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公开(公告)号：US20240342678A1
公开(公告)日：2024-10-17
申请号：US18620775
申请日：2024-03-28
CPC分类号： B01J19/0046 , B01L3/5085 , C12N15/1013 , C12Q1/02 , C40B40/06 , G01N33/5005 , G01N33/54366 , B01J2219/00317 , B01J2219/005 , B01J2219/00648 , B01L2300/0829 , B01L2300/0851 , B01L2300/0858
摘要： The present invention provides methods, systems and kits for performing microwell analysis in sealed small volumes. The microwells of the invention are sealed through the pairing of microwell and bead geometry wherein the diameter of the beads is greater than the diameter of the bottom of the microwells. Loading the beads into the microwells seals samples within an analytical space between the bottom of a nested bead and the bottom of the subject microwell.
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公开(公告)号：US11795503B2
公开(公告)日：2023-10-24
申请号：US16512276
申请日：2019-07-15
IPC分类号： C12Q1/6869 , G01N35/10 , B01L3/00 , G01N35/00 , G01N21/11 , B01F33/30 , B01F33/40 , B01J19/00 , C12Q1/6874 , G02B21/34 , G01N21/05
CPC分类号： C12Q1/6869 , B01F33/30 , B01F33/403 , B01J19/0046 , B01L3/50273 , B01L3/502715 , C12Q1/6874 , G01N21/11 , G01N35/00069 , G01N35/0099 , G01N35/1002 , B01J19/0093 , B01J2219/00317 , B01J2219/00351 , B01J2219/00466 , B01J2219/00511 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00691 , B01J2219/00722 , B01J2219/00869 , B01L3/5025 , B01L3/502707 , B01L2200/025 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0851 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , G01N21/05 , G01N35/1011 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
摘要： According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
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公开(公告)号：US20180209067A1
公开(公告)日：2018-07-26
申请号：US15909447
申请日：2018-03-01
发明人： Richard H. Ebright
CPC分类号： C40B20/04 , B01J19/0046 , B01J2219/00459 , B01J2219/005 , B01J2219/00545 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , C40B30/04 , C40B40/06 , C40B40/10 , C40B50/04 , C40B50/16 , C40B70/00 , G01N21/29 , G01N21/64 , G01N21/6458 , G01N33/54313 , G01N33/582 , G01N33/6845 , G01N2021/1765
摘要： A method and apparatus for the physico-chemical encoding of a collection of beaded resin (“beads”) to determine the chemical identity of bead-anchored compounds by in-situ interrogation of individual beads. The present invention provides method and apparatus to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial compounds libraries as well as multiplexed diagnostic and environmental testing and other biochemical assays.
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公开(公告)号：US20170197193A1
公开(公告)日：2017-07-13
申请号：US15470577
申请日：2017-03-27
申请人： Illumina, Inc.
发明人： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
CPC分类号： C12Q1/6844 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00788 , C12Q1/686 , C40B50/18 , C12Q2527/125 , C12Q2535/122 , C12Q2565/543
摘要： A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
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公开(公告)号：US09650666B2
公开(公告)日：2017-05-16
申请号：US14812332
申请日：2015-07-29
申请人： Enzo Biochem, Inc.
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
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公开(公告)号：US09637777B2
公开(公告)日：2017-05-02
申请号：US14257294
申请日：2014-04-21
发明人： Michael Seul , Sukanta Banerjee , Jiacheng Yang , Tatiana Vener
CPC分类号： C12Q1/6832 , B01J2219/00459 , B01J2219/00576 , B01J2219/00608 , B01J2219/00648 , B01J2219/00722 , C12Q1/6809 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2565/501 , C12Q2565/507 , C12Q2533/101 , C12Q2565/514 , C12Q2565/519
摘要： Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.
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公开(公告)号：US09617585B2
公开(公告)日：2017-04-11
申请号：US15076063
申请日：2016-03-21
申请人： Enzo Biochem, Inc.
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
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公开(公告)号：US20170007977A1
公开(公告)日：2017-01-12
申请号：US15203582
申请日：2016-07-06
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/005 , B01J2219/00545 , B01J2219/00648 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , C12Q1/6806 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2563/155 , C12Q2565/607 , C12Q2565/629
摘要： A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.
摘要翻译： 制备离散聚合物网络阵列的方法包括将多个核酸聚合物网络与多个着色活化聚合物网络混合以形成分散体，将该分散体施加到孔阵列上，将核酸聚合物网络选择性地沉积到孔中 的孔阵列，并且冲洗孔阵列以选择性地去除多个着色活化聚合物网络。
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公开(公告)号：US20160362733A1
公开(公告)日：2016-12-15
申请号：US15188173
申请日：2016-06-21
申请人： ILLUMINA, INC.
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00317 , B01J2219/00459 , B01J2219/00466 , B01J2219/005 , B01J2219/00572 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00722 , B01L3/5085 , B82Y30/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C40B40/06 , C40B60/14 , C40B70/00 , G01N33/54313 , G06K9/6202 , Y10S977/924 , C12Q2563/155 , C12Q2563/179 , C12Q2563/107 , C12Q2527/137 , C12Q2521/313 , C12Q2537/143
摘要： The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.
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公开(公告)号：US09476854B2
公开(公告)日：2016-10-25
申请号：US15040815
申请日：2016-02-10
IPC分类号： G01N27/414 , B01J19/00
CPC分类号： G01N27/4473 , B01J19/0046 , B01J2219/00286 , B01J2219/00317 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00653 , B01J2219/00704 , B01J2219/00722 , C12Q1/6874 , G01N27/4145 , G01N27/4148 , G01N27/44743
摘要： An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.
摘要翻译： 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。
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