公开(公告)号：US12054708B2

公开(公告)日：2024-08-06

申请号：US16345895

申请日：2017-10-30

Applicant: Universität Zürich

Inventor： Markus Seeger ,  Pascal Egloff ,  Iwan Zimmermann

IPC: C12N15/10 ,  C07K16/12 ,  G01N33/68

CPC classification number: C12N15/1065 ,  C07K16/12 ,  C12N15/1034 ,  C12N15/1093 ,  G01N33/6845 ,  G01N33/6848 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/41 ,  C07K2319/43 ,  C12Q2563/119 ,  C12Q2563/185 ,  C12N15/1034 ,  C12Q2563/119 ,  C12Q2563/185 ,  C12N15/1065 ,  C12Q2563/119 ,  C12Q2563/185

Abstract: The invention relates to a method for identifying and quantifying a polypeptide from a library of polypeptides. The method comprises the steps of: 1—providing a polypeptide library and a detection tag library, 2—generating a nested library comprising the polypeptides and the detection tags, 3—sequencing the nested library, 4—selecting a member of the nested library in one or several selection steps that are independent of a physical genotype-phenotype linkage, 5—isolating the detection tag from the selected polypeptide, 6—identifying and quantifying the detection tag by mass spectrometry, 7—obtaining the sequence of the selected polypeptide. The invention also relates to a collection of polypeptides, a collection of detection tags, and a collection of plasmid vectors.

公开(公告)号：US20190210018A1

公开(公告)日：2019-07-11

申请号：US16139831

申请日：2018-09-24

Applicant: Plexium, Inc.

Inventor： Kandaswamy VIJAYAN ,  Andrew Boyd MACCONNELL ,  Joseph Franklin Rokicki ,  Michael VAN NGUYEN

IPC: B01L3/00 ,  C12N15/10 ,  C40B30/04

CPC classification number: B01L3/50853 ,  B01L2300/0829 ,  C12N15/1034 ,  C12N15/1065 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2600/16 ,  C40B30/04 ,  C40B30/06 ,  C40B50/04 ,  C40B50/14 ,  C40B50/16 ,  C12Q2563/179

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

公开(公告)号：US20180230534A1

公开(公告)日：2018-08-16

申请号：US15951109

申请日：2018-04-11

Applicant: Single Cell Technology, Inc.

Inventor： Chun-Nan Chen ,  James O. Bowlby, JR.

IPC: C12Q1/6874 ,  C12N15/10 ,  C40B30/06 ,  C40B30/04

CPC classification number: C12Q1/6874 ,  C12N15/1034 ,  C12N15/1086 ,  C40B30/04 ,  C40B30/06

Abstract: This invention generally relates to methods, devices and kits for screening a plurality of single secreting cells for functional activity of the secreted molecules by measuring the amount of reporter gene mRNA produced in one or more reporter cells in response to the secreted molecules.

公开(公告)号：US20180141979A1

公开(公告)日：2018-05-24

申请号：US15724420

申请日：2017-10-04

Applicant: Ulla Bonas ,  Jens Boch ,  Sebastian Schornack ,  Thomas Lahaye

Inventor： Ulla Bonas ,  Jens Boch ,  Sebastian Schornack ,  Thomas Lahaye

IPC: C07K14/195 ,  C07K14/00 ,  C12N5/071 ,  C12N5/04 ,  C12N1/20 ,  C12N15/10 ,  C07H21/04 ,  C12N15/82 ,  G01N33/53 ,  C12Q1/6816

CPC classification number: C07K14/195 ,  C07H21/04 ,  C07K14/001 ,  C07K2319/09 ,  C07K2319/60 ,  C07K2319/80 ,  C12N1/20 ,  C12N5/04 ,  C12N5/0602 ,  C12N15/1034 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/825 ,  C12N15/8279 ,  C12N2501/998 ,  C12Q1/6816 ,  C12Q2563/119 ,  G01N33/5308 ,  C12Q2565/113

Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.

公开(公告)号：US09944983B2

公开(公告)日：2018-04-17

申请号：US14364520

申请日：2012-12-12

Applicant: Single Cell Technology, Inc.

Inventor： Chun-Nan Chen ,  James O. Bowlby, Jr.

IPC: C12N15/10 ,  C12Q1/68 ,  C40B30/04 ,  C40B30/06

CPC classification number: C12Q1/6874 ,  C12N15/1034 ,  C12N15/1086 ,  C40B30/04 ,  C40B30/06

Abstract: This invention generally relates to methods, devices and kits for screening a plurality of single secreting cells for functional activity of the secreted molecules by measuring the amount of reporter gene mRNA produced in one or more reporter cells in response to the secreted molecules.

