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    ARRAYS AND METHODS FOR GUIDED CELL PATTERNING
    4.
    发明申请
    ARRAYS AND METHODS FOR GUIDED CELL PATTERNING 审中-公开
    用于指导细胞图案的阵列和方法

    公开(公告)号：US20140018260A1

    公开(公告)日：2014-01-16

    申请号：US13846681

    申请日：2013-03-18

    申请人： University of Washington

    发明人： Miqin Zhang ,  Mandana Veiseh

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5008 ,  C40B20/02 ,  C40B40/02 ,  C40B60/04 ,  G01N33/5005 ,  H01L21/02164

    摘要： Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion.

    摘要翻译： 公开了用于单细胞图案化的引导细胞图案阵列。 阵列包括在惰性表面上单独分离的多个细胞粘附位点。 每个细胞粘附位点具有对细胞表面受体具有亲和性的一种或多种细胞粘附肽。 惰性表面耐细胞粘附。

    MULTI-DIMENSIONAL SELECTION OF PROTEIN MUTANTS USING HIGH THROUGHPUT SEQUENCE ANALYSIS
    6.
    发明申请
    MULTI-DIMENSIONAL SELECTION OF PROTEIN MUTANTS USING HIGH THROUGHPUT SEQUENCE ANALYSIS 审中-公开
    使用高通量序列分析进行多维选择蛋白质突变体

    公开(公告)号：US20120258866A1

    公开(公告)日：2012-10-11

    申请号：US13424383

    申请日：2012-03-20

    申请人： Robert DuBridge

    发明人： Robert DuBridge

    IPC分类号： C40B20/00

    CPC分类号： C07K16/005 ,  C07K16/22 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/92 ,  C12N15/1034 ,  C12N15/1037 ,  C40B60/04

    摘要： The invention is directed to methods for simultaneously improving a plurality of characteristics of a protein binding compound. In accordance with one aspect of the invention, a focused library of nucleic acid-encoded variants is produced and separately exposed to a plurality of reaction conditions each designed to segregate the library variants according to a different characteristic of interest, such as affinity, stability, cross-reactivity, or the like. In various embodiments, such reactions may be conducted pair-wise to simultaneously obtain improvements in two characteristics or they may be conducted three-at-a-time to simultaneously obtain improvements in three characteristics. In each case, nucleotide sequences encoding library variants segregated into improved subsets are determined, after which sequences occurring in two or more subsets are identified to obtain library variants with two or more improved characteristics.

    摘要翻译： 本发明涉及同时改善蛋白质结合化合物的多种特征的方法。 根据本发明的一个方面，产生核酸编码变体的集中文库，并分别暴露于多个反应条件，每个反应条件被设计为根据不同的目标特征分离文库变体，例如亲和力，稳定性， 交叉反应性等。 在各种实施方案中，这些反应可以成对进行以同时获得两个特征的改进，或者它们可以一次三次进行，以同时获得三个特征的改进。 在每种情况下，确定分离成改进子集的编码文库变体的核苷酸序列，之后鉴定出两个或多个子集中出现的序列以获得具有两个或更多个改进特征的文库变体。

    Detection Apparatus for Biological Materials and Methods of Making and Using the Same
    7.
    发明申请
    Detection Apparatus for Biological Materials and Methods of Making and Using the Same 有权
    生物材料检测装置及其使用方法

    公开(公告)号：US20110123401A1

    公开(公告)日：2011-05-26

    申请号：US13020446

    申请日：2011-02-03

    申请人： Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

    发明人： Robert L. Willett ,  Kirk W. Baldwin ,  Loren N. Pfeiffer

    IPC分类号： G01N30/00 ,  B23P17/04

    CPC分类号： G01N33/5438 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00443 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/0075 ,  C40B60/04 ,  C40B60/10 ,  Y10T29/49117 ,  Y10T29/49155 ,  Y10T29/49826 ,  Y10T436/11

    摘要： Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus.

    摘要翻译： 包括表面位置的装置包括具有选自金属，半导体，绝缘体及其混合物的化学组成的基本无机表面，所述表面位于多肽结合区域内并且对多肽具有选择性结合亲和力; 多个中间层，其间插入表面部位; 位于表面部位的远侧位置端部并且远离中间层，所述表面设置在远端位置端部; 表面部位和夹层介于第一和第二支撑之间; 第一和第二导体，其设置在第一和第二支撑件上，并且具有位于多肽接合区域内的相应的第一和第二远端导体末端; 导体能够跨越多肽接合区域施加外部电压电位。 装置，可选地包括这样的第一和第二支撑件和导体; 并且包括与表面部位电连通的第三导体，所述第三导体定位成与外部偏置电压源电连通。 制造设备的技术。