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公开(公告)号：US20240353402A1
公开(公告)日：2024-10-24
申请号：US18643170
申请日：2024-04-23
Applicant: TBP Diagnostics, LLC
Inventor： Lawrence Silbart , Thanh Nguyen
IPC: G01N33/543 , G01N33/53 , G01N33/569
CPC classification number: G01N33/54388 , G01N33/5308 , G01N33/56905 , G01N33/56911 , G01N33/56983 , G01N2333/08 , G01N2333/11 , G01N2333/183 , G01N2333/195 , G01N2333/20 , G01N2469/10
Abstract: Systems and methods are described herein for a diluent-containing tube comprising a beveled grinding surface and operable to receive a plunger comprising a complementary beveled grinding surface. Solid matrices placed within the diluent can be ground between the grinding surfaces to release molecules into the diluent for detection. The plunger may further comprise a groove containing a test strip operable to be deployed into the diluent after grinding for detection of the released molecules. In certain embodiments, the test strip may be a lateral flow immunoassay rapid antigen detection (LFA) test strip. The solid matrix may be a tick and the molecules may be antigens or pathogens related to tick-borne illnesses.
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公开(公告)号：US12123873B2
公开(公告)日：2024-10-22
申请号：US17043742
申请日：2019-04-02
Applicant: SANOFI
Inventor： Jörg-M. Hollidt , Thomas Bogusch , Franziska Werner , Hardy Kietzmann
IPC: G01N33/543
CPC classification number: G01N33/54388 , G01N33/54346 , G01N33/54393 , G01N2333/78 , G01N2800/105
Abstract: The invention relates to a lateral flow assay device for the detection of a target peptide in a liquid sample, comprising: (a) a solid support, (b) a sample pad for initially receiving and optionally pre-treating the sample at a first end of the solid support, (c) an absorbent pad at a second end of the solid support, (d) a conjugate pad comprising in a dry state a mobilizable conjugate of a particle and the target peptide, wherein the target peptide conjugated to said particle is biotinylated and the particle comprises on its surface a biotin-binding protein, (e) a target peptide reaction membrane comprising, (i) a capture region comprising an immobilized first capture reagent against the target peptide, and (ii) optionally a control region comprising an immobilized second capture reagent against particle control conjugate, wherein the sample pad, the conjugate pad, the reaction membrane and the absorbent pad are mounted to the solid support to permit capillary flow from the sample pad to the reaction membrane through the conjugate pad, and wherein the loading of the particle with the target peptide is from about 20% to about 55% of the maximal loading capacity of the particle.
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公开(公告)号：US20240345079A1
公开(公告)日：2024-10-17
申请号：US18659829
申请日：2024-05-09
Applicant: Zhejiang Orient Gene Biotech Co.,Ltd
Inventor： Guoliang YUAN , Jianqiu FANG , Siyu LEI , Kai FANG
IPC: G01N33/543
CPC classification number: G01N33/54388 , G01N2333/59
Abstract: The present invention provides a test device. The device includes a testing element and a house configured to accommodate the testing element, where the house is formed by folding a paper-made card, and the testing element is located in the housing. The housing is allowed to be in different change states to test or assay an analyte in a sample.
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4.发明授权
公开(公告)号：US12117444B2
公开(公告)日：2024-10-15
申请号：US16646892
申请日：2019-08-30
Applicant: ASAHI KASEI KABUSHIKI KAISHA
Inventor： Kotaro Oda , Katsuyoshi Takahashi , Mika Sugata , I-Nung Huang , Kazuhiko Arakawa , Yuichiro Takahashi
IPC: G01N33/553 , C07C229/12 , C12N1/06 , G01N33/543 , G01N33/547 , G01N33/558 , G01N33/58
CPC classification number: G01N33/54391 , C07C229/12 , C12N1/06 , G01N33/54388 , G01N33/547 , G01N33/553 , G01N33/58 , G01N2458/00
Abstract: Detection rapidly and with high detection intensity is accomplished while blackening is inhibited during silver enhancement to detect an analyte in a specimen. One embodiment of the present invention provides an enhancing agent to be used for silver enhancement in detection of an analyte in a specimen by metal labeling and silver enhancement, the enhancing agent including:
(a) a silver-containing compound,
(b) a silver ion-reducing agent, and
(c) a reaction rate controller,
wherein the reaction rate controller (c) is a compound selected from the group consisting of compounds represented by the following formula (I):
(wherein:
R1, R2 and R3 each independently represent a hydrogen atom or an optionally substituted monovalent aliphatic hydrocarbon group of 1 to 30 carbon atoms, with the proviso that R1, R2 and R3 are not all hydrogen atoms, or R1 and R2 form a 5-membered ring or 6-membered ring and R3 represents a hydrogen atom or an optionally substituted monovalent aliphatic hydrocarbon group of 1 to 30 carbon atoms;
X represents a divalent hydrocarbon group of 1 to 3 carbon atoms; and
n is an integer of 1 to 3).
