公开(公告)号：US20220010372A1

公开(公告)日：2022-01-13

申请号：US17484910

申请日：2021-09-24

Applicant: GRAIL, LLC

Inventor： Matthew Larson ,  H. John Kim ,  Nick Eattock ,  Arash Jamshidi

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.

公开(公告)号：US12024797B2

公开(公告)日：2024-07-02

申请号：US17133418

申请日：2020-12-23

Applicant: GRAIL, LLC

Inventor： Matthew H. Larson ,  Hyunsung John Kim ,  Nick Eattock ,  Xiao Yang

IPC: C40B50/06 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874

CPC classification number: C40B50/06 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2535/122 ,  C12Q2565/514 ,  C12N15/1093 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: Aspects of the invention relate to methods for preparing and analyzing a sequencing library from a mixed cell-free DNA (cfDNA) sample, wherein the mixed sample includes double-stranded DNA (dsDNA), damaged dsDNA (e.g., nicked dsDNA), and single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared from dsDNA alone.

公开(公告)号：US11180801B2

公开(公告)日：2021-11-23

申请号：US16177105

申请日：2018-10-31

Applicant: GRAIL, LLC

Inventor： Matthew Larson ,  H. John Kim ,  Nick Eattock ,  Arash Jamshidi

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.