公开(公告)号：US12221720B2

公开(公告)日：2025-02-11

申请号：US16763681

申请日：2018-11-13

Applicant: The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

Inventor： Naomi Habib ,  Aviv Regev ,  Eugene Drokhlyansky ,  Anindita Basu ,  Inbal Avraham-Davidi ,  Orit Rozenblatt-Rosen ,  David A. Weitz

IPC: C40B50/06 ,  A61K35/30 ,  C12N5/0793 ,  C12N5/0797 ,  C12Q1/6806 ,  C12Q1/6881 ,  C40B30/06 ,  C12N5/00

Abstract: Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

公开(公告)号：US12054852B2

公开(公告)日：2024-08-06

申请号：US15665226

申请日：2017-07-31

Applicant: The Regents of the University of California

Inventor： Vivek K. Mutalik ,  Adam Deutschbauer ,  Adam P. Arkin ,  Pavel Novichkov

IPC: C40B30/06 ,  C12N15/10

CPC classification number: C40B30/06 ,  C12N15/1065

Abstract: Disclosed herein are barcoded expression libraries comprising a plurality of expression vectors, wherein each expression vector comprises a nucleic acid fragment flanked by a first barcode and a second barcode. Further disclosed herein are methods of making the barcoded expression libraries and methods of conducting functional analysis using the barcoded expression libraries.

公开(公告)号：US20240026456A1

公开(公告)日：2024-01-25

申请号：US18458199

申请日：2023-08-30

Applicant: CORNELL UNIVERSITY

Inventor： Iwijn DE VLAMINCK ,  John Richard LEE ,  Philip Smith BURNHAM ,  Alexandre Pellan CHENG ,  Manikkam SUTHANTHRIAN ,  Darshana M. DADHANIA

IPC: C12Q1/6883 ,  C12Q1/689 ,  C12Q1/70 ,  C40B30/06

CPC classification number: C12Q1/6883 ,  C12Q1/689 ,  C12Q1/701 ,  C40B30/06 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: The present disclosure is directed to methods of detecting cell-free DNA (cfDNA) in biological samples and using it to quantify organ damage and identify pathogens. In some aspects, the biological samples are from patients who have undergone solid-organ transplantation. The disclosure is also directed to methods of detecting and analyzing methylation patterns in cell-free DNA from organ transplant patients to identify the presence of pathogens as well as quantify contributing tissue proportions as a measurement of the host response.

公开(公告)号：US20230392184A1

公开(公告)日：2023-12-07

申请号：US18446048

申请日：2023-08-08

Applicant: PHAST CORP.

Inventor： Kwangmin SON ,  Roman STOCKER

IPC: C12Q1/04 ,  G02B21/00 ,  G02B21/36 ,  C40B30/06 ,  C40B40/02 ,  G01N33/94 ,  G16C10/00

CPC classification number: C12Q1/04 ,  G02B21/0084 ,  G02B21/365 ,  C40B30/06 ,  C40B40/02 ,  G01N33/9446 ,  G16C10/00

Abstract: Among other things, motility of at least one individual microorganism or a change in motility of at least one individual microorganism or both is or are characterized. The characterized motility or change in motility is used to detect the presence or count of the at least one individual microorganism, or determine the identity of a species or strain of the at least one individual microorganism, or determine a susceptibility of the at least one individual microorganism to one or more antibiotics or other antimicrobials.

公开(公告)号：US11795579B2

公开(公告)日：2023-10-24

申请号：US16771128

申请日：2018-12-10

Applicant: Abalone Bio, Inc.

Inventor： Richard Yu ,  Carlos Gustavo Pesce ,  Rodrigo Baltanas ,  Brett Robison

IPC: C40B40/02 ,  C07K16/28 ,  C12N15/10 ,  C40B30/04 ,  C40B30/06 ,  C07K16/00

CPC classification number: C40B40/02 ,  C07K16/00 ,  C07K16/28 ,  C12N15/1037 ,  C40B30/04 ,  C40B30/06

Abstract: Compositions and methods for displaying antibodies in the periplasmic space of yeast cells are disclosed. In particular, antibodies are linked to a cell membrane-spanning transmembrane domain, a cell-membrane associated protein domain that is on the external face of the yeast cell membrane, a protein that binds to the inner face of the yeast cell wall, or a periplasmic protein in order to display the antibodies in the yeast periplasmic space. In addition, a target protein of interest can be coexpressed in yeast such that it is localized to the plasma membrane or periplasmic space and accessible to binding by displayed antibodies. The disclosure further relates to high-throughput screening of antibody libraries using yeast cell periplasmic display.

