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公开(公告)号：US20240240271A1
公开(公告)日：2024-07-18
申请号：US18625389
申请日：2024-04-03
Applicant: Gen-Probe Incorporated
Inventor： Matthias Jost , Pamela Douglass , Daniel P. Kolk , Mehrdad R. Majlessi
IPC: C12Q1/70
CPC classification number: C12Q1/701 , C12Q1/70 , C12Q2561/101 , C12Q2563/107 , C12Q2600/16
Abstract: This disclosure concerns amplification primers, hybridization assay probes, compositions containing such primers and probes, and associated reagents, kits, and methods, that can be used to analyze samples for the presence of Influenza A virus, Influenza B virus, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B target nucleic acids.
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公开(公告)号：US12018321B2
公开(公告)日：2024-06-25
申请号：US17114541
申请日：2020-12-08
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Frederic Vigneault , Kalim U. Mir
IPC: C12Q1/68 , C12N9/22 , C12N15/11 , C12Q1/6832 , C12Q1/6874
CPC classification number: C12Q1/6832 , C12N9/22 , C12N15/11 , C12Q1/6874 , C12N2310/20 , C12N2800/80 , C12Q1/6874 , C12Q2521/301 , C12Q2543/10 , C12Q2563/107 , C12Q2565/601
Abstract: Methods of detecting, probing, mapping and directed sequencing of target nucleic acids are provided using a guide RNA and a Cas9 protein. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the guide RNA includes a 3′ tail sequence that can hybridize to a probe are provided. Methods for detecting the binding of the guide RNA/Cas9 complex to a target nucleic acid where the complex is physically detected are provided.
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公开(公告)号：US11920040B2
公开(公告)日：2024-03-05
申请号：US18073929
申请日：2022-12-02
Applicant: ELITECHGROUP MDX LLC
Inventor： Eugeny A. Lukhtanov
IPC: C09B11/24 , C12N9/12 , C12Q1/68 , C12Q1/6876 , G01N21/64
CPC classification number: C09B11/24 , C12N9/1241 , C12Q1/6876 , G01N21/6486 , C12N2310/3517 , C12Q2561/101 , C12Q2563/107
Abstract: The carborhodamine dyes disclosed herein are novel reagents suitable for automated incorporation of carborhodamine dyes into oligonucleotides that can be used in detection methods for nucleic acid targets. This disclosure provides an efficient and simple process for the preparation of carborhodamine compounds and introduces previously unknown reagents for the automated synthesis of oligonucleotide-carborhodamine conjugates.
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公开(公告)号：US11911769B2
公开(公告)日：2024-02-27
申请号：US17039106
申请日：2020-09-30
Applicant: Abbott Laboratories
Inventor： Sonal Sadaria Nana , Eric B. Shain , Michael S. Hazell , Eric D. Yeaton , Michael Giraud , Timothy J. Patno , Ali Attarwalla , Dean Khan , Matthew J. Hayes
CPC classification number: B01L7/52 , B01L2200/147 , B01L2300/0609 , B01L2300/0645 , B01L2300/0654 , B01L2300/12 , B01L2300/16 , B01L2300/1822 , B01L2300/1894 , C12Q2563/103 , C12Q2563/107 , C12Q2565/60 , G01N21/93 , G01N30/74 , G01N30/8624
Abstract: The instant disclosure provides nucleic acid amplification systems and multi-reaction analysis systems useful in the efficient processing of samples, including clinical samples. Integrated systems that include nucleic acid amplification devices functionally combined with multi-reaction analysis systems are also included. Also provided are methods for monitoring multiple concurrent nucleic acid amplification reactions that include the use of devices and systems described herein.
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公开(公告)号：US20240052402A1
公开(公告)日：2024-02-15
申请号：US18467927
申请日：2023-09-15
Applicant: Drexel University
Inventor： Ming Xiao , Jennifer McCaffrey
IPC: C12Q1/6827
CPC classification number: C12Q1/6827 , C12Q2521/101 , C12Q2521/307 , C12Q2563/107
Abstract: A method of detecting the length of an individual telomere is provided. In one embodiment, the method includes contacting genomic DNA with a guide RNA having a portion complementary to a telomere repeat sequence in the genomic DNA and with Cas9 nickase to produce a single-strand break in the genomic DNA at the telomere repeat sequence. The nicked DNA is contacted with a polymerase and fluorescently labeled nucleotide, wherein the fluorescently labeled nucleotide is incorporated into the nicked DNA at the telomere repeat sequence. The genomic DNA is contacted with a second nicking endonuclease which is specific for a sequence motif in the genomic DNA thereby producing a second nick in the genomic DNA at the motif sequence. The nicked DNA is contacted with a polymerase and second fluorescently labeled nucleotide of different color, wherein the second fluorescently labeled nucleotide is incorporated into the nicked DNA at the motif sequence location. The length of the telomere is detected by measuring the fluorescence of first fluorescently labeled nucleotide at the telomere repeat location, wherein the fluorescently labeled motif sequences are used as a barcode to identify the chromosome.
