公开(公告)号：US20170073750A1

公开(公告)日：2017-03-16

申请号：US15360369

申请日：2016-11-23

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark Oldham

IPC: C12Q1/68 ,  B01L3/00 ,  B03C1/28 ,  G01N27/414 ,  B03C5/00 ,  B03C5/02 ,  G01N33/543 ,  B01L7/00

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Abstract translation: 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US09150915B2

公开(公告)日：2015-10-06

申请号：US14596111

申请日：2015-01-13

Applicant: Genapsys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark Oldham

IPC: C12Q1/68 ,  G01N27/414 ,  H01L29/00

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

公开(公告)号：US20140045701A1

公开(公告)日：2014-02-13

申请号：US13824129

申请日：2011-10-04

Applicant: Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Parazi ,  Eric Nordman

Inventor： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Kosar Parazi ,  Eric Nordman

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Abstract translation: 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US08465922B2

公开(公告)日：2013-06-18

申请号：US13217112

申请日：2011-08-24

Applicant: John Eid ,  Stephen Turner

Inventor： John Eid ,  Stephen Turner

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  G01N21/00

CPC classification number: G01N33/58 ,  B01J2219/00693 ,  B01J2219/00702 ,  C12Q1/6837 ,  C40B60/10 ,  C40B60/12 ,  C12Q2537/143 ,  C12Q2565/102

Abstract: Methods and systems for monitoring reactions by observing signals deriving from those reactions, using signal processing that allows differentiation between signals that are otherwise optically overlapping by conventional detection methods. Centroid determination is used to identify signal sources that are presenting confounding overlapping signals due to their physical proximity, and/or to identify discrete signals from different reaction centers.

Abstract translation: 通过观察来自这些反应的信号来监测反应的方法和系统，使用允许通过常规检测方法光学重叠的信号之间的区别的信号处理。 质心确定用于识别由于其物理接近性而呈现混杂重叠信号的信号源，和/或识别来自不同反应中心的离散信号。

公开(公告)号：US08425844B2

公开(公告)日：2013-04-23

申请号：US13284868

申请日：2011-10-28

Applicant: Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

Inventor： Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC classification number: G01N33/5438 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00443 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/0075 ,  C40B60/04 ,  C40B60/10 ,  Y10T29/49117 ,  Y10T29/49155 ,  Y10T29/49826 ,  Y10T436/11

Abstract: Apparatus comprising surface site comprising substantially inorganic surface having chemical composition selected from group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within polypeptide bonding region and having selective bonding affinity for polypeptide; plurality of interlayers between which surface site is interposed; distal site end on surface site and distanced from interlayers, the surface being provided on distal site end; surface site and interlayers being interposed between first and second supports; first and second conductors provided on first and second supports and having respective first and second distal conductor ends positioned within polypeptide bonding region; conductors being capable of applying external voltage potential across polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising third conductor in electrical communication with surface site, the third conductor positioned for electrical communication with source of an external bias voltage. Techniques for making apparatus.

Abstract translation: 包括表面位置的装置包括具有选自金属，半导体，绝缘体及其混合物的化学组成的基本上无机表面，所述表面位于多肽结合区域内并且对多肽具有选择性结合亲和力; 介于其间的多个中间层; 表面部位的远端位置远离中间层，表面位于远端位置; 表面部位和夹层介于第一和第二支撑之间; 第一和第二导体，其设置在第一和第二支撑上，并且具有位于多肽接合区域内的相应的第一和第二远端导体末端; 导体能够跨越多肽接合区域施加外部电压电位。 装置，可选地包括这样的第一和第二支撑件和导体; 并且包括与表面部位电连通的第三导体，所述第三导体定位成与外部偏置电压源电连通。 制造设备的技术。

公开(公告)号：US20130096013A1

公开(公告)日：2013-04-18

申请号：US13632513

申请日：2012-10-01

Applicant: Hesaam Esfandyarpour ,  Mark Oldham ,  Eric Nordman

Inventor： Hesaam Esfandyarpour ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  H01L29/00

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: The present invention provides for methods and systems for Electronic DNA sequencing, single molecule DNA sequencing, and combinations of the above, providing low cost and convenient sequencing.

