公开(公告)号：US20250019675A1

公开(公告)日：2025-01-16

申请号：US18684923

申请日：2022-08-30

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Brent M. DORR ,  Joel MELBY ,  Deepak RUDRAPPA ,  Jeliazko JELIAZKOV ,  Nilesh VAIDYA

IPC: C12N9/12 ,  C12P19/34

Abstract: The present disclosure provides T7 RNA polymerase variants, the use of which increases transcription efficiency and improves the quality and yield of the enzyme. Also provided are polynucleotides encoding the T7 RNA polymerase variants, host cells capable of expressing the T7 RNA polymerase variants, and methods of using the T7 RNA polymerase variants for high-efficiency transcription.