公开(公告)号：US20240352545A1

公开(公告)日：2024-10-24

申请号：US18749700

申请日：2024-06-21

申请人： Gen-Probe Incorporated

发明人： Ankur Shah ,  Meghna Yadav

IPC分类号： C12Q1/70 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

CPC分类号： C12Q1/701 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

摘要： Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.

公开(公告)号：US20240336985A1

公开(公告)日：2024-10-10

申请号：US18749697

申请日：2024-06-21

申请人： Gen-Prob Incorporated

发明人： Ankur Shah ,  Meghna Yadav

IPC分类号： C12Q1/70 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

CPC分类号： C12Q1/701 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6853

摘要： Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.

公开(公告)号：US20240318230A1

公开(公告)日：2024-09-26

申请号：US18442591

申请日：2024-02-15

申请人： Gen-Probe Incorporated

发明人： Mehrdad MAJLESSI ,  Pamela DOUGLASS ,  Daniel KOLK

IPC分类号： C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q1/70

CPC分类号： C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q1/701 ,  C12Q2600/118 ,  C12Q2600/166

摘要： Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.

公开(公告)号：US20240309440A1

公开(公告)日：2024-09-19

申请号：US18434556

申请日：2024-02-06

申请人： The Regents of the University of California

发明人： Minnie M. Sarwal ,  Tara K. Sigdel ,  Joshua Y. Yang

IPC分类号： C12Q1/6853 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q1/6883 ,  G01N33/53 ,  G01N33/68 ,  G01N33/70 ,  G16H10/40

CPC分类号： C12Q1/6853 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q1/6883 ,  G01N33/53 ,  G01N33/68 ,  G01N33/6827 ,  G01N33/6839 ,  G16H10/40 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/158 ,  G01N33/70 ,  G01N2800/245 ,  G01N2800/347

摘要： This present disclosure provides methods and compositions that can be used to quantify cfDNA in biofluids using a hybridization approach.

公开(公告)号：US12084724B2

公开(公告)日：2024-09-10

申请号：US17494669

申请日：2021-10-05

申请人： Inivata Ltd.

发明人： Samuel Woodhouse ,  Stefanie Lensing ,  Tim Forshew ,  Vincent Plagnol ,  Matthew Edward Smith ,  Karen Howarth ,  Michael Epstein

IPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/686 ,  G16B20/00 ,  G16B30/00

CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/16 ,  G16B20/00 ,  G16B30/00 ,  C12Q1/6853 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2537/159

摘要： The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.

公开(公告)号：US12077811B2

公开(公告)日：2024-09-03

申请号：US17169145

申请日：2021-02-05

申请人： President and Fellows of Harvard College

发明人： David Yu Zhang ,  Peng Yin

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/686

CPC分类号： C12Q1/6853 ,  C07H21/02 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2525/161 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q1/6848 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q1/6848 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2525/161

摘要： Provided herein are primers and primer systems having improved specificity and kinetics over existing primers, and methods of use thereof.

公开(公告)号：US20240279713A1

公开(公告)日：2024-08-22

申请号：US18470565

申请日：2023-09-20

申请人： SIGMA-ALDRICH CO. LLC

发明人： Brian Ward ,  Kenneth Heuermann

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/6876

CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/179

摘要： The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.

公开(公告)号：US20240229129A1

公开(公告)日：2024-07-11

申请号：US18622453

申请日：2024-03-29

申请人： Synthego Corporation

发明人： Timothy Hsiau ,  Richard Stoner ,  Travis Maures ,  David Conant

IPC分类号： C12Q1/6869 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6806 ,  C12Q1/6853 ,  G01N33/487 ,  G06F17/18 ,  B01L3/00

CPC分类号： C12Q1/6869 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6806 ,  C12Q1/6853 ,  G01N33/48721 ,  G06F17/18 ,  B01L3/502761 ,  B01L2200/10 ,  C12N2310/20 ,  C12Q2563/116

摘要： The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.

公开(公告)号：US12024742B2

公开(公告)日：2024-07-02

申请号：US17558994

申请日：2021-12-22

申请人： Integrated DNA Technologies, Inc.

发明人： Joseph Dobosy ,  John Froehlig, Jr. ,  Katherine Perschbacher ,  Kristin Beltz ,  Scott Rose ,  Mark Aaron Behlke

IPC分类号： C12Q1/6848 ,  C12N9/22 ,  C12Q1/6853 ,  C12Q1/686

CPC分类号： C12Q1/6848 ,  C12N9/22 ,  C12Y301/26004 ,  C12Q1/6853 ,  C12Q1/686

摘要： The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

公开(公告)号：US20240209428A1

公开(公告)日：2024-06-27

申请号：US18426132

申请日：2024-01-29

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Qiang Liu

IPC分类号： C12Q1/6853

CPC分类号： C12Q1/6853 ,  C12Q2600/16

摘要： A new fluorescence detection method called pyrophosphorolysis activated fluorescence was developed to measure PAP amplification of nucleic acid. A fluorophore-quencher-dual-labeled blocked primer was used for PAP: I) which has a fluorophore attached to a nucleotide in the internal region or at the 5′ end and a quencher attached to a blocked nucleotide at the 3′ end, or II) which has a quencher attached to a nucleotide in the internal region or at the 5′ end and a fluorophore attached to a blocked nucleotide at the 3′ end. Multiple fluorophore-quencher-dual-labeled blocked primers were also used for multiplex PAP, which are attached with different fluorophores to distinguish multiple templates in a reaction.