Abstract translation: 本发明提供电子DNA测序，单分子DNA测序及其组合的方法和系统，提供低成本和方便的测序。

公开(公告)号：US20120088308A1

公开(公告)日：2012-04-12

申请号：US13252169

申请日：2011-10-03

Applicant: Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

Inventor： Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

IPC: G01N33/68

CPC classification number: G01N33/5438 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00443 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/0075 ,  C40B60/04 ,  C40B60/10 ,  Y10T29/49117 ,  Y10T29/49155 ,  Y10T29/49826 ,  Y10T436/11

Abstract: Method that includes providing plurality of test sites each having first and second layers respectively including inorganic first and second surface sites forming parts of interior of a well, the surface sites having positions and thicknesses being configured for locating thereon portion of unidentified amino acid-containing molecules; exposing each of a first plurality of the test sites to a fluid containing a different one of plurality of pre-identified amino acid-containing molecules and determining bonding signatures onto each of first plurality of test sites; exposing each of second plurality of test sites to another fluid containing unidentified amino acid-containing molecule and determining bonding signatures onto second plurality of test sites; and comparing bonding signatures to determine or exclude identity of unidentified amino acid-containing molecule.

Abstract translation: 包括提供多个测试位置的方法，每个测试位置各自具有分别包括形成井的内部的部分的无机第一和第二表面位置的第一和第二层，所述表面位置具有配置用于定位其中不明氨基酸分子的部分 ; 将第一多个测试位点中的每一个暴露于含有多个预先识别的含氨基酸分子中的不同一个的流体，并将结合签名确定到第一多个测试位点中的每一个上; 将第二多个测试位点中的每一个暴露于含有不确定含氨基酸的分子的另一种流体，并将结合签名确定到第二多个测试位点上; 并比较结合特征以确定或排除未鉴定的含氨基酸分子的同一性。

公开(公告)号：US20100069252A1

公开(公告)日：2010-03-18

申请号：US12513031

申请日：2007-10-30

Applicant: Bakshy Akshaykirit Chibber

Inventor： Bakshy Akshaykirit Chibber

IPC: G01N33/68 ,  C40B60/10 ,  C40B30/00

CPC classification number: C40B60/10 ,  C07K1/128 ,  C40B20/08

Abstract: A method and system for sequencing polypeptides utilizing acid and base labile xanthates.

Abstract translation: 使用酸和碱不稳定黄原酸盐测序多肽的方法和系统。

公开(公告)号：US20090270264A1

公开(公告)日：2009-10-29

申请号：US12421124

申请日：2009-04-09

Applicant: Thomas L. Overson

Inventor： Thomas L. Overson

IPC: C40B20/06 ,  C40B60/10

CPC classification number: G16B20/00 ,  C40B20/06 ,  C40B60/10

Abstract: A total forensic DNA casework management system and method for the deconvolution of mixed DNA samples using a novel, 3-rule algorithm to determine the proportional allele sharing of the sample's contributors. The process is fully document, can assess and process DNA anomalies and artifacts, and transforms raw STR data to produce final DNA profile types, peak height ratios, proportions, fitting criteria and associated graphs.

Abstract translation: 一个全面的法医DNA个案工作管理系统和混合DNA样本的去卷积方法，采用新颖的3规则算法来确定样本贡献者的比例等位基因共享。 该过程是完整的文件，可以评估和处理DNA异常和伪像，并转换原始的STR数据，以产生最终的DNA分布类型，峰高比，比例，拟合标准和相关图。

公开(公告)号：US20090163384A1

公开(公告)日：2009-06-25

申请号：US12004628

申请日：2007-12-22

Applicant: Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

Inventor： Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

IPC: C40B60/10

CPC classification number: G01N33/5438 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00443 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/0075 ,  C40B60/04 ,  C40B60/10 ,  Y10T29/49117 ,  Y10T29/49155 ,  Y10T29/49826 ,  Y10T436/11

Abstract: Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus.

Abstract translation: 包括表面位置的装置包括具有选自金属，半导体，绝缘体及其混合物的化学组成的基本无机表面，所述表面位于多肽结合区域内并且对多肽具有选择性结合亲和力; 多个中间层，其间插入表面部位; 位于表面部位的远侧位置端部并且远离中间层，所述表面设置在远端位置端部; 表面部位和夹层介于第一和第二支撑之间; 第一和第二导体，其设置在第一和第二支撑件上，并且具有位于多肽接合区域内的相应的第一和第二远端导体末端; 导体能够跨越多肽接合区域施加外部电压电位。 装置，可选地包括这样的第一和第二支撑件和导体; 并且包括与表面部位电连通的第三导体，所述第三导体定位成与外部偏置电压源电连通。 制造设备的技术。