公开(公告)号：US12129514B2

公开(公告)日：2024-10-29

申请号：US13934093

申请日：2013-07-02

申请人： Molecular Loop Biosolutions, LLC

发明人： Gregory J. Porreca ,  Mark Umbarger

IPC分类号： C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886

CPC分类号： C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q2537/159 ,  C12Q2600/156 ,  C12Q1/6827 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2537/162 ,  C12Q1/6827 ,  C12Q2525/307 ,  C12Q2535/122 ,  C12Q2537/159

摘要： Aspects of the invention relates to methods and compositions that are useful to reduce bias and increase the reproducibility of multiplex analysis of genetic loci. In some configurations, predetermined preparative steps and/or nucleic acid sequence analysis techniques are used in multiplex analyses for a plurality of genetic loci in a plurality of samples.

公开(公告)号：US20240264142A1

公开(公告)日：2024-08-08

申请号：US18535222

申请日：2023-12-11

申请人： Roche Sequencing Solutions, Inc.

发明人： Randall Davis

IPC分类号： G01N33/487 ,  C12Q1/6806 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686

CPC分类号： G01N33/48721 ,  C12Q1/6806 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2600/16

摘要： Provided herein are methods and compositions for detecting and/or quantitating target analytes, including nucleic acids and polypeptides, using nanopore detectable barcodes.

公开(公告)号：US20240247303A1

公开(公告)日：2024-07-25

申请号：US18392743

申请日：2023-12-21

申请人： 10x Genomics, Inc.

发明人： Katherine PFEIFFER ,  Zahra Kamila BELHOCINE

IPC分类号： C12Q1/6813 ,  C12Q1/6876

CPC分类号： C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/16

摘要： Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. The methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240209343A1

公开(公告)日：2024-06-27

申请号：US18395294

申请日：2023-12-22

申请人： Codexis, Inc.

发明人： Rachelle Copeland ,  David Elgart ,  Jason Fell ,  Jovana Nazor ,  Leland Ken Wong ,  Zhe Rui

IPC分类号： C12N9/00 ,  C12Q1/6813

CPC分类号： C12N9/93 ,  C12Q1/6813 ,  C12Y605/01003

摘要： The present disclosure relates to engineered RNA ligase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered RNA ligase polypeptides. The present disclosure also provides methods of using the engineered RNA ligase polypeptides or compositions thereof for molecular biological, diagnostic, and other purposes.

公开(公告)号：US11992515B2

公开(公告)日：2024-05-28

申请号：US17080268

申请日：2020-10-26

申请人： KING FAISAL SPECIALIST HOSPITAL & RESEARCH CENTRE

发明人： Eid Abdullah Al Mutairy ,  Mohammed Khalid ,  Futwan Al-Mohanna

IPC分类号： A61P11/00 ,  A61K9/00 ,  A61K38/00 ,  A61K38/17 ,  A61P9/04 ,  A61P11/08 ,  C07K14/47 ,  C12N15/52 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6827

CPC分类号： A61K38/1738 ,  A61P9/04 ,  A61P11/00 ,  C07K14/4728 ,  C12N15/52 ,  A61K9/0073 ,  A61K9/0075 ,  C12Q1/6813 ,  C12Q1/6827

摘要： The invention is directed to a method for diagnosing and treating a pulmonary lung disease by detecting a mutant S100A3 protein associated with pulmonary lung disease and by treating a subject with a functional S100A3 protein.

公开(公告)号：US20240150834A1

公开(公告)日：2024-05-09

申请号：US17980393

申请日：2022-11-03

申请人： Roche Sequencing Solutions, Inc.

发明人： Eva MARTIN ,  Florian RUBELT

IPC分类号： C12Q1/6881 ,  C12N15/10 ,  C12Q1/6813 ,  C12Q1/6844

CPC分类号： C12Q1/6881 ,  C12N15/1065 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q2600/112

摘要： Methods of monitoring the development of FNAIT using immune profiling is described.

公开(公告)号：US11851712B2

公开(公告)日：2023-12-26

申请号：US16978758

申请日：2019-03-06

申请人： Board of Regents, The University of Texas System

发明人： Daniel McGrail ,  Shiaw-Yih Lin ,  Patrick Pilie ,  Eric Jonasch ,  Curtis Chun-Jen Lin

IPC分类号： C12Q1/6886 ,  C12Q1/6813

CPC分类号： C12Q1/6886 ,  C12Q1/6813 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/158

摘要： Methods for increasing immune therapy response and predicting likelihood of cancer metastasis by analyzing the expression of genes associated with replication stress response. In some aspects, cancers are treated with immune checkpoint inhibitors and/or MEK inhibitors. Methods for selecting patients by analyzing the expression of genes associated with a defect in replication stress response are also provided.

公开(公告)号：US20230392201A1

公开(公告)日：2023-12-07

申请号：US18330279

申请日：2023-06-06

申请人： Element Biosciences, Inc.

发明人： James A. STAPLETON ,  Timothy WHITEHEAD ,  Michael PREVITE ,  Molly HE ,  Tuval BEN-YEHEZKEL ,  Matthew KELLINGER ,  Kyle METCALFE

IPC分类号： C12Q1/6869 ,  C12Q1/686 ,  C12N15/10 ,  C12Q1/6813

CPC分类号： C12Q1/6869 ,  C12Q1/686 ,  C12N15/1065 ,  C12Q1/6813

摘要： The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

公开(公告)号：US11828755B2

公开(公告)日：2023-11-28

申请号：US16307125

申请日：2017-06-07

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Benjamin Ming Wu ,  Daniel William Bradbury ,  Shin Ting Sherine Frieda Cheung

IPC分类号： G01N33/543 ,  C12Q1/6844 ,  C12Q1/6813

CPC分类号： G01N33/54388 ,  C12Q1/6813 ,  C12Q1/6844 ,  G01N33/543 ,  G01N33/54306 ,  C12Q1/6813 ,  C12Q2563/159 ,  C12Q2565/625 ,  C12Q1/6844 ,  C12Q2521/513 ,  C12Q2531/119 ,  C12Q2531/143 ,  C12Q2563/159

摘要： In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.

公开(公告)号：US11795497B2

公开(公告)日：2023-10-24

申请号：US17227121

申请日：2021-04-09

申请人： Quantum-Si Incorporated

发明人： Andrea Marziali ,  Milenko Despotovic ,  Joel Pel

IPC分类号： C12Q1/6869 ,  B03C5/02 ,  C12Q1/6832 ,  C12Q1/6837 ,  C12Q1/6825 ,  B01L3/00 ,  C12N15/10 ,  B01D29/05 ,  B01D35/027 ,  B62D5/06 ,  F15B1/26 ,  F15B21/041 ,  B03C5/00 ,  B01L7/00 ,  C12Q1/6813

CPC分类号： C12Q1/6832 ,  B01D29/05 ,  B01D35/027 ,  B01D35/0273 ,  B01L3/502753 ,  B03C5/005 ,  B03C5/026 ,  B62D5/062 ,  C12N15/101 ,  C12Q1/6825 ,  C12Q1/6837 ,  F15B1/26 ,  F15B21/041 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2300/1805 ,  B01L2400/0421 ,  B01L2400/0451 ,  B03C2201/18 ,  B03C2201/26 ,  C12Q1/6813 ,  C12N15/101 ,  C12Q2527/101 ,  C12Q2563/107 ,  C12Q2565/125

摘要： Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.