公开(公告)号：US12123888B2

公开(公告)日：2024-10-22

申请号：US16878549

申请日：2020-05-19

Applicant: Isolation Bio Inc.

Inventor： Peter Christey ,  Alexander Hallock

IPC: G01N35/10 ,  B01L3/00 ,  B01L3/02 ,  C12M1/04 ,  C12M1/26 ,  C12M1/32 ,  C12Q1/6853 ,  G01N35/00 ,  G06T7/73

CPC classification number: G01N35/1081 ,  B01L3/5085 ,  C12M23/12 ,  C12M23/24 ,  C12M33/04 ,  C12Q1/6853 ,  G01N35/00029 ,  G01N35/00693 ,  G01N35/1004 ,  G06T7/74 ,  B01L3/0244 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/141 ,  B01L2300/021 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/165 ,  G01N2035/00148 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30204 ,  G06T2207/30232 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2547/101 ,  C12Q2563/159 ,  C12Q2563/179

Abstract: A picking instrument for picking biological samples, such as microbial samples, includes a picking pin having a distal tip and three degrees-of-freedom and configured to move in x, y, and z directions. The picking instrument also includes a loading platform comprising a first area and a second area, the first area configured to accommodate and secure a microfabricated chip including a plurality of microwells. The picking pin is programmatically controlled to pick a sample contained in one or more selected microwells of the microfabricated chip and then transfer the sample to a predetermined location at the destination sample holder. Methods of operating the picking instrument, including calibrating the coordinates of the selected microwell(s) relative to a location of the picking pin, are also provided.

公开(公告)号：US12071651B2

公开(公告)日：2024-08-27

申请号：US16533444

申请日：2019-08-06

Applicant: BillionToOne, Inc.

Inventor： David Tsao ,  Patrick Ye ,  Sukrit Silas ,  Oguzhan Atay

IPC: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6876 ,  G16B40/10

CPC classification number: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6876 ,  G16B40/10 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2537/143 ,  C12Q2545/114 ,  C12Q2600/166

Abstract: Embodiments of a method for accurate determination of biological target abundance can include generating a first set of molecules associated with a target sequence, where the first set of molecules includes a first set of dilution tags associated with a relative concentration profile; generating a second set of molecules including a second set of dilution tags associated with the first set of dilution tags; generating a dilution tagged mixture; amplifying the subsets of dilution tagged genetic targets using the second set of molecules; generating a modified dilution tagged mixture from the amplified subsets; determining, for the biological sample, a count of the distinct molecules including the target sequence; and/or determining, for the biological sample, an assessment of relative concentrations distinct species, such as over a vast dynamic range.

公开(公告)号：US12071615B2

公开(公告)日：2024-08-27

申请号：US17485599

申请日：2021-09-27

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/70 ,  Y02A50/30 ,  C12Q1/6806 ,  C12Q2525/161 ,  C12Q2527/125 ,  C12Q2531/143 ,  C12Q2565/501 ,  C12Q1/70 ,  C12Q2525/161 ,  C12Q2527/125 ,  C12Q2531/143 ,  C12Q2565/501

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US12065693B2

公开(公告)日：2024-08-20

申请号：US17061552

申请日：2020-10-01

Applicant: GEN-PROBE INCORPORATED

Inventor： Michael M. Becker ,  Kristin W. Livezey ,  Wai-Chung Lam

IPC: C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q1/6832 ,  C12Q2565/107 ,  C12Q2563/179 ,  C12Q2527/101 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q1/6834 ,  C12Q2565/107 ,  C12Q2563/179 ,  C12Q2527/101 ,  C12Q1/6834 ,  C12Q2537/1373 ,  C12Q2525/301 ,  C12Q2525/161

Abstract: The present invention provides compositions, kits and methods for the selective hybridization and capture of a specific target nucleic acid. The specific target nucleic acid may be present in a heterogeneous mixture of nucleic acids. Selective hybridization and capture provides a target nucleic acid that is substantially free of non-target and/or contaminating nucleic acids. Target nucleic acids that have been selectively hybridized and captured using the current invention are then used in subsequent analysis, wherein the presence of non-target and/or contaminating nucleic acids that interfere with said subsequent analysis have been substantially reduced or eliminated, thereby providing improved analysis results. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in subsequent analysis, or present in the environment in which an assay is performed, are free of bacterial or other contaminating nucleic acids.

公开(公告)号：US20240240233A1

公开(公告)日：2024-07-18

申请号：US18389901

申请日：2023-12-20

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Georg SEELIG ,  Alexander B. ROSENBERG ,  Charles ROCO

IPC: C12Q1/6806

CPC classification number: C12Q1/6806 ,  C12Q2521/107 ,  C12Q2525/161 ,  C12Q2543/101 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/514

Abstract: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.

公开(公告)号：US20240240232A1

公开(公告)日：2024-07-18

申请号：US18389890

申请日：2023-12-20

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Georg SEELIG ,  Alexander B. ROSENBERG ,  Charles ROCO

IPC: C12Q1/6806

CPC classification number: C12Q1/6806 ,  C12Q2521/107 ,  C12Q2525/161 ,  C12Q2543/101 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/514

Abstract: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.

公开(公告)号：US11952621B2

公开(公告)日：2024-04-09

申请号：US17306761

申请日：2021-05-03

Applicant: Cepheid

Inventor： Russell Higuchi

IPC: C12Q1/68 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2531/119 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2531/119

Abstract: Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR.

公开(公告)号：US20240076719A9

公开(公告)日：2024-03-07

申请号：US18160816

申请日：2023-01-27

Applicant: Oxford Nanopore Technologies PLC

Inventor： Nicholas Antony Smith ,  Daniel John Turner ,  Daniel George Fordham ,  James White

IPC: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6876

CPC classification number: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q2525/101 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising:



(i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein:

(a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and
(b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides;


(ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore;
(iii) measuring current blockades having a duration within a defined window, wherein:

(a) the one or more non-natural nucleotides present in the hybridisation region of the probe increase or decrease the duration of the current blockade due to the probe hybridised to its target polynucleotide such that the proportion of current blockades that occur within the window due to the interaction of the hybridised probes with the pore is increased compared to when the corresponding one or more natural nucleotides are present in the hybridisation region; and
(b) each hybridised probe gives rise to a current blockade indicative of that probe; and


(iv) correlating the measured current blockades with the probes, thereby determining the presence, absence or amount of the two or more target polynucleotides in the sample.

公开(公告)号：US11905551B2

公开(公告)日：2024-02-20

申请号：US17508674

申请日：2021-10-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti ,  George Karlin-Neumann

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6832 ,  C12Q1/682

CPC classification number: C12Q1/6806 ,  C12Q1/682 ,  C12Q1/686 ,  C12Q1/6811 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/119 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/537

Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.

公开(公告)号：US11795494B2

公开(公告)日：2023-10-24

申请号：US17166992

申请日：2021-02-03

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/6806 ,  C12Q1/686 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6806 ,  B01L3/502738 ,  C12Q1/686 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/087 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.