公开(公告)号：US12077811B2

公开(公告)日：2024-09-03

申请号：US17169145

申请日：2021-02-05

Applicant: President and Fellows of Harvard College

Inventor： David Yu Zhang ,  Peng Yin

IPC: C12Q1/68 ,  C07H21/02 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/686

CPC classification number: C12Q1/6853 ,  C07H21/02 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2525/161 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q1/6848 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q1/6848 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2525/161

Abstract: Provided herein are primers and primer systems having improved specificity and kinetics over existing primers, and methods of use thereof.

公开(公告)号：US20240026467A1

公开(公告)日：2024-01-25

申请号：US18208624

申请日：2023-06-12

Applicant: Quest Diagnostics Investments LLC

Inventor： Lakshmi NAIR ,  Heather VINCENT ,  Huong MAI ,  Michelle TABB ,  Maurice EXNER

IPC: C12Q1/689 ,  G01N21/64

CPC classification number: C12Q1/689 ,  G01N21/6428 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2565/1015 ,  C12Q2537/143 ,  C12Q2563/107 ,  C12Q2600/112 ,  C12Q2600/16 ,  C12Q2600/158 ,  G01N2021/6432 ,  G01N2021/6441

Abstract: Disclosed are methods of identifying a methicillin-resistant Staphylococcus aureus (MRSA) in a sample wherein the methods involve detecting a S. aureus-specific nucleic acid sequence, mecA and mecC, in the sample. Kits for determining the presence of MRSA in a sample are also provided.

公开(公告)号：US11857940B2

公开(公告)日：2024-01-02

申请号：US17398865

申请日：2021-08-10

Applicant: Fluidigm Corporation

Inventor： Peilin Chen

IPC: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844 ,  B01J19/00

CPC classification number: B01J19/0046 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12Q1/6848 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US11788125B2

公开(公告)日：2023-10-17

申请号：US17324944

申请日：2021-05-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green

IPC: C07H21/02 ,  C12Q1/6841 ,  C12N15/115 ,  G01N33/53

CPC classification number: C12Q1/6841 ,  C12N15/115 ,  G01N33/5308 ,  C12N2310/16 ,  C12N2310/531 ,  C12Q2525/205 ,  C12Q2525/301

Abstract: Provided herein are imaging probes and systems and methods employing such imaging probes for real-time, label-free, multiplexed imaging of RNAs in living cells. More particularly, aptamer-based sensors (“aptasensors”) and molecular fuses comprising multiple aptasensors are genetically encoded imaging probes comprising RNA-target binding sequence and an intramolecular reconfiguration sequence. The probe is configured such that binding of a RNA target by the RNA-target binding sequence triggers the intramolecular reconfiguration sequence to reconfigure such that an optically detectable output is generated by the probe.

公开(公告)号：US11708613B2

公开(公告)日：2023-07-25

申请号：US16609994

申请日：2018-04-27

Applicant: The U.S.A., as represented by the Secretary, Department of Health and Human Services

Inventor： Amanda E. Calvert

IPC: C12Q1/6888 ,  C12Q1/70 ,  C12Q1/6844

CPC classification number: C12Q1/6888 ,  C12Q1/6844 ,  C12Q1/701 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2600/16

Abstract: A highly sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect ZIKV nucleic acid in biological samples is described. The disclosed assay is capable of detecting as few as one RNA copy per μl and can be performed in a clinical or field setting with minimal equipment and technological expertise. Oligonucleotide primers and kits for detecting ZIKV nucleic acid are also described.

公开(公告)号：US11702703B2

公开(公告)日：2023-07-18

申请号：US16759577

申请日：2018-11-06

Applicant: NantHealth Labs, Inc.

Inventor： Shahrooz Rabizadeh

IPC: C12Q1/68 ,  C12Q1/6886 ,  G16B5/00 ,  C12Q1/6806 ,  C12Q1/6853

CPC classification number: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6853 ,  G16B5/00 ,  C12Q2525/301 ,  C12Q2531/113 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: Methods of isolating cell free RNA from individual's bodily fluid and reliably obtain cell free RNA data are presented, preferably by use of high-stability portions and/or use of targeted small amplicons on the cell free RNA.

公开(公告)号：US11649486B2

公开(公告)日：2023-05-16

申请号：US16325247

申请日：2017-08-15

Applicant: Olink Bioscience AB

Inventor： Ulf Landegren ,  Johan Björkesten

IPC: C12Q1/6844 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6827

CPC classification number: C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/131 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/163 ,  C12Q1/6832 ,  C12Q2531/125 ,  C12Q2537/163

Abstract: Nucleic acid probes for detection of a target nucleic acid molecule by an RCA reaction in the presence of the target nucleic acid molecule, comprise a first circular template strand which is capable of acting as a template for RCA, and is protected from RCA in the absence of the target nucleic acid molecule by at least a second protector strand which comprises a region of complementarity to the first template strand and is hybridised thereto to form a double-stranded circular structure containing the first template strand inside the protector strand(s). At least one of the second and/or any further protector strands comprises a target binding site, such that upon binding of the probe to the target nucleic acid molecule the probe is able to undergo a strand displacement reaction which allows RCA of the first template strand. Methods of detecting target analytes use such probes.

公开(公告)号：US20180320225A1

公开(公告)日：2018-11-08

申请号：US15816979

申请日：2017-11-17

Applicant: Illumina, Inc.

Inventor： Kevin Gunderson ,  Frank Steemers

IPC: C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6837 ,  C12Q1/6816 ,  C12Q1/682

CPC classification number: C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6848 ,  C12Q2565/537 ,  C12Q2565/549 ,  C12Q2565/513 ,  C12Q2537/143 ,  C12Q2535/125 ,  C12Q2537/163 ,  C12Q2525/301 ,  C12Q2522/101

Abstract: This invention provides methods of amplifying genomic DNA to obtain an amplified representative population of genome fragments. Methods are further provided for obtaining amplified genomic DNA representations of a desired complexity. The invention further provides methods for simultaneously detecting large numbers of typable loci for an amplified representative population of genome fragments. Accordingly the methods can be used to genotype individuals on a genome-wide scale.

公开(公告)号：US20180311636A1

公开(公告)日：2018-11-01

申请号：US15927864

申请日：2018-03-21

Applicant: Gen9, Inc.

Inventor： Joseph Jacobson ,  Larry Li-Yang Chu

IPC: B01J19/00 ,  C12Q1/6837 ,  C12N15/10 ,  C12Q1/6811

CPC classification number: B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00722 ,  C12N15/1031 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q2565/525 ,  C12Q2525/301

Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

公开(公告)号：US20180274029A1

公开(公告)日：2018-09-27

申请号：US15926341

申请日：2018-03-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tal RAZ ,  Pascaline MARY

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6848

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2527/107

Abstract: The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.