公开(公告)号：US12100479B2

公开(公告)日：2024-09-24

申请号：US16490288

申请日：2018-03-01

Applicant: BlueDot LLC ,  TRIAD NATIONAL SECURITY, LLC

Inventor： Momchilo Vuyisich ,  Jason Gans ,  Niels Klitgord ,  Po-E Li ,  Patrick Chain

IPC: G16B20/20 ,  C12N15/10 ,  C12Q1/6874 ,  G16B10/00 ,  G16B30/10 ,  G16B30/20

CPC classification number: G16B20/20 ,  C12N15/1096 ,  C12Q1/6874 ,  G16B10/00 ,  G16B30/10 ,  G16B30/20

Abstract: Provided are methods and systems for metagenomic analysis. Methods involve generating libraries and databases of taxonomic signatures informative of taxonomic information in a heterogeneous microbial sample. Methods also include identification of microorganisms and biochemical activities in the sample, including identification at a plurality of taxonomic levels.

公开(公告)号：US20240287506A1

公开(公告)日：2024-08-29

申请号：US18572473

申请日：2022-05-31

Applicant: WESTLAKE UNIVERSITY

Inventor： Zhike LU ,  Lijia MA

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C12Q1/686

CPC classification number: C12N15/1068 ,  C12N9/22 ,  C12N15/1096 ,  C12N15/11 ,  C12N15/86 ,  C12Q1/686 ,  C12N2310/20 ,  C12N2740/15043

Abstract: Provided is a library construction method based on long overhang sequence ligation, a library constructed by the library construction method, for example, a CRISPR library of pair-specific multiplexed guide RNA (gRNA) combinations, and a use of the library. The CRISPR library of pair-specific multiplexed gRNA combinations can be used to simultaneously perturb multiple (e.g., 4) pre-designed targets in CRISPR/Cas9 screening. The CRISPR library of pair-specific multiplexed gRNA combinations can be a powerful tool for studying the combinatorial outcomes from coordinated gene behaviors.

公开(公告)号：US12071711B2

公开(公告)日：2024-08-27

申请号：US17470434

申请日：2021-09-09

Applicant: Illumina Cambridge Limited

Inventor： Niall Anthony Gormley ,  Geoffrey Paul Smith ,  David Bentley ,  Roberto Rigatti ,  Shujun Luo

IPC: C40B50/06 ,  C12N15/10 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B40/06

CPC classification number: C40B50/06 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B40/06 ,  Y10T436/143333 ,  C12Q1/6855 ,  C12Q2565/525 ,  C12Q2525/313 ,  C12N15/1093 ,  C12Q2525/313 ,  C12Q2565/525

Abstract: The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.

公开(公告)号：US20240271228A1

公开(公告)日：2024-08-15

申请号：US18400972

申请日：2023-12-29

Applicant: HYPERCELL TECHNOLOGIES

Inventor： Bruno Georges JACTEL ,  Alexander Michael PROKUP ,  Terri WASMOEN

IPC: C12Q1/70 ,  C12N15/10 ,  C12Q1/24 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/64

CPC classification number: C12Q1/70 ,  C12N15/1096 ,  C12Q1/24 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/6486 ,  C12Q2600/158

Abstract: Disclosed are compositions, devices and methods for processing biological and environmental samples for rapid detection of a gene of interest in a biological sample and uses thereof.

公开(公告)号：US20240263179A1

公开(公告)日：2024-08-08

申请号：US18566561

申请日：2022-06-03

Applicant: TAKEDA PHARMACEUTICALS U.S.A., INC.

Inventor： Nessan Anthony BERMINGHAM ,  Brian R. BETTENCOURT

IPC: C12N15/113 ,  C12N15/10

CPC classification number: C12N15/113 ,  C12N15/1096 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/346

Abstract: The present disclosure features useful compositions and methods to treat nucleotide repeat expansion disorders, e.g., in a subject in need thereof. In some aspects, the compositions and methods described herein are useful in the treatment of disorders associated with MSH3 activity.

