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公开(公告)号：US20240175091A1
公开(公告)日：2024-05-30
申请号：US18544232
申请日：2023-12-18
IPC分类号： C12Q1/6886 , B01F23/41 , B01F25/433 , B01F33/3011 , B01F33/3031 , B01L3/00 , B01L7/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/686
CPC分类号： C12Q1/6886 , B01F23/41 , B01F25/4335 , B01F33/3011 , B01F33/3031 , B01L3/502784 , B01L7/52 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0883 , B01L2300/1822 , B01L2400/0415 , B01L2400/0487 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16
摘要： Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.
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公开(公告)号：US11891666B2
公开(公告)日：2024-02-06
申请号：US17223565
申请日：2021-04-06
IPC分类号： C12P19/34 , C12Q1/6886 , C12Q1/6806 , B01L3/00 , B01L7/00 , B01F23/41 , B01F25/433 , B01F33/3011 , B01F33/3031 , C12Q1/686 , C12Q1/6844
CPC分类号： C12Q1/6886 , B01F23/41 , B01F25/4335 , B01F33/3011 , B01F33/3031 , B01L3/502784 , B01L7/52 , C12Q1/686 , C12Q1/6806 , C12Q1/6844 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0883 , B01L2300/1822 , B01L2400/0415 , B01L2400/0487 , C12Q2600/118 , C12Q2600/158 , C12Q2600/16 , C12Q1/6806 , C12Q2563/159 , C12Q1/686 , C12Q2537/143 , C12Q2563/159 , C12Q2565/629
摘要： Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.
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公开(公告)号：US20230372935A1
公开(公告)日：2023-11-23
申请号：US18362530
申请日：2023-07-31
发明人： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz , Erin R. Chia , Billy W. Colston , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler
CPC分类号： B01L3/50273 , B01F23/41 , B01F33/813 , B01F33/3011 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/23
摘要： Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US11779923B2
公开(公告)日：2023-10-10
申请号：US16770135
申请日：2018-12-04
申请人： SAMPLIX APS
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/301 , B01F33/30 , B01F101/23
CPC分类号： B01L3/502784 , B01F23/4105 , B01F33/3011 , B01F33/3017 , B01L3/502707 , B01L3/502715 , B01F23/4143 , B01F23/4144 , B01F2101/23 , B01L2200/028 , B01L2200/06 , B01L2200/0673 , B01L2200/12 , B01L2200/141 , B01L2300/0681 , B01L2300/0867
摘要： The present invention relates to a microfluidic device, a method for manufacturing a microfluidic device, and a method for provision of double emulsion droplets using a microfluidic device. Furthermore, the present invention relates to an assembly configured to supply pressure to the microfluidic device for provision of double emulsion droplets. Furthermore, the present invention relates to a kit comprising a plurality of microfluidic devices and a plurality of fluids configured for use with the microfluidic device for provision of double emulsion droplets. The microfluidic device comprises a transfer conduit comprising a first transfer conduit part having a first affinity for water; and a collection conduit comprising a first collection conduit part having a second affinity for water being different from the first affinity for water. A well section and a microfluidic section of the microfluidic device are fixedly connected to each other.
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公开(公告)号：US12090480B2
公开(公告)日：2024-09-17
申请号：US18362530
申请日：2023-07-31
IPC分类号： B01L3/00 , B01F23/41 , B01F33/3011 , B01F33/81 , B01L9/00 , C12Q1/686 , B01F25/00 , B01F25/23 , B01F33/302 , G01N35/08
CPC分类号： B01L3/50273 , B01F23/41 , B01F33/3011 , B01F33/813 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/14 , B01F25/23 , B01F33/3021 , B01L3/502761 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , G01N35/08
摘要： Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.
