18721137

申请日：2022-12-14

Applicant: TOYOBO CO., LTD. ,  SHIZUOKA PREFECTURAL UNIVERSITY CORPORATION

Inventor： Sho YOKOE ,  Kensuke OCHI ,  Shogo NAKANO ,  Sohei ITO

IPC: C12P19/34 ,  C12N9/12

Abstract: Provided is a novel reverse transcriptase with excellent thermal stability. A reverse transcriptase comprising the following amino acid sequence: (a) an amino acid sequence having at least 75% identity with the amino acid sequence represented by SEQ ID NO: 2 or 3; or (b) an amino acid sequence having deletion, substitution, insertion, or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO: 2 or 3.

公开(公告)号：US12227795B2

公开(公告)日：2025-02-18

申请号：US17592435

申请日：2022-02-03

Applicant: President and Fellows of Harvard College

Inventor： Jocelyn Yoshiko Kishi ,  Thomas E. Schaus ,  Peng Yin ,  Feng Xuan ,  Nikhil Gopalkrishnan ,  Sungwook Woo

IPC: C12Q1/68 ,  C12N15/113 ,  C12N15/64 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6825 ,  C12Q1/6853

Abstract: The present disclosure provides, in some aspects, nucleic acid-based molecular tools that enable the recording of molecular structure and soluble signals as well as the programmed assembly of molecular structures.

公开(公告)号：US20250051784A1

公开(公告)日：2025-02-13

申请号：US18926665

申请日：2024-10-25

Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHA

Inventor： Atsushi OHTA ,  Yusuke YAMAGISHI ,  Atsushi MATSUO

IPC: C12N15/70 ,  C12N5/10 ,  C12N9/00 ,  C12N9/22 ,  C12N15/09 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02

Abstract: The present invention provides modified arninoacyl-tRNA synthetases (ARSs) having increased reactivity with N-methyl amino acids compared to natural aminoacyl-tRNA synthetases. The modified aminoacyI-tRNA synthetases according to the present invention can aminoacylate tRNAs with their corresponding N-methyl-substituted amino acids such as N-methyl-phenylalanine, N-methyl-valine, N-methyl-serine, N-methyl-threonine, N-methyl-tryptophan, and N-methyl-leucine more efficiently than natural aminoacyl-tRNA synthetases. The present invention enables a more efficient production of polypeptides containing N-methyl amino acids.

公开(公告)号：US20250034611A1

公开(公告)日：2025-01-30

申请号：US18740403

申请日：2024-06-11

Applicant: Applied DNA Sciences, Inc.

Inventor： Yuhua Sun ,  Minghwa Ben Liang ,  Huan Dong ,  Qiang Zhao

IPC: C12P19/34 ,  C12N9/12

Abstract: The present application provides improved methods and compositions for in vitro transcription of mRNA that has reduced levels of dsRNA contaminant.

公开(公告)号：US20250027129A1

公开(公告)日：2025-01-23

申请号：US18478642

申请日：2023-09-29

Applicant: Seagate Technology LLC

Inventor： Riyan Alex MENDONSA ,  Gemma Roselle MENDONSA ,  Thomas Young CHANG

IPC: C12P19/34 ,  B01J19/00

Abstract: A method for serial and contemporaneous synthesis of disparate deoxy-ribonucleic acid (DNA) strands in an array of wells defined within a substrate. Each well in the array of wells contains a precursor nucleotide chain. A first subset of wells of the array of wells is designated not to receive a nucleotide of a specified nucleobase type and the first subset of wells is closed. A solution of a binding reaction enzyme and nucleotides of the specified nucleobase type bound with a corresponding chemical blocker is flowed over the array of wells. Nucleotides of the specified nucleobase type are received in each of a second subset of wells of the array of wells that are open and designated to receive the nucleotide of the specified nucleobase type. Received nucleotides of the specified nucleobase type are bound with assistance of the binding reaction enzyme to corresponding precursor nucleotide chains in the second subset of wells.