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公开(公告)号：US11891658B2
公开(公告)日：2024-02-06
申请号：US17394692
申请日：2021-08-05
Applicant: Ultima Genomics, Inc.
Inventor： Gilad Almogy , Florian Oberstrass , Omer Barad , Chandan Shee
IPC: C12Q1/686
CPC classification number: C12Q1/686 , C12Q2563/107 , C12Q2563/149 , C12Q2563/159
Abstract: The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). The methods described herein can be used to achieve clonal amplification even with a greater than Poisson distribution of beads and/or nucleic acid templates into an emulsion. A PCR method may comprise generating a partition (e.g., a droplet) comprising at least two beads and/or at least two nucleic acid molecules and generating clonal amplification products corresponding to the nucleic acid molecule, at least a subset of which may be attached to a bead.
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公开(公告)号：US20230393071A1
公开(公告)日：2023-12-07
申请号：US18450746
申请日：2023-08-16
Applicant: 10x Genomics, Inc.
Inventor： Felice Alessio Bava
IPC: G01N21/64 , C12Q1/6837
CPC classification number: G01N21/6458 , C12Q1/6837 , C12Q2563/107
Abstract: This disclosure relates to compositions and methods for analyzing a tissue section from a biological sample.
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公开(公告)号：US11827932B2
公开(公告)日：2023-11-28
申请号：US17153309
申请日：2021-01-20
Applicant: Illumina, Inc.
Inventor： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/6869
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2535/122 , C12Q2563/107
Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
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公开(公告)号：US11827921B2
公开(公告)日：2023-11-28
申请号：US16864744
申请日：2020-05-01
Applicant: California Institute of Technology
Inventor： Emil P. Kartalov , Aditya Rajagopal , Axel Scherer
IPC: G01N33/48 , G01N33/50 , C12Q1/6825 , G16B25/00 , G16B5/00 , C12Q1/6851 , G16B25/20 , G16B40/10 , C12Q1/68 , G01N21/64 , G06F17/10 , G06F17/11 , G16B40/00
CPC classification number: C12Q1/6825 , C12Q1/68 , C12Q1/6851 , G01N21/6486 , G16B5/00 , G16B25/00 , G16B25/20 , G16B40/10 , G06F17/10 , G06F17/11 , G16B40/00 , Y02A50/30 , C12Q1/6851 , C12Q2537/101 , C12Q2537/143 , C12Q2537/165 , C12Q2563/107
Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
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公开(公告)号：US11718639B2
公开(公告)日：2023-08-08
申请号：US17230007
申请日：2021-04-14
Applicant: Pacific Biosciences of California, Inc.
Inventor： Keith Bjornson , Jeremiah Hanes , Erik Miller , Satwik Kamtekar , Lubomir Sebo , Louis Brogley
IPC: C07H21/04 , C07H19/06 , C07H19/16 , G01N33/58 , C07K14/36 , C12Q1/6874 , C12N9/10 , C09B23/08 , C09B69/10 , C12Q1/6869
CPC classification number: C07H21/04 , C07H19/06 , C07H19/16 , C07K14/36 , C09B23/083 , C09B69/105 , C12N9/10 , C12Q1/6869 , C12Q1/6874 , G01N33/582 , G01N33/583 , C12Q2523/313 , C12Q2535/113 , C12Q2563/107 , C12Q2563/131 , C12Q1/6874 , C12Q2523/313 , C12Q2563/107 , C12Q2563/131 , C12Q1/6869 , C12Q2535/113 , C12Q2563/107
Abstract: Compositions, methods, and systems are provided for fluorescent polymerase enzyme substrates comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Fluorescent polymerase enzyme substrates of the invention have a protein shield between the fluorescent dye moieties and nucleotide moieties of the polymerase enzyme substrate. The polymerase enzyme substrates have a nucleotide component and a dye component, each attached to a protein. The attachments can be covalent. The protein can, for example, prevent the direct interaction of the fluorescent dye moiety with the enzyme when carrying out nucleotide synthesis, preventing photodamage to the enzyme. The polymerase enzyme substrates of the invention can have multiple dyes and multiple nucleotide moieties.
