公开(公告)号：US12076701B2

公开(公告)日：2024-09-03

申请号：US17161007

申请日：2021-01-28

申请人： 10X Genomics, Inc.

发明人： Felice Alessio Bava

IPC分类号： B01J19/00

CPC分类号： B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00722

摘要： Disclosed are extended and/or branched oligonucleotide capture probe assemblies for use in spatial transcriptomics systems, and methods for making the capture probe assemblies.

公开(公告)号：US20240280573A1

公开(公告)日：2024-08-22

申请号：US18469907

申请日：2023-09-19

申请人： California Institute of Technology

发明人： Rong Fan ,  Habib Ahmad ,  James R. Heath

IPC分类号： G01N33/543 ,  B01J19/00 ,  B01L3/00 ,  G01N33/58

CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01L3/502746 ,  B01L3/502753 ,  G01N33/54366 ,  G01N33/582 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N2458/10

摘要： Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

公开(公告)号：US11999948B2

公开(公告)日：2024-06-04

申请号：US15970690

申请日：2018-05-03

申请人： The Regents of the University of California

发明人： Hugh E. Olsen ,  Miten Jain ,  Mark A. Akeson

IPC分类号： C12Q1/6809 ,  C12N15/10 ,  C12N15/11 ,  C12Q1/6806 ,  C40B30/04 ,  C40B70/00 ,  C40B40/06

CPC分类号： C12N15/1065 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/11 ,  C12Q1/6806 ,  C40B30/04 ,  C40B70/00 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00759 ,  C40B40/06 ,  C12N15/1093 ,  C12Q2537/159

摘要： Provided are methods of producing size-selected nucleic acid libraries. The methods include contacting a nucleic acid sample and a nucleic acid binding reagent including an affinity tag, under conditions in which nucleic acids of less than a desired length are substantially bound to the nucleic acid binding reagent and nucleic acids of the desired length are substantially not bound to the nucleic acid binding reagent. The conditions include the duration of the contacting, the concentration of the nucleic acid binding reagent, or both. The methods further include separating, using the affinity tag, the nucleic acids of less than the desired length bound to the nucleic acid binding reagent from the nucleic acids of the desired length not bound to the nucleic acid binding reagent, to produce a size-selected nucleic acid library. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure, are also provided.

公开(公告)号：US11953464B2

公开(公告)日：2024-04-09

申请号：US17831424

申请日：2022-06-02

申请人： ILLUMINA, INC.

发明人： Dietrich Dehlinger ,  Ali Agah ,  Tracy Helen Fung ,  Emrah Kostem

IPC分类号： G01N27/414 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/64 ,  G01N21/76 ,  G06F18/24 ,  G06V10/75 ,  G16B30/00

CPC分类号： G01N27/4145 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N21/6454 ,  G06F18/24 ,  G06V10/751 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0893 ,  C12Q1/6869 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2021/6482 ,  G01N21/76 ,  G01N21/763 ,  G01N2223/40 ,  G01N2223/413 ,  G05B2219/37563 ,  G16B30/00

摘要： A device for base calling is provided. The device includes a receptacle configured to hold a biosensor having a sample surface holding a plurality of clusters during a sequence of sampling events, an array of sensors sensing information from clusters disposed in corresponding pixel areas of the sample surface during the sampling events and generate sequences of pixel signals and a communication port configured to output the sequences of pixel signals. The device also includes a signal processor coupled to the communication port and configured to receive and process at least one pixel signal in the sequences of pixel signals that mixes light gathered from at least two clusters in a corresponding pixel area, and to base call each of the at least two clusters using the at least one pixel signal.

公开(公告)号：US11931713B2

公开(公告)日：2024-03-19

申请号：US15102209

申请日：2014-11-26

申请人： Global Life Sciences Solutions Operations UK Ltd

发明人： Jeffrey Kenneth Horton ,  Peter James Tatnell ,  Robert Stone

IPC分类号： B01J19/00 ,  C12Q1/68 ,  C12Q1/6874

CPC分类号： B01J19/0046 ,  C12Q1/68 ,  C12Q1/6874 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/68 ,  C12Q2563/185

摘要： A data storage medium is disclosed comprising a solid support matrix including an optional stabilising reagent or reagents in a dry form, for use as a support for artificially synthesised oligonucleotide sequences encoded with data. Preferably the matrix is fibrous (for example cellulose, or glass, fibres) formed into a support of sufficient strength to hold the oligonucleotide sequences. The stabilising reagents are preferably a combination of a weak base, and a chelating agent, optionally, uric acid or a urate salt, and optionally an anionic surfactant.