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公开(公告)号：US20240318267A1
公开(公告)日：2024-09-26
申请号：US18188527
申请日：2023-03-23
Applicant: City University of Hong Kong
Inventor： Mengsu YANG , Bereket AYELE , Renardi GUNAWAN , Wanqing WU
IPC: C12Q1/70 , G01N33/543 , G01N33/569
CPC classification number: C12Q1/701 , G01N33/54388 , G01N33/56983 , G01N2333/065 , G01N2333/165
Abstract: The present disclosure provides a LAMP-based, one-pot pathogen detection system and method integrating a high specificity assay for identifying true positive samples in an analyte before a whole course of LAMP reaction completes, in order to shorten the reaction time and reduce the likelihood of false positive results due to non-specific amplification or signal error (e.g., sample has too high pH) in the LAMP reaction. The present invention integrates an immunochromatographic assay such as lateral flow assay (LFA) in result interpretation from colorimetric and/or fluorimetric aspects of the LAMP reaction in order to enable a real-time pathogen detection, thereby improving the efficiency thereof with high specificity. The present detection system employs a single reaction system to enable multiple result interpretation, and the LAMP reaction can be carried out in a simple platform capable of generating constant heat.
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公开(公告)号：US12099056B2
公开(公告)日：2024-09-24
申请号：US17554268
申请日：2021-12-17
Applicant: ORASURE TECHNOLOGIES, INC.
Inventor： Giffin Daughtridge , Keith Kardos , Sharanappa Basappa Rajur , Kim Hwa-Ok , Asharani Dhondiram Kadam
IPC: G01N33/53 , C07K16/44 , G01N33/543 , G01N33/577
CPC classification number: G01N33/5308 , C07K16/44 , G01N33/54388 , G01N33/577 , C07K2317/565 , G01N2430/60
Abstract: The invention provides novel compounds, reagents, systems, and methods for detecting a metabolite related to a NRTI in a biological sample, and uses thereof in monitoring adherence to pre-exposure prophylaxis. Such reagents comprise NRTI derivatives, analogs, NRTI derivatives conjugates, along with antibodies directed to same, which are useful for antibody-based methods, such as a lateral flow immunoassay and other point of care devices.
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公开(公告)号：US12078636B2
公开(公告)日：2024-09-03
申请号：US17786245
申请日：2020-12-18
Applicant: Rapid Pathogen Screening, Inc.
Inventor： Robert P Sambursky
IPC: G01N33/543
CPC classification number: G01N33/54388
Abstract: A test strip with selective white blood cell (WBC) lysis for release of WBC contents and the capture and holding of red blood cells (RBC), allowing for fast clearance and result interpretation and more accurate results at early time points due to the lack of red color interference caused by background red heme color of lysing of RBCs. The removal of the red color interference enhances both visual and digital interpretation of test strips, such as test strips of an immunoassay test. The test strip enhances the detection of both intracellular proteins of WBCs and extracellular proteins of RBCs simultaneously such as MxA and CRP, MxA and PCT, MxA and HNL, and MxA and IL-6, MxA and myeloid cells (STREM-1), MxA and angiopoietin 2, MxA and vascular endothelial growth factor (VEGF) or its soluble vascular endothelial growth factor receptor-1 (sVEGFR1), MxA and heparin binding protein (HBP) or other combinations.
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公开(公告)号：US20240272158A1
公开(公告)日：2024-08-15
申请号：US18566740
申请日：2022-06-06
Applicant: Denka Company Limited
Inventor： Koji HIRAOKA , Yuji SAITO
IPC: G01N33/569 , G01N33/53 , G01N33/543
CPC classification number: G01N33/56983 , G01N33/5308 , G01N33/54388 , G01N2333/085 , G01N2469/10
Abstract: Disclosed are a method of testing a fecal sample, which method suppresses the effect of interfering substances contained in the fecal sample in immunochromatography, to enable accurate and specific measurement of a substance to be detected in the test sample, and an immunochromatographic test piece therefor. The method of testing a fecal sample includes testing the fecal sample by immunochromatography in the presence of taurodeoxycholic acid or a salt thereof. The immunochromatographic test piece for testing a fecal sample includes taurodeoxycholic acid or a salt thereof in a sample drop region.
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9.发明授权
公开(公告)号：US12061196B2
公开(公告)日：2024-08-13
申请号：US17522696
申请日：2021-11-09
Applicant: MFB Fertility, Inc.
Inventor： Larry Joseph Koudele , Alexandra Marie Koudele
CPC classification number: G01N33/54388 , G01N21/6428 , G01N33/689 , G01N33/743 , G01N33/76 , G06T7/11 , G06T7/90 , G06T2207/20132
Abstract: An exemplary method of interpreting results indicated on a photographed diagnostic test comprises: sweeping the photographed diagnostic test, or cropped area of the photographed diagnostic test; creating a vector of color values including the color values obtained from a plurality of horizontal pixel positions on the photographed diagnostic test or cropped area of the photographed diagnostic test; identifying the location of the control line and each testing line depicted within the photographed diagnostic test; and calibrating to determine a hormone or analyte level corresponding to a color value of a test line. Systems of interpreting results indicated on a photographed diagnostic test are also provided.
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公开(公告)号：US20240255500A1
公开(公告)日：2024-08-01
申请号：US18018968
申请日：2021-07-29
Applicant: Illinois State University
Inventor： Jun-Hyun Kim , Jeremy D. Driskell
IPC: G01N33/543 , G01N21/65
CPC classification number: G01N33/54388 , G01N21/658
Abstract: A diagnostic method that includes providing a plasmonic paper and an absorbing pad positioned under the plasmonic paper, pre-immobilizing an antibody onto the plasmonic paper, introducing a sample solution to the plasmonic paper to extract and concentrate antigen in the sample on the plasmonic paper, absorbing the remainder of the sample solution with the absorbing pad, passing Extrinsic Raman Labels through the plasmonic paper to label captured antigens, and detecting captured antigen.
