公开(公告)号：US12221720B2

公开(公告)日：2025-02-11

申请号：US16763681

申请日：2018-11-13

Applicant: The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

Inventor： Naomi Habib ,  Aviv Regev ,  Eugene Drokhlyansky ,  Anindita Basu ,  Inbal Avraham-Davidi ,  Orit Rozenblatt-Rosen ,  David A. Weitz

IPC: C40B50/06 ,  A61K35/30 ,  C12N5/0793 ,  C12N5/0797 ,  C12Q1/6806 ,  C12Q1/6881 ,  C40B30/06 ,  C12N5/00

Abstract: Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

公开(公告)号：US20250019864A1

公开(公告)日：2025-01-16

申请号：US18606205

申请日：2024-03-15

Applicant: Life Technologies Corporation

Inventor： Timothy Looney ,  Geoffrey Lowman ,  Lifeng Lin

IPC: C40B50/06 ,  C07K14/725 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6883

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20240401240A1

公开(公告)日：2024-12-05

申请号：US18742590

申请日：2024-06-13

Applicant: 10x Genomics, Inc.

Inventor： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC: C40B30/04 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20240401128A1

公开(公告)日：2024-12-05

申请号：US18627305

申请日：2024-04-04

Applicant: Fred Hutchinson Cancer Center

Inventor： Jason H. BIELAS

IPC: C12Q1/6874 ,  C12N15/10 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12Q1/6827 ,  C12Q1/6869 ,  C40B40/08 ,  C40B50/06

Abstract: The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.

公开(公告)号：US20240376639A1

公开(公告)日：2024-11-14

申请号：US18785856

申请日：2024-07-26

Applicant: ELASMOGEN LTD

Inventor： Caroline Jane BARELLE ,  Marina KOVALEVA ,  Laura Ann FERGUSON ,  Obinna Chukwuemeka UBAH ,  Andrew Justin Radcliffe PORTER

IPC: C40B50/06 ,  C07K14/195 ,  C12N15/10

Abstract: The present invention relates to a synthetic library of humanised antigen specific binding molecules derived from a member of a species in the Elasmobranchii subclass, processes for the production thereof 5 and specific antigen specific binding molecules isolated from said library. The present invention also relates to the multi-domain specific binding molecules comprising humanised Ig-like Novel Antigen Receptor variable domains (VNARs). Specific binding domains that bind to Tumour Necrosis Factor alpha (TNFα) are also provided.

公开(公告)号：US20240271328A1

公开(公告)日：2024-08-15

申请号：US18582106

申请日：2024-02-20

Applicant: SEQONCE BIOSCIENCES, INC.

Inventor： Joseph DUNHAM

IPC: C40B50/06 ,  C12N15/10 ,  C40B70/00 ,  C40B80/00

CPC classification number: C40B50/06 ,  C12N15/1068 ,  C40B70/00 ,  C40B80/00

Abstract: Rapid methods, capable of being performed in a single reaction tube, are described herein for constructing libraries for high-throughput polynucleotide sequencing applications, such as next generation sequencing (NGS) applications. Oligonucleotide probes include chemically-active groups at their 5′ or 3′ ends, or both, to facilitate the cleavage of their 5′ or 3′ ends, or both, following their hybridization to the single-stranded ends of frayed template fragments. Cleavage of probe ends reveal single-stranded regions at the ends of the hybridized fragments. Adaptors, specific to these ends, are ligated to the hybridized probe/template fragments, and blunt end fragments are ligated to blunt ends of hybridized probe/template fragments, if present, to generate the adaptor-ligated fragments of the library.

