公开(公告)号：US12116698B1

公开(公告)日：2024-10-15

申请号：US17004403

申请日：2020-08-27

Applicant: The Trustees of Boston College

Inventor： Jianmin Gao

IPC: C40B40/02 ,  A61K38/00 ,  C07K14/47 ,  C12N7/00 ,  C12N15/10 ,  C40B30/00 ,  C40B30/04 ,  C40B40/00 ,  G01N33/50

CPC classification number: C40B40/02 ,  A61K38/00 ,  C07K14/4723 ,  C12N7/00 ,  C12N15/1037 ,  C40B30/04 ,  G01N33/5008 ,  C40B30/00 ,  C40B40/00

Abstract: Low molecular weight molecules able to penetrate cells and tissues and having high specificity and affinity for the surfaces of proteins. Methods of making same, pharmaceutical compositions comprising same, and methods of treating cancers, infectious diseases, and diseases and disorders associated with aberrant protein expression using same. A method for selecting a therapeutic peptide for binding to an isolated and/or purified protein of interest by screening a phage display library containing phage particles with phage display peptides which have at least one APBA modified cysteine residue. The APBA modified cysteine residues bind to surface lysine residues on the isolated and/or purified protein of interest by dynamic covalent conjugation to form iminoboronates.

公开(公告)号：US20240280569A1

公开(公告)日：2024-08-22

申请号：US18653506

申请日：2024-05-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  B01F33/3011 ,  B01F33/302 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

CPC classification number: G01N33/54366 ,  C12Q1/6874 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  B01F33/3011 ,  B01F33/3021 ,  C40B30/04

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20230408505A1

公开(公告)日：2023-12-21

申请号：US18354144

申请日：2023-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC classification number: G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20230280337A1

公开(公告)日：2023-09-07

申请号：US18305650

申请日：2023-04-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874

CPC classification number: G01N33/54366 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US11692191B2

公开(公告)日：2023-07-04

申请号：US16612880

申请日：2018-05-14

Applicant: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION

Inventor： Sung Hoon Kwon ,  Jun Hoi Kim ,  Seo Hee Chang ,  Ok Ju Kim

IPC: C12N15/10 ,  B01J19/00 ,  B01L3/00 ,  C40B40/00 ,  C40B60/12 ,  G01N33/543

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01L3/5027 ,  C40B40/00 ,  C40B60/12 ,  G01N33/54306 ,  G01N33/54386 ,  B01J2219/00644 ,  B01J2219/00743

Abstract: The present invention relates to a technique for genomic library screening and provides a method for separating, capturing, analyzing, and retrieving cells and cell products by using a microstructure that can be preferentially applied to the field of antibody engineering for the development of new therapeutic antibodies and can be extensively applied to multiple genetic/phenotypic analysis of various biochemical molecules, for example, in the field of protein engineering and metabolic engineering.

公开(公告)号：US20190085014A1

公开(公告)日：2019-03-21

申请号：US16149114

申请日：2018-10-01

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC: C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6876 ,  C07H19/10 ,  C12Q1/68 ,  C12Q1/6874 ,  C40B40/00

CPC classification number: C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20190031705A1

公开(公告)日：2019-01-31

申请号：US16150185

申请日：2018-10-02

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC: C07H19/14 ,  C12Q1/6869 ,  C07H19/10 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6872 ,  C12Q1/68 ,  C12Q1/686

CPC classification number: C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US09745619B2

公开(公告)日：2017-08-29

申请号：US14812388

申请日：2015-07-29

Applicant: Enzo Biochem, Inc.

Inventor： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Jack Coleman

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/00 ,  C12N15/10 ,  B01J19/00 ,  C40B40/00

CPC classification number: C40B40/06 ,  B01J19/0046 ,  B01J2219/00599 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07B2200/11 ,  C07H21/00 ,  C12N15/1006 ,  C12N15/1058 ,  C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/10 ,  G01N33/68 ,  C12Q2565/501 ,  C12Q2563/179 ,  C12Q2545/114 ,  C12Q2525/155 ,  C12Q2565/514 ,  C12Q2565/537

Abstract: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

公开(公告)号：US09725480B2

公开(公告)日：2017-08-08

申请号：US15167917

申请日：2016-05-27

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC: C07H19/14 ,  C12Q1/68 ,  C07H21/00 ,  C40B40/00

CPC classification number: C07H19/14 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C40B40/00 ,  C12Q2565/501 ,  C12Q2525/186

Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US09637778B2

公开(公告)日：2017-05-02

申请号：US14812254

申请日：2015-07-29

Applicant: Enzo Biochem, Inc.

Inventor： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Jack Coleman

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/00 ,  C12N15/10 ,  B01J19/00 ,  C40B40/00

CPC classification number: C40B40/06 ,  B01J19/0046 ,  B01J2219/00599 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07B2200/11 ,  C07H21/00 ,  C12N15/1006 ,  C12N15/1058 ,  C12Q1/68 ,  C12Q1/6809 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/10 ,  G01N33/68 ,  C12Q2565/501 ,  C12Q2563/179 ,  C12Q2545/114 ,  C12Q2525/155 ,  C12Q2565/514 ,  C12Q2565/537

Abstract: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.