公开(公告)号：US20250027107A1

公开(公告)日：2025-01-23

申请号：US18702197

申请日：2022-10-18

Applicant: FLAGSHIP PIONEERING INNOVATIONS VII, LLC

Inventor： Alexandra Rachael Sneider ,  Jacob Rosenblum Rubens ,  Narahari Subbanna Pujar ,  Molly Krisann Gibson ,  Benjamin Andrew Portney ,  Geoffrey A. von Maltzahn ,  Camilo Ayala Breton

IPC: C12N15/85 ,  A61K9/51 ,  A61K38/21 ,  A61K48/00 ,  C12N15/113 ,  C12P19/34

Abstract: The disclosure provides, for example, single stranded, covalently closed DNA that does not form a double stranded structure longer than 100 base pairs. The ssDNA may encode an effector sequence, for instance a therapeutic protein. The ssDNA may comprise a nuclear targeting sequence (NTS). In some embodiments, the ssDNA shows decreased activation of the innate immune system compared to an otherwise similar dsDNA.

公开(公告)号：US20250026779A1

公开(公告)日：2025-01-23

申请号：US18759011

申请日：2024-06-28

Applicant: NUTCRACKER THERAPEUTICS, INC.

Inventor： Colin James McKinlay ,  Wan Lu Woo-Derleth

IPC: C07H21/02 ,  A61K31/7105 ,  C12P19/34

Abstract: Provided herein are compounds that are cap analogs for polynucleotides, e.g., RNA molecules, such as mRNA molecules. Also provided are capped polynucleotides, e.g., capped RNA molecules, such as capped mRNA molecules, wherein the 5′ end of the RNA molecule comprises a cap analog disclosed herein, drug products comprising the capped RNA molecules, methods for making capped polynucleotides disclosed herein, and kits for making the capped polynucleotides.

公开(公告)号：US12203120B2

公开(公告)日：2025-01-21

申请号：US18430378

申请日：2024-02-01

Applicant: Guangzhou Geneseed Biotech Co., Ltd.

Inventor： Ming Liu ,  Maolei Zhang ,  Yesheng Wang ,  Qiujie Cai ,  Wanjun Zhang ,  Xiaodan Ma

IPC: C12N15/11 ,  C12N15/64 ,  C12N15/72 ,  C12P19/34

Abstract: The present invention relates to the technical field of nucleic acids, and in particular, to a method for precisely preparing a circular RNA with an Anabaena intron self-cleaving ribozyme. By redesigning a target nucleic acid sequence of a linear RNA, selecting a site suitable for precise cleavage by the intron self-cleaving ribozyme, and removing exon sequences from the Anabaena intron self-cleaving ribozyme, precise cyclization of RNAs after in vitro transcription is achieved. The method provided by the present invention solves the problem in the prior art that linear RNAs cannot be precisely cyclized during the process of generating circular RNAs in vitro, and provides an ideal in vitro preparation method for circular RNA applications.

公开(公告)号：US20250019675A1

公开(公告)日：2025-01-16

申请号：US18684923

申请日：2022-08-30

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Brent M. DORR ,  Joel MELBY ,  Deepak RUDRAPPA ,  Jeliazko JELIAZKOV ,  Nilesh VAIDYA

IPC: C12N9/12 ,  C12P19/34

Abstract: The present disclosure provides T7 RNA polymerase variants, the use of which increases transcription efficiency and improves the quality and yield of the enzyme. Also provided are polynucleotides encoding the T7 RNA polymerase variants, host cells capable of expressing the T7 RNA polymerase variants, and methods of using the T7 RNA polymerase variants for high-efficiency transcription.

公开(公告)号：US12195798B2

公开(公告)日：2025-01-14

申请号：US17035436

申请日：2020-09-28

Applicant: Illumina, Inc. ,  The Regents of the University of California

Inventor： Jian-Bing Fan ,  Kun Zhang

IPC: C12P19/34 ,  C12Q1/6841 ,  C12Q1/6874

Abstract: Presented are methods and compositions for obtaining sequence information from one or more individual cells. The methods are useful for obtaining sequence information for a single nucleotide sequence, and for multiplex generation of sequence information from one or more individual cells.