公开(公告)号：US12031176B2

公开(公告)日：2024-07-09

申请号：US16940517

申请日：2020-07-28

Applicant: HITACHI HIGH-TECH CORPORATION

Inventor： Masataka Shirai ,  Tomoyuki Sakai ,  Kenko Uchida

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6837 ,  C40B40/08 ,  C40B50/06 ,  G01N33/543

CPC classification number: C12Q1/6837 ,  C12N15/1003 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C40B40/08 ,  C40B50/06 ,  G01N33/54306

Abstract: The purpose of the present invention is to provide a single cell analysis device in which the improvement of the nucleic acid capturing efficiency and the improvement of the cell capturing efficiency are both achieved and a highly accurate single cell analysis data is thereby obtained. The present invention relates to an improvement of a cell analysis device including a two-dimensional array chip having a plurality of cell capture parts capable of capturing a single cell in each of the capture parts, and nucleic acid capture parts corresponding to the respective cell capture parts, the nucleic acid capture parts being capable of capturing a nucleic acid extracted from the cell captured by the cell capture part.

公开(公告)号：US12024797B2

公开(公告)日：2024-07-02

申请号：US17133418

申请日：2020-12-23

Applicant: GRAIL, LLC

Inventor： Matthew H. Larson ,  Hyunsung John Kim ,  Nick Eattock ,  Xiao Yang

IPC: C40B50/06 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874

CPC classification number: C40B50/06 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2535/122 ,  C12Q2565/514 ,  C12N15/1093 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: Aspects of the invention relate to methods for preparing and analyzing a sequencing library from a mixed cell-free DNA (cfDNA) sample, wherein the mixed sample includes double-stranded DNA (dsDNA), damaged dsDNA (e.g., nicked dsDNA), and single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared from dsDNA alone.

公开(公告)号：US12018254B2

公开(公告)日：2024-06-25

申请号：US17386176

申请日：2021-07-27

Applicant: Wisconsin Alumni Research Foundation

Inventor： Srivatsan Raman ,  Phil Huss

IPC: C40B50/06 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1093 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/20 ,  C12N2795/10052

Abstract: Described herein is a method of preparing an unbiased library of phage variants, comprising (a) preparing a population of “acceptor phage”; (b) removing an endogenous target gene and inserting gene variants into the acceptor phage genomes; (c) enriching the recombined phages; and (d) expressing the library for selection. The acceptor phage is a lytic phage comprising a synthetic genome wherein the target gene of interest is flanked by recombinase sites. The acceptor phage infects a first host bacteria expressing a recombination plasmid facilitating recombination. The phages then infect a second host bacteria expressing a counterselection system that accumulates recombined phage variants and selecting against non-recombined phages. The accumulated phage variants infect a third host bacteria. The phage library may then be sequenced and characterized.

公开(公告)号：US12001962B2

公开(公告)日：2024-06-04

申请号：US18230273

申请日：2023-08-04

Applicant: CATALOG TECHNOLOGIES, INC.

Inventor： Nathaniel Roquet ,  Hyunjun Park ,  Swapnil P. Bhatia ,  Darren R. Link

IPC: G06F16/00 ,  C12N9/22 ,  C12N15/10 ,  C40B50/06 ,  G06F16/22 ,  G06F16/245 ,  G06N3/123 ,  G11C13/00 ,  G16B30/00 ,  G16B30/20 ,  G16B50/00 ,  G16B99/00

CPC classification number: G06N3/123 ,  C12N9/22 ,  C12N15/1031 ,  C12N15/1089 ,  C40B50/06 ,  G06F16/2272 ,  G06F16/245 ,  G11C13/0019 ,  G16B30/00 ,  G16B30/20 ,  G16B50/00 ,  G16B99/00 ,  C12N2310/20

Abstract: Methods and systems for encoding digital information in nucleic acid (e.g., deoxyribonucleic acid) molecules without base-by-base synthesis, by encoding bit-value information in the presence or absence of unique nucleic acid sequences within a pool, comprising specifying each bit location in a bit-stream with a unique nucleic sequence and specifying the bit value at that location by the presence or absence of the corresponding unique nucleic acid sequence in the pool. But, more generally, specifying unique bytes in a bytestream by unique subsets of nucleic acid sequences. Also disclosed are methods for generating unique nucleic acid sequences without base-by-base synthesis using combinatorial genomic strategies (e.g., assembly of multiple nucleic acid sequences or enzymatic-based editing of nucleic acid sequences).