公开(公告)号：US20180086814A1

公开(公告)日：2018-03-29

申请号：US15716302

申请日：2017-09-26

Applicant: Heptares Therapeutics Limited

Inventor： Richard Henderson ,  Christopher Gordon Tate ,  Francesca Magnani ,  Maria Josefa Serrano-Vega ,  Yoko Shibata ,  Antony Johannes Warne ,  Malcolm Peter Weir

IPC: C07K14/72 ,  C12N15/10 ,  G01N33/566

CPC classification number: C07K14/723 ,  C12N15/1034 ,  G01N33/566 ,  G01N2333/726

Abstract: The invention relates to mutant G-protein coupled receptors with increased conformational stability, and methods of use thereof. In some aspects, polynucleotides encoding the mutant G-protein coupled receptors are provided. In some aspects, host cells comprising the polynucleotides are provided. In some aspects, the invention relates to crystallized forms of the mutant G-protein coupled receptors, and methods of preparing the same.

公开(公告)号：US20180073023A1

公开(公告)日：2018-03-15

申请号：US15690699

申请日：2017-08-30

Applicant: HITACHI, LTD.

Inventor： Hiroko MATSUNAGA ,  Maiko TANABE

IPC: C12N15/113 ,  A61K31/70 ,  C12Q1/68

CPC classification number: C12N15/113 ,  A61K31/70 ,  A61K38/00 ,  C12N15/1034 ,  C12N15/1065 ,  C12N2310/152 ,  C12N2310/53 ,  C12Q1/6806 ,  C12Q1/6813 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2531/113 ,  C12Q2531/125 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: An object of the present invention is to provide a circular single-stranded nucleic acid, and a method for preparing the same and a method for using the same. A circular single-stranded nucleic acid according to one embodiment of the present invention is a circular single-stranded nucleic acid for determining a target base on a genomic DNA, and includes a first single-stranded nucleic acid which has the target base or a complementary base thereto and is a part of one of the strands of the genomic DNA, and a second single-stranded nucleic acid which has an index sequence to serve as an index of a cell, from which the genomic DNA is derived, or a complementary sequence thereto.

公开(公告)号：US20180023075A1

公开(公告)日：2018-01-25

申请号：US15605586

申请日：2017-05-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Robert POTTER ,  Namritha RAVINDER ,  Shantanu KUMAR

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1082 ,  A01K2217/07 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/1034 ,  C12N15/113 ,  C12N15/907 ,  C12N2310/152 ,  C12N2310/153 ,  C12N2310/20 ,  C12N2310/533 ,  C12Q2600/156

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

公开(公告)号：US09809628B2

公开(公告)日：2017-11-07

申请号：US15222498

申请日：2016-07-28

Applicant: Ulla Bonas ,  Jens Boch ,  Sebastian Schornack ,  Thomas Lahaye

Inventor： Ulla Bonas ,  Jens Boch ,  Sebastian Schornack ,  Thomas Lahaye

IPC: C07K14/195 ,  C12N15/10 ,  C12N15/82 ,  C12Q1/68 ,  G01N33/53 ,  C12N1/20 ,  C12N5/071 ,  C07H21/04 ,  C07K14/00 ,  C12N5/04

CPC classification number: C07K14/195 ,  C07H21/04 ,  C07K14/001 ,  C07K2319/09 ,  C07K2319/60 ,  C07K2319/80 ,  C12N1/20 ,  C12N5/04 ,  C12N5/0602 ,  C12N15/1034 ,  C12N15/8216 ,  C12N15/8217 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/825 ,  C12N15/8279 ,  C12N2501/998 ,  C12Q1/6816 ,  C12Q2563/119 ,  G01N33/5308 ,  C12Q2565/113

Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.

公开(公告)号：US20170218033A1

公开(公告)日：2017-08-03

申请号：US15500803

申请日：2015-07-31

Applicant: Helge Zieler ,  Sabrina German ,  Animesh Ray ,  Biranchi Narayan Patra ,  Primordial Genetics Inc.

Inventor： Helge ZIELER ,  Sabrina GERMAN ,  Animesh RAY ,  Biranchi Narayan PATRA

IPC: C07K14/395 ,  C12N15/81 ,  C12N15/70 ,  C12N15/10

CPC classification number: C07K14/395 ,  C07K2319/00 ,  C12N15/1034 ,  C12N15/1093 ,  C12N15/70 ,  C12N15/81

Abstract: The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to obtain desirable phenotypic changes that modulate tolerance to stress, thus creating novel characteristics of the transformed organism.