公开(公告)号：US20240257914A1

公开(公告)日：2024-08-01

申请号：US18561321

申请日：2022-05-17

Applicant: MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT

Inventor： JONATHAN ALLES ,  NIKOLAOS KARAISKOS ,  NIKOLAUS RAJEWSKY ,  GIUSEPPE MACINO ,  ANDREW WOEHLER ,  ENES SENEL ,  STEFANO ABBIATI ,  SALAH AYOUB ,  SEBASTIAN EHRIG ,  STEPHAN PREIBISCH

IPC: G16B30/10 ,  C12N15/10 ,  C12Q1/6834 ,  G16B40/20 ,  G16B45/00

CPC classification number: G16B30/10 ,  C12N15/1096 ,  C12Q1/6834 ,  G16B40/20 ,  G16B45/00

Abstract: The present invention relates to a computer-implemented analysis of spatial abundance of poly-A containing RNA in a tissue sample, comprising the steps of: (i) obtaining imaging data and the sequencing data, (ii) registering the imaging data and detecting the beads, and employing a first machine learning method to obtain a first barcode set from the imaging data, (iii) processing the sequencing data to obtain a second barcode set from the sequencing data, (iv) processing the first and second barcode sets by an optimal transport framework and/or supervised machine learning to match the data sets to each other and to obtain matched barcodes, (v) outputting based on the matched barcodes a matrix holding the expression values for each gene that was identified in each bead found in the data.

公开(公告)号：US20240254460A1

公开(公告)日：2024-08-01

申请号：US17254376

申请日：2020-11-13

Applicant: Daan Gene Co., Ltd.

Inventor： Xiwen JIANG ,  Aishan LIU ,  Zhouquan WANG ,  Sangsang ZHENG ,  Jiguang ZHAO ,  Xianlan LIAN ,  Xiaocheng XIE ,  Xuelan LU ,  Wei ZHANG ,  Haojian XU ,  Zhikeng FANG ,  Zhijian WU

IPC: C12N9/12 ,  C12N15/10

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12Y207/07049

Abstract: The present invention provides a high temperature resistant reverse transcriptase mutant and its use. In particular, the present invention constructs a reverse transcriptase (M-MLV) mutant library, and the mutants with improved thermostability and higher amplification efficiency are finally screened out by stepwise screening. Under high temperature conditions, the reverse transcription efficiency of the reverse transcriptase mutant of the present invention is greatly improved compared to the wild type.

公开(公告)号：US12049623B2

公开(公告)日：2024-07-30

申请号：US17892686

申请日：2022-08-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Paul A. Feldstein ,  James E. Lincoln

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12N15/1093 ,  C12N15/1051 ,  C12N15/1096 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q2521/107

Abstract: Provided are constructs and methods useful for the screening and identification of polynucleotide sequences of interest, in particular promoters, RNA stability modifying sequences and transcriptional modifying sequences.

公开(公告)号：US20240229015A1

公开(公告)日：2024-07-11

申请号：US18611981

申请日：2024-03-21

Applicant: FUJIFILM Corporation

Inventor： Yukiko ISHII ,  Jo TOMINAGA ,  Shiori KIHARA ,  Takahiro HOHSAKA ,  Takayoshi WATANABE

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1062 ,  C12N15/1044 ,  C12N15/1096 ,  C12N15/113

Abstract: Provided are a tRNA obtained by modifying a tRNA for tryptophan of Mycoplasma pneumoniae, the tRNA including a UUA as an anticodon to pair with a UAA codon, and an application of the tRNA.

公开(公告)号：US20240200134A1

公开(公告)日：2024-06-20

申请号：US18448121

申请日：2023-08-10

Applicant: Massachusetts Institute of Technology

Inventor： J. Christopher Love ,  Todd Michael Gierahn ,  Alexander K. Shalek ,  Marc Havens Wadsworth ,  Travis K. Hughes

IPC: C12Q1/6874 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6806 ,  G03F7/20

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851

Abstract: This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and case of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.