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公开(公告)号：US11946929B2
公开(公告)日：2024-04-02
申请号：US18354144
申请日：2023-07-18
IPC分类号： G01N33/543 , B01F33/3011 , B01F33/302 , C12Q1/6874 , C40B30/04 , C40B40/00 , C40B40/04 , C40B70/00 , G01N33/532
CPC分类号： G01N33/54366 , C12Q1/6874 , C40B40/00 , C40B40/04 , C40B70/00 , G01N33/532 , B01F33/3011 , B01F33/3021 , C40B30/04
摘要： The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
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公开(公告)号：US20230398075A1
公开(公告)日：2023-12-14
申请号：US18208507
申请日：2023-06-12
发明人： Jung Yeon HAN , Donald Lad DEVOE
IPC分类号： A61K9/127 , B01J13/08 , B01F33/3011 , B01F25/10
CPC分类号： A61K9/1277 , B01J13/08 , B01F33/3011 , B01F25/10 , B01F2025/913
摘要： A first inlet flow can be directed along an axial direction in a hydrocyclonic flow cell. The first inlet flow can include first constituent molecules. At a same time, one or more second inlet flows can be directed along a circumferential direction of the hydrocyclonic flow cell. Each second inlet flow can include a buffer solution. The first inlet flow can be subjected to flow focusing by a surrounding primary vortex formed by the one or more second inlet flows, so as to generate a flow comprising a plurality of nanoparticles at an outlet of the hydrocyclonic flow cell. Each nanoparticle can be formed by a respective plurality of the first constituent molecules.
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公开(公告)号：US11794187B2
公开(公告)日：2023-10-24
申请号：US16359368
申请日：2019-03-20
CPC分类号： B01L3/502784 , B01F23/41 , B01F33/3011 , B01F33/813 , B01F35/10 , B01L3/502707 , B01L2200/027 , B01L2200/0673 , B01L2200/12 , B01L2300/0887 , B01L2300/0893
摘要： A microfluidic device contains a first layer having a plurality of channels, a second layer having a plurality of droplet makers, and a third layer having a plurality of through-holes connecting the plurality of channels to the plurality of droplet makers. The channels have a height of at least 4 times greater than the height of the droplet makers. The microfluidic device has at least 500 droplet makers in an area less than 10 cm2. The channels are formed by direct laser-micromachining and the droplet makers are formed by soft lithography molding.
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公开(公告)号：US11754499B2
公开(公告)日：2023-09-12
申请号：US16780508
申请日：2020-02-03
IPC分类号： B01L3/00 , G01N21/64 , B01J19/00 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/50 , C12Q1/6851 , B01F23/41 , B01F25/433 , B01F33/3011 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , G01N33/542 , G01N33/573 , G01N33/58 , B01L7/00
CPC分类号： G01N21/6445 , B01F23/41 , B01F25/433 , B01F25/4336 , B01F33/3011 , B01J19/0046 , B01L3/502761 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6851 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/5008 , G01N33/542 , G01N33/573 , G01N33/582 , B01J2219/0059 , B01J2219/0065 , B01J2219/0072 , B01J2219/0074 , B01J2219/00286 , B01J2219/00351 , B01J2219/00418 , B01J2219/00479 , B01J2219/00576 , B01J2219/00585 , B01J2219/00592 , B01J2219/00599 , B01J2219/00657 , B01J2219/00664 , B01J2219/00702 , B01J2219/00722 , B01J2219/00743 , B01L7/52 , B01L2200/027 , B01L2300/0636 , B01L2300/0645 , B01L2300/0654 , B01L2300/0681 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , G01N21/6428 , G01N2500/00 , C12Q1/6827 , C12Q2537/161 , C12Q2563/125 , C12Q2563/131 , C12Q2563/159 , C12Q1/6804 , C12Q2537/161 , C12Q2563/125 , C12Q2563/159 , C12Q1/6851 , C12Q2563/159 , C12Q2561/113 , C12Q2565/1015
摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.
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公开(公告)号：US20240253044A1
公开(公告)日：2024-08-01
申请号：US18376276
申请日：2023-10-03
申请人： 10x Genomics, Inc.
IPC分类号： B01L3/00 , B01F23/41 , B01F25/314 , B01F31/80 , B01F33/3011 , B01F33/302 , B01L3/02 , C12Q1/6806 , C12Q1/686 , C40B40/06 , G01N33/543 , G01N33/58
CPC分类号： B01L3/502784 , B01F23/41 , B01F23/4105 , B01F25/3141 , B01F25/31422 , B01F25/31423 , B01F31/84 , B01F33/3011 , B01F33/3021 , B01L3/0241 , B01L3/50273 , B01L3/502761 , C12Q1/6806 , G01N33/54313 , G01N33/58 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0851 , B01L2300/161 , B01L2400/086 , C12N2533/50 , C12Q1/686 , C12Q2563/185 , C40B40/06
摘要： Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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