公开(公告)号：US11686014B2

公开(公告)日：2023-06-27

申请号：US16636523

申请日：2018-08-06

Applicant: CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

Inventor： Kwang-Hyun Baek ,  So-Ra Kim ,  Seul-Ki Kwon ,  Soo-Yeon Kim ,  Da-Hye Lee

IPC: C40B30/06 ,  C12N9/48 ,  C12N15/10 ,  C12N15/81 ,  C12Q1/37 ,  C40B40/02 ,  C40B40/08

CPC classification number: C40B30/06 ,  C12N9/48 ,  C12N15/1055 ,  C12N15/81 ,  C12Q1/37 ,  C40B40/02 ,  C40B40/08

Abstract: The present invention provides a vector library for yeast two-hybrid screening of a deubiquitinating enzyme that binds to a target protein and a method for identifying a deubiquitinating enzyme binding to a target protein using the same. Also, the present invention provides a method for screening an agent having anti-cancer activity targeting the deubiquitinating enzyme USP1, USP7, USP12, or USP49 identified by said identifying method.

公开(公告)号：US11577249B2

公开(公告)日：2023-02-14

申请号：US17236283

申请日：2021-04-21

Applicant: Plexium, Inc.

Inventor： Kandaswamy Vijayan ,  Andrew Boyd MacConnell ,  Joseph Franklin Rokicki

IPC: C40B30/06 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/04 ,  C40B50/14 ,  C12Q1/6876 ,  C40B50/16 ,  C40B30/04

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

公开(公告)号：US11359195B2

公开(公告)日：2022-06-14

申请号：US15655784

申请日：2017-07-20

Applicant: DISCUVA LIMITED

Inventor： David Hugh Williams ,  Arthur Keith Turner

IPC: C12N15/10 ,  C40B30/06 ,  G16B20/00 ,  G16B20/20 ,  G16B20/50

Abstract: Disclosed are methods related to identifying an essential gene which serves as an antibiotic target in a bacterium.

公开(公告)号：US10941434B2

公开(公告)日：2021-03-09

申请号：US16104319

申请日：2018-08-17

Applicant: The Trustees of Princeton University

Inventor： Mohammad R. Seyedsayamdost

IPC: C40B30/06 ,  C12Q1/02 ,  C12N15/52 ,  C12P17/06

Abstract: The majority of clinically used antibiotics and anticancer agents are derived from bacterial small molecules. These molecules are produced by dedicated biosynthetic gene clusters, sets of genes that are responsible for the step-wise generation of the target small molecule. Recent investigations have indicated, to the surprise of many experts, that the majority of these biosynthetic genes are inactive or ‘silent’ for unknown reasons. Thus under typical bacterial culturing conditions, these genes are not expressed and consequently the bioactive small molecule products are not synthesized. Disclosed is a method for high throughput screening of elicitors of cryptic metabolites, a method for producing cryptic metabolites, and a new family of cryptic metabolites, the acybolins, as well as their complete structural elucidation.

公开(公告)号：US20200324287A1

公开(公告)日：2020-10-15

申请号：US16870809

申请日：2020-05-08

Applicant: Plexium, Inc.

Inventor： Kandaswamy VIJAYAN ,  Andrew Boyd MACCONNELL ,  Joseph Franklin ROKICKI ,  Michael VAN NGUYEN

IPC: B01L3/00 ,  C12N15/10 ,  C12Q1/6869 ,  C40B50/04 ,  C40B50/14 ,  C12Q1/6876 ,  C40B30/06 ,  C40B50/16 ,  C40B30/04

Abstract: This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.