公开(公告)号：US20240018506A1

公开(公告)日：2024-01-18

申请号：US18216052

申请日：2023-06-29

申请人： Nutcracker Therapeutics, Inc.

发明人： Benjamin Eldridge ,  Ximiao Wen

IPC分类号： C12N15/10 ,  B01L3/00 ,  C12M1/36 ,  G01N21/01 ,  B01J19/00

CPC分类号： C12N15/101 ,  B01L3/502769 ,  C12M41/48 ,  G01N21/01 ,  B01J19/0046 ,  B01J19/0093 ,  B01L2400/0487 ,  G01N2021/0187 ,  B01J2219/00313 ,  B01J2219/00333 ,  B01J2219/00722 ,  B01J2219/00759

摘要： Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.

公开(公告)号：US11845054B2

公开(公告)日：2023-12-19

申请号：US17378133

申请日：2021-07-16

申请人： Gen9, Inc.

发明人： Joseph Jacobson ,  Li-Yun A. Kung ,  Andrew Kirk Wilson ,  Senthil Ramu ,  Daniel Schindler ,  Michael E. Hudson

IPC分类号： C12Q1/68 ,  B01J19/00 ,  C12N15/10 ,  C12N15/66 ,  C12P19/34

CPC分类号： B01J19/0046 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/66 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12P19/34 ,  C12N15/10 ,  C12Q2521/343 ,  C12Q2521/50 ,  C12N15/10 ,  C12Q2521/50 ,  C12Q2521/501 ,  C12N15/10 ,  C12N15/64

摘要： Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.

公开(公告)号：US11807907B2

公开(公告)日：2023-11-07

申请号：US17815806

申请日：2022-07-28

申请人： NEURIM PHARMACEUTICALS (1991) LTD.

发明人： Nava Zisapel ,  Moshe Laudon

IPC分类号： C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6827

CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16

摘要： Methods and products for identifying individuals who are likely to respond in a positive (benefit) or negative (harm) manner to a pharmacological drug treatment intended for treating or preventing a neuropsychiatric disorder, neurodegeneration, sleep-wake cycles such including and not limited to Alzheimer's disease, schizophrenia, autism and attention deficit disorders based on single nucleotide polymorphisms (SNP) chromosome 2, 2:107,510,000-107,540,000 locus (as disclosed in the Genome Reference Consortium Human genome build 37 (GRCh37)).

公开(公告)号：US20230338913A1

公开(公告)日：2023-10-26

申请号：US18319356

申请日：2023-05-17

申请人： TWIST BIOSCIENCE CORPORATION

发明人： William BANYAI ,  Bill James PECK ,  Andres FERNANDEZ ,  Siyuan CHEN ,  Pierre INDERMUHLE ,  Eugene P. MARSH

IPC分类号： B01J19/00 ,  C12N15/10

CPC分类号： B01J19/0046 ,  C12N15/1093 ,  B01J2219/00596 ,  B01J2219/00722 ,  B01J2219/00497 ,  B01J2219/00585 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00662 ,  B01L3/50857

摘要： Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

公开(公告)号：US11795503B2

公开(公告)日：2023-10-24

申请号：US16512276

申请日：2019-07-15

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： George A. Fry ,  Christina E. Inman ,  John Bridgham ,  Timothy Hunkapiller ,  Charles S. Vann

IPC分类号： C12Q1/6869 ,  G01N35/10 ,  B01L3/00 ,  G01N35/00 ,  G01N21/11 ,  B01F33/30 ,  B01F33/40 ,  B01J19/00 ,  C12Q1/6874 ,  G02B21/34 ,  G01N21/05

CPC分类号： C12Q1/6869 ,  B01F33/30 ,  B01F33/403 ,  B01J19/0046 ,  B01L3/50273 ,  B01L3/502715 ,  C12Q1/6874 ,  G01N21/11 ,  G01N35/00069 ,  G01N35/0099 ,  G01N35/1002 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00466 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00869 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0851 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  G01N21/05 ,  G01N35/1011 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

摘要： According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.